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Rapid MALDI mass spectrometry imaging for surgical pathology

Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing and data acquisition can take over 30 min, limiting its...

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Autores principales: Basu, Sankha S., Regan, Michael S., Randall, Elizabeth C., Abdelmoula, Walid M., Clark, Amanda R., Gimenez-Cassina Lopez, Begoña, Cornett, Dale S., Haase, Andreas, Santagata, Sandro, Agar, Nathalie Y. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609678/
https://www.ncbi.nlm.nih.gov/pubmed/31286061
http://dx.doi.org/10.1038/s41698-019-0089-y
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author Basu, Sankha S.
Regan, Michael S.
Randall, Elizabeth C.
Abdelmoula, Walid M.
Clark, Amanda R.
Gimenez-Cassina Lopez, Begoña
Cornett, Dale S.
Haase, Andreas
Santagata, Sandro
Agar, Nathalie Y. R.
author_facet Basu, Sankha S.
Regan, Michael S.
Randall, Elizabeth C.
Abdelmoula, Walid M.
Clark, Amanda R.
Gimenez-Cassina Lopez, Begoña
Cornett, Dale S.
Haase, Andreas
Santagata, Sandro
Agar, Nathalie Y. R.
author_sort Basu, Sankha S.
collection PubMed
description Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing and data acquisition can take over 30 min, limiting its clinical utility for intraoperative diagnostics. We present a rapid MALDI MSI method, completed under 5 min, including sample preparation and analysis, providing a workflow compatible with the clinical frozen section procedure.
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spelling pubmed-66096782019-07-08 Rapid MALDI mass spectrometry imaging for surgical pathology Basu, Sankha S. Regan, Michael S. Randall, Elizabeth C. Abdelmoula, Walid M. Clark, Amanda R. Gimenez-Cassina Lopez, Begoña Cornett, Dale S. Haase, Andreas Santagata, Sandro Agar, Nathalie Y. R. NPJ Precis Oncol Brief Communication Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing and data acquisition can take over 30 min, limiting its clinical utility for intraoperative diagnostics. We present a rapid MALDI MSI method, completed under 5 min, including sample preparation and analysis, providing a workflow compatible with the clinical frozen section procedure. Nature Publishing Group UK 2019-07-04 /pmc/articles/PMC6609678/ /pubmed/31286061 http://dx.doi.org/10.1038/s41698-019-0089-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Brief Communication
Basu, Sankha S.
Regan, Michael S.
Randall, Elizabeth C.
Abdelmoula, Walid M.
Clark, Amanda R.
Gimenez-Cassina Lopez, Begoña
Cornett, Dale S.
Haase, Andreas
Santagata, Sandro
Agar, Nathalie Y. R.
Rapid MALDI mass spectrometry imaging for surgical pathology
title Rapid MALDI mass spectrometry imaging for surgical pathology
title_full Rapid MALDI mass spectrometry imaging for surgical pathology
title_fullStr Rapid MALDI mass spectrometry imaging for surgical pathology
title_full_unstemmed Rapid MALDI mass spectrometry imaging for surgical pathology
title_short Rapid MALDI mass spectrometry imaging for surgical pathology
title_sort rapid maldi mass spectrometry imaging for surgical pathology
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609678/
https://www.ncbi.nlm.nih.gov/pubmed/31286061
http://dx.doi.org/10.1038/s41698-019-0089-y
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