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Leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL

Gastric cancer (GC) is a common cause of cancer‐related death worldwide. As a result of the lack of reliable diagnostic or prognostic biomarkers for GC, patient prognosis is still poor. Therefore, there is an urgent need for studies examining the underlying pathogenesis of GC in order to find effect...

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Detalles Bibliográficos
Autores principales: Liu, Bofang, Zhang, Ye, Fan, Yibo, Wang, Shuo, Li, Zhi, Deng, Mingming, Li, Ce, Wang, Jin, Ma, Rui, Wang, Xiaoxun, Wang, Yan, Xu, Ling, Hou, Kezuo, Che, Xiaofang, Liu, Yunpeng, Qu, Xiujuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609808/
https://www.ncbi.nlm.nih.gov/pubmed/31087525
http://dx.doi.org/10.1111/cas.14042
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author Liu, Bofang
Zhang, Ye
Fan, Yibo
Wang, Shuo
Li, Zhi
Deng, Mingming
Li, Ce
Wang, Jin
Ma, Rui
Wang, Xiaoxun
Wang, Yan
Xu, Ling
Hou, Kezuo
Che, Xiaofang
Liu, Yunpeng
Qu, Xiujuan
author_facet Liu, Bofang
Zhang, Ye
Fan, Yibo
Wang, Shuo
Li, Zhi
Deng, Mingming
Li, Ce
Wang, Jin
Ma, Rui
Wang, Xiaoxun
Wang, Yan
Xu, Ling
Hou, Kezuo
Che, Xiaofang
Liu, Yunpeng
Qu, Xiujuan
author_sort Liu, Bofang
collection PubMed
description Gastric cancer (GC) is a common cause of cancer‐related death worldwide. As a result of the lack of reliable diagnostic or prognostic biomarkers for GC, patient prognosis is still poor. Therefore, there is an urgent need for studies examining the underlying pathogenesis of GC in order to find effective biomarkers. LRRN1 (leucine‐rich repeat neuronal protein‐1) is a type I transmembrane protein that plays an important role in the process of nerve development and regeneration. However, its role in cancer, especially in GC, remains unclear. In the present study, we found that LRRN1 expression is upregulated in GC tissues and that high LRRN1 expression is associated with poor prognosis. siRNA and shRNA‐mediated knockdowns of LRRN1 expression promoted GC cell apoptosis and activation of the Fas/FasL pathway. LRRN1 knockdown also resulted in upregulation of JUN, a subunit of the transcription factor AP‐1 (activator protein‐1). This suggests that LRRN1 suppresses GC cell apoptosis by downregulating AP‐1, resulting in inhibition of the Fas/FasL pathway. These results confirm that LRRN1 plays a significant role in GC pathogenesis. Moreover, LRRN1 may be a potential prognostic biomarker and therapeutic target for GC.
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spelling pubmed-66098082019-07-15 Leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL Liu, Bofang Zhang, Ye Fan, Yibo Wang, Shuo Li, Zhi Deng, Mingming Li, Ce Wang, Jin Ma, Rui Wang, Xiaoxun Wang, Yan Xu, Ling Hou, Kezuo Che, Xiaofang Liu, Yunpeng Qu, Xiujuan Cancer Sci Original Articles Gastric cancer (GC) is a common cause of cancer‐related death worldwide. As a result of the lack of reliable diagnostic or prognostic biomarkers for GC, patient prognosis is still poor. Therefore, there is an urgent need for studies examining the underlying pathogenesis of GC in order to find effective biomarkers. LRRN1 (leucine‐rich repeat neuronal protein‐1) is a type I transmembrane protein that plays an important role in the process of nerve development and regeneration. However, its role in cancer, especially in GC, remains unclear. In the present study, we found that LRRN1 expression is upregulated in GC tissues and that high LRRN1 expression is associated with poor prognosis. siRNA and shRNA‐mediated knockdowns of LRRN1 expression promoted GC cell apoptosis and activation of the Fas/FasL pathway. LRRN1 knockdown also resulted in upregulation of JUN, a subunit of the transcription factor AP‐1 (activator protein‐1). This suggests that LRRN1 suppresses GC cell apoptosis by downregulating AP‐1, resulting in inhibition of the Fas/FasL pathway. These results confirm that LRRN1 plays a significant role in GC pathogenesis. Moreover, LRRN1 may be a potential prognostic biomarker and therapeutic target for GC. John Wiley and Sons Inc. 2019-06-10 2019-07 /pmc/articles/PMC6609808/ /pubmed/31087525 http://dx.doi.org/10.1111/cas.14042 Text en © 2019 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Liu, Bofang
Zhang, Ye
Fan, Yibo
Wang, Shuo
Li, Zhi
Deng, Mingming
Li, Ce
Wang, Jin
Ma, Rui
Wang, Xiaoxun
Wang, Yan
Xu, Ling
Hou, Kezuo
Che, Xiaofang
Liu, Yunpeng
Qu, Xiujuan
Leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL
title Leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL
title_full Leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL
title_fullStr Leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL
title_full_unstemmed Leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL
title_short Leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL
title_sort leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of fas/fasl
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609808/
https://www.ncbi.nlm.nih.gov/pubmed/31087525
http://dx.doi.org/10.1111/cas.14042
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