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MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer
Tumor metastasis is the leading cause of mortality in patients with advanced ovarian cancer. Myelin protein zero like 1 (MPZL1) is a transmembrane glycoprotein that promotes migration of hepatocellular carcinoma cells and is involved in extracellular matrix-induced signal transduction. However, the...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610034/ https://www.ncbi.nlm.nih.gov/pubmed/31233194 http://dx.doi.org/10.3892/or.2019.7199 |
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author | Chen, Danni Cao, Lei Wang, Xiaojie |
author_facet | Chen, Danni Cao, Lei Wang, Xiaojie |
author_sort | Chen, Danni |
collection | PubMed |
description | Tumor metastasis is the leading cause of mortality in patients with advanced ovarian cancer. Myelin protein zero like 1 (MPZL1) is a transmembrane glycoprotein that promotes migration of hepatocellular carcinoma cells and is involved in extracellular matrix-induced signal transduction. However, the functional role of MPZL1 in ovarian cancer has not been well elucidated. The present study conducted western blotting, phase-contrast imaging and immunohistochemistry to reveal the functions of MPZL1 in ovarian cancer. The present study demonstrated that the expression levels of MPZL1 were associated with malignant features of ovarian cancer. Furthermore, overexpression of MPZL1 significantly promoted cell proliferation, migration and invasion of ovarian cancer cells. Conversely, MPZL1 depletion by short hairpin RNA inhibited migration and invasion of ovarian cancer cells. In addition, this study demonstrated that phosphorylation of Src kinase was increased upon MPZL1 overexpression. Additionally, phosphorylation and activation of pro-metastatic proteins p130 and cortactin were induced by phosphorylated Src kinase. Collectively, these findings indicated that MPZL1 may be a novel pro-metastatic gene, which promotes tumor cell proliferation and migration through Src-mediated phosphorylation of p130 and cortactin in ovarian cancer. |
format | Online Article Text |
id | pubmed-6610034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-66100342019-07-23 MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer Chen, Danni Cao, Lei Wang, Xiaojie Oncol Rep Articles Tumor metastasis is the leading cause of mortality in patients with advanced ovarian cancer. Myelin protein zero like 1 (MPZL1) is a transmembrane glycoprotein that promotes migration of hepatocellular carcinoma cells and is involved in extracellular matrix-induced signal transduction. However, the functional role of MPZL1 in ovarian cancer has not been well elucidated. The present study conducted western blotting, phase-contrast imaging and immunohistochemistry to reveal the functions of MPZL1 in ovarian cancer. The present study demonstrated that the expression levels of MPZL1 were associated with malignant features of ovarian cancer. Furthermore, overexpression of MPZL1 significantly promoted cell proliferation, migration and invasion of ovarian cancer cells. Conversely, MPZL1 depletion by short hairpin RNA inhibited migration and invasion of ovarian cancer cells. In addition, this study demonstrated that phosphorylation of Src kinase was increased upon MPZL1 overexpression. Additionally, phosphorylation and activation of pro-metastatic proteins p130 and cortactin were induced by phosphorylated Src kinase. Collectively, these findings indicated that MPZL1 may be a novel pro-metastatic gene, which promotes tumor cell proliferation and migration through Src-mediated phosphorylation of p130 and cortactin in ovarian cancer. D.A. Spandidos 2019-08 2019-06-12 /pmc/articles/PMC6610034/ /pubmed/31233194 http://dx.doi.org/10.3892/or.2019.7199 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Chen, Danni Cao, Lei Wang, Xiaojie MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer |
title | MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer |
title_full | MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer |
title_fullStr | MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer |
title_full_unstemmed | MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer |
title_short | MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer |
title_sort | mpzl1 promotes tumor cell proliferation and migration via activation of src kinase in ovarian cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610034/ https://www.ncbi.nlm.nih.gov/pubmed/31233194 http://dx.doi.org/10.3892/or.2019.7199 |
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