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MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer

Tumor metastasis is the leading cause of mortality in patients with advanced ovarian cancer. Myelin protein zero like 1 (MPZL1) is a transmembrane glycoprotein that promotes migration of hepatocellular carcinoma cells and is involved in extracellular matrix-induced signal transduction. However, the...

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Detalles Bibliográficos
Autores principales: Chen, Danni, Cao, Lei, Wang, Xiaojie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610034/
https://www.ncbi.nlm.nih.gov/pubmed/31233194
http://dx.doi.org/10.3892/or.2019.7199
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author Chen, Danni
Cao, Lei
Wang, Xiaojie
author_facet Chen, Danni
Cao, Lei
Wang, Xiaojie
author_sort Chen, Danni
collection PubMed
description Tumor metastasis is the leading cause of mortality in patients with advanced ovarian cancer. Myelin protein zero like 1 (MPZL1) is a transmembrane glycoprotein that promotes migration of hepatocellular carcinoma cells and is involved in extracellular matrix-induced signal transduction. However, the functional role of MPZL1 in ovarian cancer has not been well elucidated. The present study conducted western blotting, phase-contrast imaging and immunohistochemistry to reveal the functions of MPZL1 in ovarian cancer. The present study demonstrated that the expression levels of MPZL1 were associated with malignant features of ovarian cancer. Furthermore, overexpression of MPZL1 significantly promoted cell proliferation, migration and invasion of ovarian cancer cells. Conversely, MPZL1 depletion by short hairpin RNA inhibited migration and invasion of ovarian cancer cells. In addition, this study demonstrated that phosphorylation of Src kinase was increased upon MPZL1 overexpression. Additionally, phosphorylation and activation of pro-metastatic proteins p130 and cortactin were induced by phosphorylated Src kinase. Collectively, these findings indicated that MPZL1 may be a novel pro-metastatic gene, which promotes tumor cell proliferation and migration through Src-mediated phosphorylation of p130 and cortactin in ovarian cancer.
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spelling pubmed-66100342019-07-23 MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer Chen, Danni Cao, Lei Wang, Xiaojie Oncol Rep Articles Tumor metastasis is the leading cause of mortality in patients with advanced ovarian cancer. Myelin protein zero like 1 (MPZL1) is a transmembrane glycoprotein that promotes migration of hepatocellular carcinoma cells and is involved in extracellular matrix-induced signal transduction. However, the functional role of MPZL1 in ovarian cancer has not been well elucidated. The present study conducted western blotting, phase-contrast imaging and immunohistochemistry to reveal the functions of MPZL1 in ovarian cancer. The present study demonstrated that the expression levels of MPZL1 were associated with malignant features of ovarian cancer. Furthermore, overexpression of MPZL1 significantly promoted cell proliferation, migration and invasion of ovarian cancer cells. Conversely, MPZL1 depletion by short hairpin RNA inhibited migration and invasion of ovarian cancer cells. In addition, this study demonstrated that phosphorylation of Src kinase was increased upon MPZL1 overexpression. Additionally, phosphorylation and activation of pro-metastatic proteins p130 and cortactin were induced by phosphorylated Src kinase. Collectively, these findings indicated that MPZL1 may be a novel pro-metastatic gene, which promotes tumor cell proliferation and migration through Src-mediated phosphorylation of p130 and cortactin in ovarian cancer. D.A. Spandidos 2019-08 2019-06-12 /pmc/articles/PMC6610034/ /pubmed/31233194 http://dx.doi.org/10.3892/or.2019.7199 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Chen, Danni
Cao, Lei
Wang, Xiaojie
MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer
title MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer
title_full MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer
title_fullStr MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer
title_full_unstemmed MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer
title_short MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer
title_sort mpzl1 promotes tumor cell proliferation and migration via activation of src kinase in ovarian cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610034/
https://www.ncbi.nlm.nih.gov/pubmed/31233194
http://dx.doi.org/10.3892/or.2019.7199
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