Cargando…
CHD1L is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma
Chromodomain helicase/ATPase DNA-binding protein 1-like gene (CHD1L) is a new oncogene which has been confirmed to be crucial to the progression of many solid tumors. In the present study, the expression of CHD1L was found to be upregulated in intrahepatic cholangiocarcinoma (ICC), which was signifi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610041/ https://www.ncbi.nlm.nih.gov/pubmed/31173252 http://dx.doi.org/10.3892/or.2019.7174 |
_version_ | 1783432429277544448 |
---|---|
author | Li, Shimiao Chai, Yi Ding, Yanbao Yuan, Tinghao Wu, Changwen Huang, Changwen |
author_facet | Li, Shimiao Chai, Yi Ding, Yanbao Yuan, Tinghao Wu, Changwen Huang, Changwen |
author_sort | Li, Shimiao |
collection | PubMed |
description | Chromodomain helicase/ATPase DNA-binding protein 1-like gene (CHD1L) is a new oncogene which has been confirmed to be crucial to the progression of many solid tumors. In the present study, the expression of CHD1L was found to be upregulated in intrahepatic cholangiocarcinoma (ICC), which was significantly associated with histological differentiation (P=0.011), vascular invasion (P=0.002), lymph node metastasis (P=0.008) and TNM stage (P=0.001). Kaplan-Meier survival analysis revealed that ICC patients with positive CHD1L expression had shorter overall and disease-free survival than those with negative CHD1L expression. Functional study found that CHD1L exhibited strong oncogenic roles, including increased cell growth by CCK-8 assay, colony formation by plate colony formation assay, G1/S transition by flow cytometry and tumor formation in nude mice. In addition, RNAi-mediated silencing of CHD1L inhibited ICC invasion and metastasis by wound healing, Transwell migration and Matrigel invasion assays in vitro and in vivo. Collectively, our results show that CHD1L is upregulated and promotes the proliferation and metastasis of ICC cells. CHD1L acts as an oncogene and may be a prognostic factor or therapeutic target for patients with ICC. |
format | Online Article Text |
id | pubmed-6610041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-66100412019-07-23 CHD1L is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma Li, Shimiao Chai, Yi Ding, Yanbao Yuan, Tinghao Wu, Changwen Huang, Changwen Oncol Rep Articles Chromodomain helicase/ATPase DNA-binding protein 1-like gene (CHD1L) is a new oncogene which has been confirmed to be crucial to the progression of many solid tumors. In the present study, the expression of CHD1L was found to be upregulated in intrahepatic cholangiocarcinoma (ICC), which was significantly associated with histological differentiation (P=0.011), vascular invasion (P=0.002), lymph node metastasis (P=0.008) and TNM stage (P=0.001). Kaplan-Meier survival analysis revealed that ICC patients with positive CHD1L expression had shorter overall and disease-free survival than those with negative CHD1L expression. Functional study found that CHD1L exhibited strong oncogenic roles, including increased cell growth by CCK-8 assay, colony formation by plate colony formation assay, G1/S transition by flow cytometry and tumor formation in nude mice. In addition, RNAi-mediated silencing of CHD1L inhibited ICC invasion and metastasis by wound healing, Transwell migration and Matrigel invasion assays in vitro and in vivo. Collectively, our results show that CHD1L is upregulated and promotes the proliferation and metastasis of ICC cells. CHD1L acts as an oncogene and may be a prognostic factor or therapeutic target for patients with ICC. D.A. Spandidos 2019-08 2019-05-28 /pmc/articles/PMC6610041/ /pubmed/31173252 http://dx.doi.org/10.3892/or.2019.7174 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Shimiao Chai, Yi Ding, Yanbao Yuan, Tinghao Wu, Changwen Huang, Changwen CHD1L is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma |
title | CHD1L is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma |
title_full | CHD1L is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma |
title_fullStr | CHD1L is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma |
title_full_unstemmed | CHD1L is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma |
title_short | CHD1L is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma |
title_sort | chd1l is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610041/ https://www.ncbi.nlm.nih.gov/pubmed/31173252 http://dx.doi.org/10.3892/or.2019.7174 |
work_keys_str_mv | AT lishimiao chd1lisassociatedwithpoorsurvivalandpromotestheproliferationandmetastasisofintrahepaticcholangiocarcinoma AT chaiyi chd1lisassociatedwithpoorsurvivalandpromotestheproliferationandmetastasisofintrahepaticcholangiocarcinoma AT dingyanbao chd1lisassociatedwithpoorsurvivalandpromotestheproliferationandmetastasisofintrahepaticcholangiocarcinoma AT yuantinghao chd1lisassociatedwithpoorsurvivalandpromotestheproliferationandmetastasisofintrahepaticcholangiocarcinoma AT wuchangwen chd1lisassociatedwithpoorsurvivalandpromotestheproliferationandmetastasisofintrahepaticcholangiocarcinoma AT huangchangwen chd1lisassociatedwithpoorsurvivalandpromotestheproliferationandmetastasisofintrahepaticcholangiocarcinoma |