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Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

CRISPR-Cas systems inherently multiplex through CRISPR arrays—whether to defend against different invaders or mediate multi-target editing, regulation, imaging, or sensing. However, arrays remain difficult to generate due to their reoccurring repeat sequences. Here, we report a modular, one-pot sche...

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Detalles Bibliográficos
Autores principales:	Liao, Chunyu, Ttofali, Fani, Slotkowski, Rebecca A., Denny, Steven R., Cecil, Taylor D., Leenay, Ryan T., Keung, Albert J., Beisel, Chase L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610086/ https://www.ncbi.nlm.nih.gov/pubmed/31270316 http://dx.doi.org/10.1038/s41467-019-10747-3

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