Cargando…

Hypertonic solution-induced preconditioning reduces inflammation and mortality rate

BACKGROUND: Dysregulated inflammatory response is common cause of organ damage in critical care patients. Preconditioning/tolerance is a strategy to prevent exacerbated inflammation. The aim of this study is to analyze hypertonic saline 7.5% as a potential inducer of preconditioning that protect fro...

Descripción completa

Detalles Bibliográficos
Autores principales: Pimentel, Rosangela Nascimento, Petroni, Ricardo Costa, Barbeiro, Hermes Vieira, Barbeiro, Denise Frediani, Andrade, Mariana Macedo, Ariga, Suely Kumini, Soriano, Francisco Garcia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610871/
https://www.ncbi.nlm.nih.gov/pubmed/31312113
http://dx.doi.org/10.1186/s12950-019-0220-4
_version_ 1783432581893586944
author Pimentel, Rosangela Nascimento
Petroni, Ricardo Costa
Barbeiro, Hermes Vieira
Barbeiro, Denise Frediani
Andrade, Mariana Macedo
Ariga, Suely Kumini
Soriano, Francisco Garcia
author_facet Pimentel, Rosangela Nascimento
Petroni, Ricardo Costa
Barbeiro, Hermes Vieira
Barbeiro, Denise Frediani
Andrade, Mariana Macedo
Ariga, Suely Kumini
Soriano, Francisco Garcia
author_sort Pimentel, Rosangela Nascimento
collection PubMed
description BACKGROUND: Dysregulated inflammatory response is common cause of organ damage in critical care patients. Preconditioning/tolerance is a strategy to prevent exacerbated inflammation. The aim of this study is to analyze hypertonic saline 7.5% as a potential inducer of preconditioning that protect from a lethal dose of LPS and modulates systemic inflammatory profile in mice. METHODS: Male Balb/C mice received intravenous (i.v.) injections of Hypertonic solution (NaCl 7.5%) (0.8 ml) for 3 days, on day 8th was challenged with LPS 15 mg/kg. Controls with Saline 0.9%, urea and sorbitol were performed. Microarray of mRNA expression was analyzed from HS versus saline from macrophages to identified the pathways activated by HS. RESULTS: HS preconditioning reduced mortality after LPS injection as well reduced the cytokines release in plasma of the animals challenged by LPS. In order to check how HS induces a preconditioning state we measured plasma cytokines after each HS infusion. Repeated HS injections induced a state of preconditioning that reprograms the inflammatory response, resulting in reduced inflammatory cytokine production. A microarray of mRNA demonstrated that Hypertonic solution increased the expression of several genes in special Mapkbp1 and Atf3. CONCLUSION: hypertonic solution induces preconditioning/tolerance reducing mortality and inflammatory response after LPS challenge.
format Online
Article
Text
id pubmed-6610871
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-66108712019-07-16 Hypertonic solution-induced preconditioning reduces inflammation and mortality rate Pimentel, Rosangela Nascimento Petroni, Ricardo Costa Barbeiro, Hermes Vieira Barbeiro, Denise Frediani Andrade, Mariana Macedo Ariga, Suely Kumini Soriano, Francisco Garcia J Inflamm (Lond) Research BACKGROUND: Dysregulated inflammatory response is common cause of organ damage in critical care patients. Preconditioning/tolerance is a strategy to prevent exacerbated inflammation. The aim of this study is to analyze hypertonic saline 7.5% as a potential inducer of preconditioning that protect from a lethal dose of LPS and modulates systemic inflammatory profile in mice. METHODS: Male Balb/C mice received intravenous (i.v.) injections of Hypertonic solution (NaCl 7.5%) (0.8 ml) for 3 days, on day 8th was challenged with LPS 15 mg/kg. Controls with Saline 0.9%, urea and sorbitol were performed. Microarray of mRNA expression was analyzed from HS versus saline from macrophages to identified the pathways activated by HS. RESULTS: HS preconditioning reduced mortality after LPS injection as well reduced the cytokines release in plasma of the animals challenged by LPS. In order to check how HS induces a preconditioning state we measured plasma cytokines after each HS infusion. Repeated HS injections induced a state of preconditioning that reprograms the inflammatory response, resulting in reduced inflammatory cytokine production. A microarray of mRNA demonstrated that Hypertonic solution increased the expression of several genes in special Mapkbp1 and Atf3. CONCLUSION: hypertonic solution induces preconditioning/tolerance reducing mortality and inflammatory response after LPS challenge. BioMed Central 2019-07-03 /pmc/articles/PMC6610871/ /pubmed/31312113 http://dx.doi.org/10.1186/s12950-019-0220-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Pimentel, Rosangela Nascimento
Petroni, Ricardo Costa
Barbeiro, Hermes Vieira
Barbeiro, Denise Frediani
Andrade, Mariana Macedo
Ariga, Suely Kumini
Soriano, Francisco Garcia
Hypertonic solution-induced preconditioning reduces inflammation and mortality rate
title Hypertonic solution-induced preconditioning reduces inflammation and mortality rate
title_full Hypertonic solution-induced preconditioning reduces inflammation and mortality rate
title_fullStr Hypertonic solution-induced preconditioning reduces inflammation and mortality rate
title_full_unstemmed Hypertonic solution-induced preconditioning reduces inflammation and mortality rate
title_short Hypertonic solution-induced preconditioning reduces inflammation and mortality rate
title_sort hypertonic solution-induced preconditioning reduces inflammation and mortality rate
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610871/
https://www.ncbi.nlm.nih.gov/pubmed/31312113
http://dx.doi.org/10.1186/s12950-019-0220-4
work_keys_str_mv AT pimentelrosangelanascimento hypertonicsolutioninducedpreconditioningreducesinflammationandmortalityrate
AT petroniricardocosta hypertonicsolutioninducedpreconditioningreducesinflammationandmortalityrate
AT barbeirohermesvieira hypertonicsolutioninducedpreconditioningreducesinflammationandmortalityrate
AT barbeirodenisefrediani hypertonicsolutioninducedpreconditioningreducesinflammationandmortalityrate
AT andrademarianamacedo hypertonicsolutioninducedpreconditioningreducesinflammationandmortalityrate
AT arigasuelykumini hypertonicsolutioninducedpreconditioningreducesinflammationandmortalityrate
AT sorianofranciscogarcia hypertonicsolutioninducedpreconditioningreducesinflammationandmortalityrate