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Genome-wide identification and functional analysis of oleosin genes in Brassica napus L.
BACKGROUND: Rapeseed is the third largest oil seed crop in the world. The seeds of this plant store lipids in oil bodies, and oleosin is the most important structural protein in oil bodies. However, the function of oleosin in oil crops has received little attention. RESULTS: In the present study, 48...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610931/ https://www.ncbi.nlm.nih.gov/pubmed/31272381 http://dx.doi.org/10.1186/s12870-019-1891-y |
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author | Chen, Kang Yin, Yongtai Liu, Si Guo, Zhenyi Zhang, Kai Liang, Yu Zhang, Lina Zhao, Weiguo Chao, Hongbo Li, Maoteng |
author_facet | Chen, Kang Yin, Yongtai Liu, Si Guo, Zhenyi Zhang, Kai Liang, Yu Zhang, Lina Zhao, Weiguo Chao, Hongbo Li, Maoteng |
author_sort | Chen, Kang |
collection | PubMed |
description | BACKGROUND: Rapeseed is the third largest oil seed crop in the world. The seeds of this plant store lipids in oil bodies, and oleosin is the most important structural protein in oil bodies. However, the function of oleosin in oil crops has received little attention. RESULTS: In the present study, 48 oleosin sequences from the Brassica napus genome were identified and divided into four lineages (T, U, SH, SL). Synteny analysis revealed that most of the oleosin genes were conserved, and all of these genes experienced purifying selection during evolution. Three and four important oleosin genes from Arabidopsis and B. napus, respectively, were cloned and analyzed for function in Arabidopsis. Overexpression of these oleosin genes in Arabidopsis increased the seed oil content slightly, except for BnaOLE3. Further analysis revealed that the average oil body size of the transgenic seeds was slightly larger than that of the wild type (WT), except for BnaOLE1. The fatty acid profiles showed that the linoleic acid content (13.3% at most) increased and the peanut acid content (11% at most) decreased in the transgenic lines. In addition, the seed size and thousand-seed weight (TSW) also increased in the transgenic lines, which could lead to increased total lipid production. CONCLUSION: We identified oleosin genes in the B. napus genome, and overexpression of oleosin in Arabidopsis seeds increased the seed weight and linoleic acid content (13.3% at most). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1891-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6610931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-66109312019-07-16 Genome-wide identification and functional analysis of oleosin genes in Brassica napus L. Chen, Kang Yin, Yongtai Liu, Si Guo, Zhenyi Zhang, Kai Liang, Yu Zhang, Lina Zhao, Weiguo Chao, Hongbo Li, Maoteng BMC Plant Biol Research Article BACKGROUND: Rapeseed is the third largest oil seed crop in the world. The seeds of this plant store lipids in oil bodies, and oleosin is the most important structural protein in oil bodies. However, the function of oleosin in oil crops has received little attention. RESULTS: In the present study, 48 oleosin sequences from the Brassica napus genome were identified and divided into four lineages (T, U, SH, SL). Synteny analysis revealed that most of the oleosin genes were conserved, and all of these genes experienced purifying selection during evolution. Three and four important oleosin genes from Arabidopsis and B. napus, respectively, were cloned and analyzed for function in Arabidopsis. Overexpression of these oleosin genes in Arabidopsis increased the seed oil content slightly, except for BnaOLE3. Further analysis revealed that the average oil body size of the transgenic seeds was slightly larger than that of the wild type (WT), except for BnaOLE1. The fatty acid profiles showed that the linoleic acid content (13.3% at most) increased and the peanut acid content (11% at most) decreased in the transgenic lines. In addition, the seed size and thousand-seed weight (TSW) also increased in the transgenic lines, which could lead to increased total lipid production. CONCLUSION: We identified oleosin genes in the B. napus genome, and overexpression of oleosin in Arabidopsis seeds increased the seed weight and linoleic acid content (13.3% at most). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1891-y) contains supplementary material, which is available to authorized users. BioMed Central 2019-07-04 /pmc/articles/PMC6610931/ /pubmed/31272381 http://dx.doi.org/10.1186/s12870-019-1891-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Chen, Kang Yin, Yongtai Liu, Si Guo, Zhenyi Zhang, Kai Liang, Yu Zhang, Lina Zhao, Weiguo Chao, Hongbo Li, Maoteng Genome-wide identification and functional analysis of oleosin genes in Brassica napus L. |
title | Genome-wide identification and functional analysis of oleosin genes in Brassica napus L. |
title_full | Genome-wide identification and functional analysis of oleosin genes in Brassica napus L. |
title_fullStr | Genome-wide identification and functional analysis of oleosin genes in Brassica napus L. |
title_full_unstemmed | Genome-wide identification and functional analysis of oleosin genes in Brassica napus L. |
title_short | Genome-wide identification and functional analysis of oleosin genes in Brassica napus L. |
title_sort | genome-wide identification and functional analysis of oleosin genes in brassica napus l. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610931/ https://www.ncbi.nlm.nih.gov/pubmed/31272381 http://dx.doi.org/10.1186/s12870-019-1891-y |
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