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Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility

Single nucleotide polymorphisms (SNPs) in TLR genes may serve as a crucial marker for early susceptibility of various cancers including cervical cancer. The present study was therefore designed to ascertain the role of TLR4 and TLR9 SNPs and haplotypes to hrHPV infection and cervical cancer suscepti...

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Autores principales: Pandey, Nilesh O., Chauhan, Alex V., Raithatha, Nitin S., Patel, Purvi K., Khandelwal, Ronak, Desai, Ajesh N., Choxi, Yesha, Kapadia, Rutul S., Jain, Neeraj D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611874/
https://www.ncbi.nlm.nih.gov/pubmed/31278284
http://dx.doi.org/10.1038/s41598-019-46077-z
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author Pandey, Nilesh O.
Chauhan, Alex V.
Raithatha, Nitin S.
Patel, Purvi K.
Khandelwal, Ronak
Desai, Ajesh N.
Choxi, Yesha
Kapadia, Rutul S.
Jain, Neeraj D.
author_facet Pandey, Nilesh O.
Chauhan, Alex V.
Raithatha, Nitin S.
Patel, Purvi K.
Khandelwal, Ronak
Desai, Ajesh N.
Choxi, Yesha
Kapadia, Rutul S.
Jain, Neeraj D.
author_sort Pandey, Nilesh O.
collection PubMed
description Single nucleotide polymorphisms (SNPs) in TLR genes may serve as a crucial marker for early susceptibility of various cancers including cervical cancer. The present study was therefore designed to ascertain the role of TLR4 and TLR9 SNPs and haplotypes to hrHPV infection and cervical cancer susceptibility. The study included 110 cervical cancer biopsies and 141 cervical smears from age-matched healthy controls of Gujarati ethnicity of Western India. hrHPV 16 and 18 were detected using Real-time PCR. Eight SNPs, four each in TLR4 and TLR9 were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Allele-Specific PCR. HPV 16 and 18 were detected in 68% cervical cancer cases. TLR4 rs4986790, rs1927911 and TLR9 rs187084 showed association with HPV 16/18 infection. CC and CT genotypes of TLR4 rs11536889 and rs1927911 respectively, and TC, CC genotypes of TLR9 rs187084, as well as minor alleles of TLR4 rs4986790 and TLR9 rs187084, were associated with the increased risk of cervical cancer. Stage-wise analysis revealed TLR9 rs187084 and rs352140 to be associated with early-stage cancer. TLR4 haplotype GTAC and TLR9 haplotype GATC were associated with the increased risk of cervical cancer while TLR4 haplotype GCAG was associated with the decreased risk. TLR4 haplotype GCAG and TLR9 haplotype GATC showed association with increased susceptibility to hrHPV infection. In conclusion, the present study revealed association of TLR4 and TLR9 polymorphisms and haplotypes with hrHPV infection and cervical cancer risk. Further evaluation of a larger sample size covering diverse ethnic populations globally is warranted.
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spelling pubmed-66118742019-07-15 Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility Pandey, Nilesh O. Chauhan, Alex V. Raithatha, Nitin S. Patel, Purvi K. Khandelwal, Ronak Desai, Ajesh N. Choxi, Yesha Kapadia, Rutul S. Jain, Neeraj D. Sci Rep Article Single nucleotide polymorphisms (SNPs) in TLR genes may serve as a crucial marker for early susceptibility of various cancers including cervical cancer. The present study was therefore designed to ascertain the role of TLR4 and TLR9 SNPs and haplotypes to hrHPV infection and cervical cancer susceptibility. The study included 110 cervical cancer biopsies and 141 cervical smears from age-matched healthy controls of Gujarati ethnicity of Western India. hrHPV 16 and 18 were detected using Real-time PCR. Eight SNPs, four each in TLR4 and TLR9 were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Allele-Specific PCR. HPV 16 and 18 were detected in 68% cervical cancer cases. TLR4 rs4986790, rs1927911 and TLR9 rs187084 showed association with HPV 16/18 infection. CC and CT genotypes of TLR4 rs11536889 and rs1927911 respectively, and TC, CC genotypes of TLR9 rs187084, as well as minor alleles of TLR4 rs4986790 and TLR9 rs187084, were associated with the increased risk of cervical cancer. Stage-wise analysis revealed TLR9 rs187084 and rs352140 to be associated with early-stage cancer. TLR4 haplotype GTAC and TLR9 haplotype GATC were associated with the increased risk of cervical cancer while TLR4 haplotype GCAG was associated with the decreased risk. TLR4 haplotype GCAG and TLR9 haplotype GATC showed association with increased susceptibility to hrHPV infection. In conclusion, the present study revealed association of TLR4 and TLR9 polymorphisms and haplotypes with hrHPV infection and cervical cancer risk. Further evaluation of a larger sample size covering diverse ethnic populations globally is warranted. Nature Publishing Group UK 2019-07-05 /pmc/articles/PMC6611874/ /pubmed/31278284 http://dx.doi.org/10.1038/s41598-019-46077-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pandey, Nilesh O.
Chauhan, Alex V.
Raithatha, Nitin S.
Patel, Purvi K.
Khandelwal, Ronak
Desai, Ajesh N.
Choxi, Yesha
Kapadia, Rutul S.
Jain, Neeraj D.
Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility
title Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility
title_full Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility
title_fullStr Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility
title_full_unstemmed Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility
title_short Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility
title_sort association of tlr4 and tlr9 polymorphisms and haplotypes with cervical cancer susceptibility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611874/
https://www.ncbi.nlm.nih.gov/pubmed/31278284
http://dx.doi.org/10.1038/s41598-019-46077-z
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