Cargando…
Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility
Single nucleotide polymorphisms (SNPs) in TLR genes may serve as a crucial marker for early susceptibility of various cancers including cervical cancer. The present study was therefore designed to ascertain the role of TLR4 and TLR9 SNPs and haplotypes to hrHPV infection and cervical cancer suscepti...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611874/ https://www.ncbi.nlm.nih.gov/pubmed/31278284 http://dx.doi.org/10.1038/s41598-019-46077-z |
_version_ | 1783432780934283264 |
---|---|
author | Pandey, Nilesh O. Chauhan, Alex V. Raithatha, Nitin S. Patel, Purvi K. Khandelwal, Ronak Desai, Ajesh N. Choxi, Yesha Kapadia, Rutul S. Jain, Neeraj D. |
author_facet | Pandey, Nilesh O. Chauhan, Alex V. Raithatha, Nitin S. Patel, Purvi K. Khandelwal, Ronak Desai, Ajesh N. Choxi, Yesha Kapadia, Rutul S. Jain, Neeraj D. |
author_sort | Pandey, Nilesh O. |
collection | PubMed |
description | Single nucleotide polymorphisms (SNPs) in TLR genes may serve as a crucial marker for early susceptibility of various cancers including cervical cancer. The present study was therefore designed to ascertain the role of TLR4 and TLR9 SNPs and haplotypes to hrHPV infection and cervical cancer susceptibility. The study included 110 cervical cancer biopsies and 141 cervical smears from age-matched healthy controls of Gujarati ethnicity of Western India. hrHPV 16 and 18 were detected using Real-time PCR. Eight SNPs, four each in TLR4 and TLR9 were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Allele-Specific PCR. HPV 16 and 18 were detected in 68% cervical cancer cases. TLR4 rs4986790, rs1927911 and TLR9 rs187084 showed association with HPV 16/18 infection. CC and CT genotypes of TLR4 rs11536889 and rs1927911 respectively, and TC, CC genotypes of TLR9 rs187084, as well as minor alleles of TLR4 rs4986790 and TLR9 rs187084, were associated with the increased risk of cervical cancer. Stage-wise analysis revealed TLR9 rs187084 and rs352140 to be associated with early-stage cancer. TLR4 haplotype GTAC and TLR9 haplotype GATC were associated with the increased risk of cervical cancer while TLR4 haplotype GCAG was associated with the decreased risk. TLR4 haplotype GCAG and TLR9 haplotype GATC showed association with increased susceptibility to hrHPV infection. In conclusion, the present study revealed association of TLR4 and TLR9 polymorphisms and haplotypes with hrHPV infection and cervical cancer risk. Further evaluation of a larger sample size covering diverse ethnic populations globally is warranted. |
format | Online Article Text |
id | pubmed-6611874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66118742019-07-15 Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility Pandey, Nilesh O. Chauhan, Alex V. Raithatha, Nitin S. Patel, Purvi K. Khandelwal, Ronak Desai, Ajesh N. Choxi, Yesha Kapadia, Rutul S. Jain, Neeraj D. Sci Rep Article Single nucleotide polymorphisms (SNPs) in TLR genes may serve as a crucial marker for early susceptibility of various cancers including cervical cancer. The present study was therefore designed to ascertain the role of TLR4 and TLR9 SNPs and haplotypes to hrHPV infection and cervical cancer susceptibility. The study included 110 cervical cancer biopsies and 141 cervical smears from age-matched healthy controls of Gujarati ethnicity of Western India. hrHPV 16 and 18 were detected using Real-time PCR. Eight SNPs, four each in TLR4 and TLR9 were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Allele-Specific PCR. HPV 16 and 18 were detected in 68% cervical cancer cases. TLR4 rs4986790, rs1927911 and TLR9 rs187084 showed association with HPV 16/18 infection. CC and CT genotypes of TLR4 rs11536889 and rs1927911 respectively, and TC, CC genotypes of TLR9 rs187084, as well as minor alleles of TLR4 rs4986790 and TLR9 rs187084, were associated with the increased risk of cervical cancer. Stage-wise analysis revealed TLR9 rs187084 and rs352140 to be associated with early-stage cancer. TLR4 haplotype GTAC and TLR9 haplotype GATC were associated with the increased risk of cervical cancer while TLR4 haplotype GCAG was associated with the decreased risk. TLR4 haplotype GCAG and TLR9 haplotype GATC showed association with increased susceptibility to hrHPV infection. In conclusion, the present study revealed association of TLR4 and TLR9 polymorphisms and haplotypes with hrHPV infection and cervical cancer risk. Further evaluation of a larger sample size covering diverse ethnic populations globally is warranted. Nature Publishing Group UK 2019-07-05 /pmc/articles/PMC6611874/ /pubmed/31278284 http://dx.doi.org/10.1038/s41598-019-46077-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pandey, Nilesh O. Chauhan, Alex V. Raithatha, Nitin S. Patel, Purvi K. Khandelwal, Ronak Desai, Ajesh N. Choxi, Yesha Kapadia, Rutul S. Jain, Neeraj D. Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility |
title | Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility |
title_full | Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility |
title_fullStr | Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility |
title_full_unstemmed | Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility |
title_short | Association of TLR4 and TLR9 polymorphisms and haplotypes with cervical cancer susceptibility |
title_sort | association of tlr4 and tlr9 polymorphisms and haplotypes with cervical cancer susceptibility |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611874/ https://www.ncbi.nlm.nih.gov/pubmed/31278284 http://dx.doi.org/10.1038/s41598-019-46077-z |
work_keys_str_mv | AT pandeynilesho associationoftlr4andtlr9polymorphismsandhaplotypeswithcervicalcancersusceptibility AT chauhanalexv associationoftlr4andtlr9polymorphismsandhaplotypeswithcervicalcancersusceptibility AT raithathanitins associationoftlr4andtlr9polymorphismsandhaplotypeswithcervicalcancersusceptibility AT patelpurvik associationoftlr4andtlr9polymorphismsandhaplotypeswithcervicalcancersusceptibility AT khandelwalronak associationoftlr4andtlr9polymorphismsandhaplotypeswithcervicalcancersusceptibility AT desaiajeshn associationoftlr4andtlr9polymorphismsandhaplotypeswithcervicalcancersusceptibility AT choxiyesha associationoftlr4andtlr9polymorphismsandhaplotypeswithcervicalcancersusceptibility AT kapadiarutuls associationoftlr4andtlr9polymorphismsandhaplotypeswithcervicalcancersusceptibility AT jainneerajd associationoftlr4andtlr9polymorphismsandhaplotypeswithcervicalcancersusceptibility |