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Synergistic mechanisms of Sanghuang–Danshen phytochemicals on postprandial vascular dysfunction in healthy subjects: A network biology approach based on a clinical trial

With the increased risk of cardiovascular disease, the use of botanicals for vascular endothelial dysfunction has intensified. Here, we explored the synergistic mechanisms of Sanghuang–Danshen (SD) phytochemicals on the homeostatic protection against high-fat-induced vascular dysfunction in healthy...

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Autores principales: Lim, Yeni, Hwang, Woochang, Kim, Ji Yeon, Lee, Choong Hwan, Kim, Yong-Jae, Lee, Doheon, Kwon, Oran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611899/
https://www.ncbi.nlm.nih.gov/pubmed/31278329
http://dx.doi.org/10.1038/s41598-019-46289-3
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author Lim, Yeni
Hwang, Woochang
Kim, Ji Yeon
Lee, Choong Hwan
Kim, Yong-Jae
Lee, Doheon
Kwon, Oran
author_facet Lim, Yeni
Hwang, Woochang
Kim, Ji Yeon
Lee, Choong Hwan
Kim, Yong-Jae
Lee, Doheon
Kwon, Oran
author_sort Lim, Yeni
collection PubMed
description With the increased risk of cardiovascular disease, the use of botanicals for vascular endothelial dysfunction has intensified. Here, we explored the synergistic mechanisms of Sanghuang–Danshen (SD) phytochemicals on the homeostatic protection against high-fat-induced vascular dysfunction in healthy subjects, using a network biology approach, based on a randomised crossover clinical trial. Seventeen differential markers identified in blood samples taken at 0, 3 and 6 h post-treatment, together with 12SD phytochemicals, were mapped onto the network platform, termed the context-oriented directed associations. The resulting vascular sub-networks illustrated associations between 10 phytochemicals with 32 targets implicated in 143 metabolic/signalling pathways. The three key events included adhesion molecule production (ellagic acid, fumaric acid and cryptotanshinone; VCAM-1, ICAM-1 and PLA2G2A; fatty acid metabolism), platelet activation (ellagic acid, protocatechuic acid and tanshinone IIA; VEGFA, APAF1 and ATF3; mTOR, p53, Rap1 and VEGF signalling pathways) and endothelial inflammation (all phytochemicals, except cryptotanshinone; 29 targets, including TP53 and CASP3; MAPK and PI3K-Akt signalling pathways, among others). Our collective findings demonstrate a potential of SD to protect unintended risks of vascular dysfunction in healthy subjects, providing a deeper understanding of the complicated synergistic mechanisms of signature phytochemicals in SD.
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spelling pubmed-66118992019-07-15 Synergistic mechanisms of Sanghuang–Danshen phytochemicals on postprandial vascular dysfunction in healthy subjects: A network biology approach based on a clinical trial Lim, Yeni Hwang, Woochang Kim, Ji Yeon Lee, Choong Hwan Kim, Yong-Jae Lee, Doheon Kwon, Oran Sci Rep Article With the increased risk of cardiovascular disease, the use of botanicals for vascular endothelial dysfunction has intensified. Here, we explored the synergistic mechanisms of Sanghuang–Danshen (SD) phytochemicals on the homeostatic protection against high-fat-induced vascular dysfunction in healthy subjects, using a network biology approach, based on a randomised crossover clinical trial. Seventeen differential markers identified in blood samples taken at 0, 3 and 6 h post-treatment, together with 12SD phytochemicals, were mapped onto the network platform, termed the context-oriented directed associations. The resulting vascular sub-networks illustrated associations between 10 phytochemicals with 32 targets implicated in 143 metabolic/signalling pathways. The three key events included adhesion molecule production (ellagic acid, fumaric acid and cryptotanshinone; VCAM-1, ICAM-1 and PLA2G2A; fatty acid metabolism), platelet activation (ellagic acid, protocatechuic acid and tanshinone IIA; VEGFA, APAF1 and ATF3; mTOR, p53, Rap1 and VEGF signalling pathways) and endothelial inflammation (all phytochemicals, except cryptotanshinone; 29 targets, including TP53 and CASP3; MAPK and PI3K-Akt signalling pathways, among others). Our collective findings demonstrate a potential of SD to protect unintended risks of vascular dysfunction in healthy subjects, providing a deeper understanding of the complicated synergistic mechanisms of signature phytochemicals in SD. Nature Publishing Group UK 2019-07-05 /pmc/articles/PMC6611899/ /pubmed/31278329 http://dx.doi.org/10.1038/s41598-019-46289-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lim, Yeni
Hwang, Woochang
Kim, Ji Yeon
Lee, Choong Hwan
Kim, Yong-Jae
Lee, Doheon
Kwon, Oran
Synergistic mechanisms of Sanghuang–Danshen phytochemicals on postprandial vascular dysfunction in healthy subjects: A network biology approach based on a clinical trial
title Synergistic mechanisms of Sanghuang–Danshen phytochemicals on postprandial vascular dysfunction in healthy subjects: A network biology approach based on a clinical trial
title_full Synergistic mechanisms of Sanghuang–Danshen phytochemicals on postprandial vascular dysfunction in healthy subjects: A network biology approach based on a clinical trial
title_fullStr Synergistic mechanisms of Sanghuang–Danshen phytochemicals on postprandial vascular dysfunction in healthy subjects: A network biology approach based on a clinical trial
title_full_unstemmed Synergistic mechanisms of Sanghuang–Danshen phytochemicals on postprandial vascular dysfunction in healthy subjects: A network biology approach based on a clinical trial
title_short Synergistic mechanisms of Sanghuang–Danshen phytochemicals on postprandial vascular dysfunction in healthy subjects: A network biology approach based on a clinical trial
title_sort synergistic mechanisms of sanghuang–danshen phytochemicals on postprandial vascular dysfunction in healthy subjects: a network biology approach based on a clinical trial
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611899/
https://www.ncbi.nlm.nih.gov/pubmed/31278329
http://dx.doi.org/10.1038/s41598-019-46289-3
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