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Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site

BACKGROUND: Therapeutic vaccines against cervical cancer remain ineffective. Previously, we demonstrated that blocking the signalling of a cytokine, interleukin 10, at the time of immunisation elicited significantly higher numbers of antigen specific T cells and inhibited tumour growth in mice. RESU...

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Autores principales: Pan, Xuan, Ma, Bowei, You, Xinchao, Chen, Shu, Wu, Jialing, Wang, Tianfang, Walton, Shelley F., Yuan, Jianwei, Wu, Xiaolian, Chen, Guoqiang, Wang, Yuejian, Ni, Guoying, Liu, Xiaosong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6612097/
https://www.ncbi.nlm.nih.gov/pubmed/31277636
http://dx.doi.org/10.1186/s12906-019-2571-z
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author Pan, Xuan
Ma, Bowei
You, Xinchao
Chen, Shu
Wu, Jialing
Wang, Tianfang
Walton, Shelley F.
Yuan, Jianwei
Wu, Xiaolian
Chen, Guoqiang
Wang, Yuejian
Ni, Guoying
Liu, Xiaosong
author_facet Pan, Xuan
Ma, Bowei
You, Xinchao
Chen, Shu
Wu, Jialing
Wang, Tianfang
Walton, Shelley F.
Yuan, Jianwei
Wu, Xiaolian
Chen, Guoqiang
Wang, Yuejian
Ni, Guoying
Liu, Xiaosong
author_sort Pan, Xuan
collection PubMed
description BACKGROUND: Therapeutic vaccines against cervical cancer remain ineffective. Previously, we demonstrated that blocking the signalling of a cytokine, interleukin 10, at the time of immunisation elicited significantly higher numbers of antigen specific T cells and inhibited tumour growth in mice. RESULTS: In the current paper, we demonstrate, in a HPV16 E6/E7 transformed TC-1 tumour mouse model, that despite increased antigen specific T cell numbers, blocking IL-10 signalling at the time of immunisation does not increase the survival time of the TC-1 tumour bearing mice compared to mice receiving the same immunisation with no IL-10 signalling blockade. Moreover, the function of tumour infiltrating T cells isolated 3 weeks post TC-1 transplantation is more suppressed than those isolated 2 weeks after tumour inoculation. We demonstrate that synthesized caerin peptides, derived from amphibian skin secretions, 1) were able to inhibit TC-1 tumour growth both in vitro and in vivo; 2) are environmentally stable; and 3) promote the secretion of pro-inflammatory interlukine-6 by TC-1 cells. Notably caerin peptides were able to increase the survival time of TC-1 tumour bearing mice after therapeutic vaccination with a HPV16E7 peptide-based vaccine containing IL-10 inhibitor, via recruiting increased levels of T cells to the tumour site. CONCLUSION: Caerin peptides increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site.
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spelling pubmed-66120972019-07-16 Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site Pan, Xuan Ma, Bowei You, Xinchao Chen, Shu Wu, Jialing Wang, Tianfang Walton, Shelley F. Yuan, Jianwei Wu, Xiaolian Chen, Guoqiang Wang, Yuejian Ni, Guoying Liu, Xiaosong BMC Complement Altern Med Research Article BACKGROUND: Therapeutic vaccines against cervical cancer remain ineffective. Previously, we demonstrated that blocking the signalling of a cytokine, interleukin 10, at the time of immunisation elicited significantly higher numbers of antigen specific T cells and inhibited tumour growth in mice. RESULTS: In the current paper, we demonstrate, in a HPV16 E6/E7 transformed TC-1 tumour mouse model, that despite increased antigen specific T cell numbers, blocking IL-10 signalling at the time of immunisation does not increase the survival time of the TC-1 tumour bearing mice compared to mice receiving the same immunisation with no IL-10 signalling blockade. Moreover, the function of tumour infiltrating T cells isolated 3 weeks post TC-1 transplantation is more suppressed than those isolated 2 weeks after tumour inoculation. We demonstrate that synthesized caerin peptides, derived from amphibian skin secretions, 1) were able to inhibit TC-1 tumour growth both in vitro and in vivo; 2) are environmentally stable; and 3) promote the secretion of pro-inflammatory interlukine-6 by TC-1 cells. Notably caerin peptides were able to increase the survival time of TC-1 tumour bearing mice after therapeutic vaccination with a HPV16E7 peptide-based vaccine containing IL-10 inhibitor, via recruiting increased levels of T cells to the tumour site. CONCLUSION: Caerin peptides increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site. BioMed Central 2019-07-06 /pmc/articles/PMC6612097/ /pubmed/31277636 http://dx.doi.org/10.1186/s12906-019-2571-z Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Pan, Xuan
Ma, Bowei
You, Xinchao
Chen, Shu
Wu, Jialing
Wang, Tianfang
Walton, Shelley F.
Yuan, Jianwei
Wu, Xiaolian
Chen, Guoqiang
Wang, Yuejian
Ni, Guoying
Liu, Xiaosong
Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site
title Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site
title_full Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site
title_fullStr Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site
title_full_unstemmed Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site
title_short Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site
title_sort synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more t cells to the tumour site
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6612097/
https://www.ncbi.nlm.nih.gov/pubmed/31277636
http://dx.doi.org/10.1186/s12906-019-2571-z
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