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Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition

Burkholderia cenocepacia is an opportunistic bacterial pathogen that poses a significant threat to individuals with cystic fibrosis by provoking a strong inflammatory response within the lung. It possesses a type VI secretion system (T6SS), a secretory apparatus that can perforate the cellular membr...

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Autores principales: Spiewak, Helena L., Shastri, Sravanthi, Zhang, Lili, Schwager, Stephan, Eberl, Leo, Vergunst, Annette C., Thomas, Mark S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6612558/
https://www.ncbi.nlm.nih.gov/pubmed/30628184
http://dx.doi.org/10.1002/mbo3.774
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author Spiewak, Helena L.
Shastri, Sravanthi
Zhang, Lili
Schwager, Stephan
Eberl, Leo
Vergunst, Annette C.
Thomas, Mark S.
author_facet Spiewak, Helena L.
Shastri, Sravanthi
Zhang, Lili
Schwager, Stephan
Eberl, Leo
Vergunst, Annette C.
Thomas, Mark S.
author_sort Spiewak, Helena L.
collection PubMed
description Burkholderia cenocepacia is an opportunistic bacterial pathogen that poses a significant threat to individuals with cystic fibrosis by provoking a strong inflammatory response within the lung. It possesses a type VI secretion system (T6SS), a secretory apparatus that can perforate the cellular membrane of other bacterial species and/or eukaryotic targets, to deliver an arsenal of effector proteins. The B. cenocepacia T6SS (T6SS‐1) has been shown to be implicated in virulence in rats and contributes toward actin rearrangements and inflammasome activation in B. cenocepacia‐infected macrophages. Here, we present bioinformatics evidence to suggest that T6SS‐1 is the archetype T6SS in the Burkholderia genus. We show that B. cenocepacia T6SS‐1 is active under normal laboratory growth conditions and displays antibacterial activity against other Gram‐negative bacterial species. Moreover, B. cenocepacia T6SS‐1 is not required for virulence in three eukaryotic infection models. Bioinformatics analysis identified several candidate T6SS‐dependent effectors that may play a role in the antibacterial activity of B. cenocepacia T6SS‐1. We conclude that B. cenocepacia T6SS‐1 plays an important role in bacterial competition for this organism, and probably in all Burkholderia species that possess this system, thereby broadening the range of species that utilize the T6SS for this purpose.
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spelling pubmed-66125582019-07-16 Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition Spiewak, Helena L. Shastri, Sravanthi Zhang, Lili Schwager, Stephan Eberl, Leo Vergunst, Annette C. Thomas, Mark S. Microbiologyopen Original Articles Burkholderia cenocepacia is an opportunistic bacterial pathogen that poses a significant threat to individuals with cystic fibrosis by provoking a strong inflammatory response within the lung. It possesses a type VI secretion system (T6SS), a secretory apparatus that can perforate the cellular membrane of other bacterial species and/or eukaryotic targets, to deliver an arsenal of effector proteins. The B. cenocepacia T6SS (T6SS‐1) has been shown to be implicated in virulence in rats and contributes toward actin rearrangements and inflammasome activation in B. cenocepacia‐infected macrophages. Here, we present bioinformatics evidence to suggest that T6SS‐1 is the archetype T6SS in the Burkholderia genus. We show that B. cenocepacia T6SS‐1 is active under normal laboratory growth conditions and displays antibacterial activity against other Gram‐negative bacterial species. Moreover, B. cenocepacia T6SS‐1 is not required for virulence in three eukaryotic infection models. Bioinformatics analysis identified several candidate T6SS‐dependent effectors that may play a role in the antibacterial activity of B. cenocepacia T6SS‐1. We conclude that B. cenocepacia T6SS‐1 plays an important role in bacterial competition for this organism, and probably in all Burkholderia species that possess this system, thereby broadening the range of species that utilize the T6SS for this purpose. John Wiley and Sons Inc. 2019-01-09 /pmc/articles/PMC6612558/ /pubmed/30628184 http://dx.doi.org/10.1002/mbo3.774 Text en © 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Spiewak, Helena L.
Shastri, Sravanthi
Zhang, Lili
Schwager, Stephan
Eberl, Leo
Vergunst, Annette C.
Thomas, Mark S.
Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition
title Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition
title_full Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition
title_fullStr Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition
title_full_unstemmed Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition
title_short Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition
title_sort burkholderia cenocepacia utilizes a type vi secretion system for bacterial competition
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6612558/
https://www.ncbi.nlm.nih.gov/pubmed/30628184
http://dx.doi.org/10.1002/mbo3.774
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