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Follicular factors determining the developmental competence of porcine oocyte

PURPOSE: This study aimed to examine the relationship between granulosa cells (GCs), number of follicles, and the ability of follicular fluid to support in vitro growth of oocytes. METHODS: The culture medium was supplemented with follicular fluid (FF) collected from GC‐rich ovaries and GC‐poor ovar...

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Autores principales: Shibahara, Hidenori, Ishiguro, Ai, Shirasuna, Koumei, Kuwayama, Takehito, Iwata, Hisataka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613015/
https://www.ncbi.nlm.nih.gov/pubmed/31312104
http://dx.doi.org/10.1002/rmb2.12269
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author Shibahara, Hidenori
Ishiguro, Ai
Shirasuna, Koumei
Kuwayama, Takehito
Iwata, Hisataka
author_facet Shibahara, Hidenori
Ishiguro, Ai
Shirasuna, Koumei
Kuwayama, Takehito
Iwata, Hisataka
author_sort Shibahara, Hidenori
collection PubMed
description PURPOSE: This study aimed to examine the relationship between granulosa cells (GCs), number of follicles, and the ability of follicular fluid to support in vitro growth of oocytes. METHODS: The culture medium was supplemented with follicular fluid (FF) collected from GC‐rich ovaries and GC‐poor ovaries, and its effect on in vitro growth and quality of oocytes derived from early antral follicles (EAFs) was assessed. RESULTS: GC‐rich FF treatment enhanced oocyte growth and augmented changes in the chromatin configuration and lipid content of oocytes when compared to oocytes treated with GC‐poor FF. Moreover, oocytes treated with GC‐rich FF had a higher ability to progress to the blastocyst stage, than oocytes derived from large antral follicles (3‐5 mm in diameter). In addition, supplementation of the culture medium with either GC‐rich or GC‐poor FF enhanced histone acetylation in oocytes grown in vitro. CONCLUSION: GC‐rich FF contains key factors that support in vitro oocyte growth; hence, oocytes grown in GC‐rich FF medium had high developmental competence, which was comparative to the oocytes grown in vivo.
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spelling pubmed-66130152019-07-16 Follicular factors determining the developmental competence of porcine oocyte Shibahara, Hidenori Ishiguro, Ai Shirasuna, Koumei Kuwayama, Takehito Iwata, Hisataka Reprod Med Biol Original Articles PURPOSE: This study aimed to examine the relationship between granulosa cells (GCs), number of follicles, and the ability of follicular fluid to support in vitro growth of oocytes. METHODS: The culture medium was supplemented with follicular fluid (FF) collected from GC‐rich ovaries and GC‐poor ovaries, and its effect on in vitro growth and quality of oocytes derived from early antral follicles (EAFs) was assessed. RESULTS: GC‐rich FF treatment enhanced oocyte growth and augmented changes in the chromatin configuration and lipid content of oocytes when compared to oocytes treated with GC‐poor FF. Moreover, oocytes treated with GC‐rich FF had a higher ability to progress to the blastocyst stage, than oocytes derived from large antral follicles (3‐5 mm in diameter). In addition, supplementation of the culture medium with either GC‐rich or GC‐poor FF enhanced histone acetylation in oocytes grown in vitro. CONCLUSION: GC‐rich FF contains key factors that support in vitro oocyte growth; hence, oocytes grown in GC‐rich FF medium had high developmental competence, which was comparative to the oocytes grown in vivo. John Wiley and Sons Inc. 2019-04-10 /pmc/articles/PMC6613015/ /pubmed/31312104 http://dx.doi.org/10.1002/rmb2.12269 Text en © 2019 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Shibahara, Hidenori
Ishiguro, Ai
Shirasuna, Koumei
Kuwayama, Takehito
Iwata, Hisataka
Follicular factors determining the developmental competence of porcine oocyte
title Follicular factors determining the developmental competence of porcine oocyte
title_full Follicular factors determining the developmental competence of porcine oocyte
title_fullStr Follicular factors determining the developmental competence of porcine oocyte
title_full_unstemmed Follicular factors determining the developmental competence of porcine oocyte
title_short Follicular factors determining the developmental competence of porcine oocyte
title_sort follicular factors determining the developmental competence of porcine oocyte
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613015/
https://www.ncbi.nlm.nih.gov/pubmed/31312104
http://dx.doi.org/10.1002/rmb2.12269
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