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Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation
[Image: see text] DNA nanotechnology provides an avenue for the construction of rationally designed artificial assemblages with well-defined and tunable architectures. Shaped to mimic natural membrane-deforming proteins and equipped with membrane anchoring molecules, curved DNA nanostructures can re...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613048/ https://www.ncbi.nlm.nih.gov/pubmed/31252462 http://dx.doi.org/10.1021/acsami.9b05401 |
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author | Grome, Michael W Zhang, Zhao Lin, Chenxiang |
author_facet | Grome, Michael W Zhang, Zhao Lin, Chenxiang |
author_sort | Grome, Michael W |
collection | PubMed |
description | [Image: see text] DNA nanotechnology provides an avenue for the construction of rationally designed artificial assemblages with well-defined and tunable architectures. Shaped to mimic natural membrane-deforming proteins and equipped with membrane anchoring molecules, curved DNA nanostructures can reproduce subcellular membrane remodeling events such as vesicle tubulation in vitro. To systematically analyze how structural stiffness and membrane affinity of DNA nanostructures affect the membrane remodeling outcome, here we build DNA-origami curls with varying thickness and amphipathic peptide density, and have them polymerize into nanosprings on the surface of liposomes. We find that modestly reducing rigidity and maximizing the number of membrane anchors not only promote membrane binding and remodeling but also lead to the formation of lipid tubules with better defined diameters, highlighting the ability of programmable DNA-based constructs to controllably deform the membrane. |
format | Online Article Text |
id | pubmed-6613048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-66130482019-07-09 Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation Grome, Michael W Zhang, Zhao Lin, Chenxiang ACS Appl Mater Interfaces [Image: see text] DNA nanotechnology provides an avenue for the construction of rationally designed artificial assemblages with well-defined and tunable architectures. Shaped to mimic natural membrane-deforming proteins and equipped with membrane anchoring molecules, curved DNA nanostructures can reproduce subcellular membrane remodeling events such as vesicle tubulation in vitro. To systematically analyze how structural stiffness and membrane affinity of DNA nanostructures affect the membrane remodeling outcome, here we build DNA-origami curls with varying thickness and amphipathic peptide density, and have them polymerize into nanosprings on the surface of liposomes. We find that modestly reducing rigidity and maximizing the number of membrane anchors not only promote membrane binding and remodeling but also lead to the formation of lipid tubules with better defined diameters, highlighting the ability of programmable DNA-based constructs to controllably deform the membrane. American Chemical Society 2019-06-21 2019-07-03 /pmc/articles/PMC6613048/ /pubmed/31252462 http://dx.doi.org/10.1021/acsami.9b05401 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Grome, Michael W Zhang, Zhao Lin, Chenxiang Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation |
title | Stiffness
and Membrane Anchor Density Modulate DNA-Nanospring-Induced
Vesicle Tubulation |
title_full | Stiffness
and Membrane Anchor Density Modulate DNA-Nanospring-Induced
Vesicle Tubulation |
title_fullStr | Stiffness
and Membrane Anchor Density Modulate DNA-Nanospring-Induced
Vesicle Tubulation |
title_full_unstemmed | Stiffness
and Membrane Anchor Density Modulate DNA-Nanospring-Induced
Vesicle Tubulation |
title_short | Stiffness
and Membrane Anchor Density Modulate DNA-Nanospring-Induced
Vesicle Tubulation |
title_sort | stiffness
and membrane anchor density modulate dna-nanospring-induced
vesicle tubulation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613048/ https://www.ncbi.nlm.nih.gov/pubmed/31252462 http://dx.doi.org/10.1021/acsami.9b05401 |
work_keys_str_mv | AT gromemichaelw stiffnessandmembraneanchordensitymodulatednananospringinducedvesicletubulation AT zhangzhao stiffnessandmembraneanchordensitymodulatednananospringinducedvesicletubulation AT linchenxiang stiffnessandmembraneanchordensitymodulatednananospringinducedvesicletubulation |