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High-numerical-aperture macroscope optics for time-resolved experiments

A novel high-quality custom-made macroscope optics, dedicated to high-resolution time-resolved X-ray tomographic microscopy at the TOMCAT beamline at the Swiss Light Source (Paul Scherrer Institut, Switzerland), is introduced. The macroscope offers 4× magnification, has a very high numerical apertur...

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Autores principales: Bührer, Minna, Stampanoni, Marco, Rochet, Xavier, Büchi, Felix, Eller, Jens, Marone, Federica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613125/
https://www.ncbi.nlm.nih.gov/pubmed/31274440
http://dx.doi.org/10.1107/S1600577519004119
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author Bührer, Minna
Stampanoni, Marco
Rochet, Xavier
Büchi, Felix
Eller, Jens
Marone, Federica
author_facet Bührer, Minna
Stampanoni, Marco
Rochet, Xavier
Büchi, Felix
Eller, Jens
Marone, Federica
author_sort Bührer, Minna
collection PubMed
description A novel high-quality custom-made macroscope optics, dedicated to high-resolution time-resolved X-ray tomographic microscopy at the TOMCAT beamline at the Swiss Light Source (Paul Scherrer Institut, Switzerland), is introduced. The macroscope offers 4× magnification, has a very high numerical aperture of 0.35 and it is modular and highly flexible. It can be mounted both in a horizontal and vertical configuration, enabling imaging of tall samples close to the scintillator, to avoid edge-enhancement artefacts. The macroscope performance was characterized and compared with two existing in-house imaging setups, one dedicated to high spatial and one to high temporal resolution. The novel macroscope shows superior performance for both imaging settings compared with the previous systems. For the time-resolved setup, the macroscope is 4 times more efficient than the previous system and, at the same time, the spatial resolution is also increased by a factor of 6. For the high-spatial-resolution setup, the macroscope is up to 8.5 times more efficient with a moderate spatial resolution improvement (factor of 1.5). This high efficiency, increased spatial resolution and very high image quality offered by the novel macroscope optics will make 10–20 Hz high-resolution tomographic studies routinely possible, unlocking unprecedented possibilities for the tomographic investigations of dynamic processes and radiation-sensitive samples.
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spelling pubmed-66131252019-07-17 High-numerical-aperture macroscope optics for time-resolved experiments Bührer, Minna Stampanoni, Marco Rochet, Xavier Büchi, Felix Eller, Jens Marone, Federica J Synchrotron Radiat Research Papers A novel high-quality custom-made macroscope optics, dedicated to high-resolution time-resolved X-ray tomographic microscopy at the TOMCAT beamline at the Swiss Light Source (Paul Scherrer Institut, Switzerland), is introduced. The macroscope offers 4× magnification, has a very high numerical aperture of 0.35 and it is modular and highly flexible. It can be mounted both in a horizontal and vertical configuration, enabling imaging of tall samples close to the scintillator, to avoid edge-enhancement artefacts. The macroscope performance was characterized and compared with two existing in-house imaging setups, one dedicated to high spatial and one to high temporal resolution. The novel macroscope shows superior performance for both imaging settings compared with the previous systems. For the time-resolved setup, the macroscope is 4 times more efficient than the previous system and, at the same time, the spatial resolution is also increased by a factor of 6. For the high-spatial-resolution setup, the macroscope is up to 8.5 times more efficient with a moderate spatial resolution improvement (factor of 1.5). This high efficiency, increased spatial resolution and very high image quality offered by the novel macroscope optics will make 10–20 Hz high-resolution tomographic studies routinely possible, unlocking unprecedented possibilities for the tomographic investigations of dynamic processes and radiation-sensitive samples. International Union of Crystallography 2019-05-21 /pmc/articles/PMC6613125/ /pubmed/31274440 http://dx.doi.org/10.1107/S1600577519004119 Text en © Minna Bührer et al. 2019 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
Bührer, Minna
Stampanoni, Marco
Rochet, Xavier
Büchi, Felix
Eller, Jens
Marone, Federica
High-numerical-aperture macroscope optics for time-resolved experiments
title High-numerical-aperture macroscope optics for time-resolved experiments
title_full High-numerical-aperture macroscope optics for time-resolved experiments
title_fullStr High-numerical-aperture macroscope optics for time-resolved experiments
title_full_unstemmed High-numerical-aperture macroscope optics for time-resolved experiments
title_short High-numerical-aperture macroscope optics for time-resolved experiments
title_sort high-numerical-aperture macroscope optics for time-resolved experiments
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613125/
https://www.ncbi.nlm.nih.gov/pubmed/31274440
http://dx.doi.org/10.1107/S1600577519004119
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