Cargando…
High-numerical-aperture macroscope optics for time-resolved experiments
A novel high-quality custom-made macroscope optics, dedicated to high-resolution time-resolved X-ray tomographic microscopy at the TOMCAT beamline at the Swiss Light Source (Paul Scherrer Institut, Switzerland), is introduced. The macroscope offers 4× magnification, has a very high numerical apertur...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613125/ https://www.ncbi.nlm.nih.gov/pubmed/31274440 http://dx.doi.org/10.1107/S1600577519004119 |
_version_ | 1783432999299186688 |
---|---|
author | Bührer, Minna Stampanoni, Marco Rochet, Xavier Büchi, Felix Eller, Jens Marone, Federica |
author_facet | Bührer, Minna Stampanoni, Marco Rochet, Xavier Büchi, Felix Eller, Jens Marone, Federica |
author_sort | Bührer, Minna |
collection | PubMed |
description | A novel high-quality custom-made macroscope optics, dedicated to high-resolution time-resolved X-ray tomographic microscopy at the TOMCAT beamline at the Swiss Light Source (Paul Scherrer Institut, Switzerland), is introduced. The macroscope offers 4× magnification, has a very high numerical aperture of 0.35 and it is modular and highly flexible. It can be mounted both in a horizontal and vertical configuration, enabling imaging of tall samples close to the scintillator, to avoid edge-enhancement artefacts. The macroscope performance was characterized and compared with two existing in-house imaging setups, one dedicated to high spatial and one to high temporal resolution. The novel macroscope shows superior performance for both imaging settings compared with the previous systems. For the time-resolved setup, the macroscope is 4 times more efficient than the previous system and, at the same time, the spatial resolution is also increased by a factor of 6. For the high-spatial-resolution setup, the macroscope is up to 8.5 times more efficient with a moderate spatial resolution improvement (factor of 1.5). This high efficiency, increased spatial resolution and very high image quality offered by the novel macroscope optics will make 10–20 Hz high-resolution tomographic studies routinely possible, unlocking unprecedented possibilities for the tomographic investigations of dynamic processes and radiation-sensitive samples. |
format | Online Article Text |
id | pubmed-6613125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-66131252019-07-17 High-numerical-aperture macroscope optics for time-resolved experiments Bührer, Minna Stampanoni, Marco Rochet, Xavier Büchi, Felix Eller, Jens Marone, Federica J Synchrotron Radiat Research Papers A novel high-quality custom-made macroscope optics, dedicated to high-resolution time-resolved X-ray tomographic microscopy at the TOMCAT beamline at the Swiss Light Source (Paul Scherrer Institut, Switzerland), is introduced. The macroscope offers 4× magnification, has a very high numerical aperture of 0.35 and it is modular and highly flexible. It can be mounted both in a horizontal and vertical configuration, enabling imaging of tall samples close to the scintillator, to avoid edge-enhancement artefacts. The macroscope performance was characterized and compared with two existing in-house imaging setups, one dedicated to high spatial and one to high temporal resolution. The novel macroscope shows superior performance for both imaging settings compared with the previous systems. For the time-resolved setup, the macroscope is 4 times more efficient than the previous system and, at the same time, the spatial resolution is also increased by a factor of 6. For the high-spatial-resolution setup, the macroscope is up to 8.5 times more efficient with a moderate spatial resolution improvement (factor of 1.5). This high efficiency, increased spatial resolution and very high image quality offered by the novel macroscope optics will make 10–20 Hz high-resolution tomographic studies routinely possible, unlocking unprecedented possibilities for the tomographic investigations of dynamic processes and radiation-sensitive samples. International Union of Crystallography 2019-05-21 /pmc/articles/PMC6613125/ /pubmed/31274440 http://dx.doi.org/10.1107/S1600577519004119 Text en © Minna Bührer et al. 2019 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Research Papers Bührer, Minna Stampanoni, Marco Rochet, Xavier Büchi, Felix Eller, Jens Marone, Federica High-numerical-aperture macroscope optics for time-resolved experiments |
title | High-numerical-aperture macroscope optics for time-resolved experiments |
title_full | High-numerical-aperture macroscope optics for time-resolved experiments |
title_fullStr | High-numerical-aperture macroscope optics for time-resolved experiments |
title_full_unstemmed | High-numerical-aperture macroscope optics for time-resolved experiments |
title_short | High-numerical-aperture macroscope optics for time-resolved experiments |
title_sort | high-numerical-aperture macroscope optics for time-resolved experiments |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613125/ https://www.ncbi.nlm.nih.gov/pubmed/31274440 http://dx.doi.org/10.1107/S1600577519004119 |
work_keys_str_mv | AT buhrerminna highnumericalaperturemacroscopeopticsfortimeresolvedexperiments AT stampanonimarco highnumericalaperturemacroscopeopticsfortimeresolvedexperiments AT rochetxavier highnumericalaperturemacroscopeopticsfortimeresolvedexperiments AT buchifelix highnumericalaperturemacroscopeopticsfortimeresolvedexperiments AT ellerjens highnumericalaperturemacroscopeopticsfortimeresolvedexperiments AT maronefederica highnumericalaperturemacroscopeopticsfortimeresolvedexperiments |