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Development of multiplex PCR for neglected infectious diseases
Scrub typhus, murine typhus, and leptospirosis are widely neglected infectious diseases caused by Orientia tsutsugamushi, Rickettsia typhi, and pathogenic Leptospira spp., respectively. Patients usually present with non-specific symptoms and therefore are commonly diagnosed with acute undifferentiat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613674/ https://www.ncbi.nlm.nih.gov/pubmed/31283768 http://dx.doi.org/10.1371/journal.pntd.0007440 |
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author | Sea-liang, Nutchanart Sereemaspun, Amornpun Patarakul, Kanitha Gaywee, Jariyanart Rodkvamtook, Wuttikon Srisawat, Nattachai Wacharaplusadee, Supaporn Hemachudha, Thiravat |
author_facet | Sea-liang, Nutchanart Sereemaspun, Amornpun Patarakul, Kanitha Gaywee, Jariyanart Rodkvamtook, Wuttikon Srisawat, Nattachai Wacharaplusadee, Supaporn Hemachudha, Thiravat |
author_sort | Sea-liang, Nutchanart |
collection | PubMed |
description | Scrub typhus, murine typhus, and leptospirosis are widely neglected infectious diseases caused by Orientia tsutsugamushi, Rickettsia typhi, and pathogenic Leptospira spp., respectively. Patients usually present with non-specific symptoms and therefore are commonly diagnosed with acute undifferentiated febrile illness. Consequently, patients face delayed treatment and increased mortality. Antibody-based serological test currently used as gold standard has limitations due to insufficient antibody titers, especially in the early phase of infection. In this study, we aimed to develop multiplex PCR to combine 3 primer pairs that target specific genes encoding 56-kDa TSA of O. tsutsugamushi, 17-kDa antigen of R. typhi, and LipL32 of L. Interrogans and evaluate its performance in comparison to the standard serological tests. Using EDTA blood samples of known patients, the sensitivity and specificity of our multiplex PCR was 100% and 70%, respectively. In addition, the assay was able to diagnose the co-infection of scrub typhus and leptospirosis. The assay may be useful in identifying causative agents during the early phase of these diseases, enabling prompt and appropriate treatment. |
format | Online Article Text |
id | pubmed-6613674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-66136742019-07-23 Development of multiplex PCR for neglected infectious diseases Sea-liang, Nutchanart Sereemaspun, Amornpun Patarakul, Kanitha Gaywee, Jariyanart Rodkvamtook, Wuttikon Srisawat, Nattachai Wacharaplusadee, Supaporn Hemachudha, Thiravat PLoS Negl Trop Dis Research Article Scrub typhus, murine typhus, and leptospirosis are widely neglected infectious diseases caused by Orientia tsutsugamushi, Rickettsia typhi, and pathogenic Leptospira spp., respectively. Patients usually present with non-specific symptoms and therefore are commonly diagnosed with acute undifferentiated febrile illness. Consequently, patients face delayed treatment and increased mortality. Antibody-based serological test currently used as gold standard has limitations due to insufficient antibody titers, especially in the early phase of infection. In this study, we aimed to develop multiplex PCR to combine 3 primer pairs that target specific genes encoding 56-kDa TSA of O. tsutsugamushi, 17-kDa antigen of R. typhi, and LipL32 of L. Interrogans and evaluate its performance in comparison to the standard serological tests. Using EDTA blood samples of known patients, the sensitivity and specificity of our multiplex PCR was 100% and 70%, respectively. In addition, the assay was able to diagnose the co-infection of scrub typhus and leptospirosis. The assay may be useful in identifying causative agents during the early phase of these diseases, enabling prompt and appropriate treatment. Public Library of Science 2019-07-08 /pmc/articles/PMC6613674/ /pubmed/31283768 http://dx.doi.org/10.1371/journal.pntd.0007440 Text en © 2019 Sea-liang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Sea-liang, Nutchanart Sereemaspun, Amornpun Patarakul, Kanitha Gaywee, Jariyanart Rodkvamtook, Wuttikon Srisawat, Nattachai Wacharaplusadee, Supaporn Hemachudha, Thiravat Development of multiplex PCR for neglected infectious diseases |
title | Development of multiplex PCR for neglected infectious diseases |
title_full | Development of multiplex PCR for neglected infectious diseases |
title_fullStr | Development of multiplex PCR for neglected infectious diseases |
title_full_unstemmed | Development of multiplex PCR for neglected infectious diseases |
title_short | Development of multiplex PCR for neglected infectious diseases |
title_sort | development of multiplex pcr for neglected infectious diseases |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613674/ https://www.ncbi.nlm.nih.gov/pubmed/31283768 http://dx.doi.org/10.1371/journal.pntd.0007440 |
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