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A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography
BACKGROUND: A new simple, selective and accurate high-performance liquid chromatographic (HPLC) method utilising solid-phase extraction for the determination of pantoprazole in human plasma samples has been developed. AIM: The purpose of this paper was developing a new HPLC method suitable for the d...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Republic of Macedonia
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614261/ https://www.ncbi.nlm.nih.gov/pubmed/31316654 http://dx.doi.org/10.3889/oamjms.2019.237 |
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author | Zendelovska, Dragica Atanasovska, Emilija Gjorgjievska, Kalina Pavlovska, Kristina Jakjovski, Krume Zafirov, Dimce Trojacanec, Jasmina |
author_facet | Zendelovska, Dragica Atanasovska, Emilija Gjorgjievska, Kalina Pavlovska, Kristina Jakjovski, Krume Zafirov, Dimce Trojacanec, Jasmina |
author_sort | Zendelovska, Dragica |
collection | PubMed |
description | BACKGROUND: A new simple, selective and accurate high-performance liquid chromatographic (HPLC) method utilising solid-phase extraction for the determination of pantoprazole in human plasma samples has been developed. AIM: The purpose of this paper was developing a new HPLC method suitable for the determination of pantoprazole in plasma samples, which enables simple and rapid isolation and concentration of the analysed drug. METHODS: The chromatographic separation was accomplished on a LiChroCart LiChrospher 60 RP select B column using a mobile phase composed of 0.2 % (V/V) water solution of triethylamine (pH 7) and acetonitrile (58:42, V/V) followed by UV detection was at 280 nm. The solid-phase extraction method using LiChrolut RP-18 (200 mg, 3 ml) was applied to the obtained good separation of investigated drug from endogenous plasma components. Best results were achieved when plasma samples were buffered with 0.1 mol/L KH2PO4 (pH 9) before extraction, eluted and reconstituted with acetonitrile and 0.001 mol/L NaOH after extraction, respectively. RESULTS: The standard calibration curves showed good linearity within the range of 25.0-4000.0 ng/mL with a correlation coefficient greater than 0.996. Retention times of pantoprazole and internal standard, lansoprazole was 4.1 and 6.0 min respectively. The limit of quantification was 25.0 ng/mL. For intra- and inter-day precision relative standard deviations ranged from 4.2 to 9.3%. The relative errors for stability investigations were ranged from 0.12 to -10.5%. CONCLUSION: This method has good precision and accuracy and was successfully applied to the pharmacokinetic and bioequivalence study of 40 mg pantoprazole in healthy volunteers. |
format | Online Article Text |
id | pubmed-6614261 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Republic of Macedonia |
record_format | MEDLINE/PubMed |
spelling | pubmed-66142612019-07-17 A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography Zendelovska, Dragica Atanasovska, Emilija Gjorgjievska, Kalina Pavlovska, Kristina Jakjovski, Krume Zafirov, Dimce Trojacanec, Jasmina Open Access Maced J Med Sci Basic Science BACKGROUND: A new simple, selective and accurate high-performance liquid chromatographic (HPLC) method utilising solid-phase extraction for the determination of pantoprazole in human plasma samples has been developed. AIM: The purpose of this paper was developing a new HPLC method suitable for the determination of pantoprazole in plasma samples, which enables simple and rapid isolation and concentration of the analysed drug. METHODS: The chromatographic separation was accomplished on a LiChroCart LiChrospher 60 RP select B column using a mobile phase composed of 0.2 % (V/V) water solution of triethylamine (pH 7) and acetonitrile (58:42, V/V) followed by UV detection was at 280 nm. The solid-phase extraction method using LiChrolut RP-18 (200 mg, 3 ml) was applied to the obtained good separation of investigated drug from endogenous plasma components. Best results were achieved when plasma samples were buffered with 0.1 mol/L KH2PO4 (pH 9) before extraction, eluted and reconstituted with acetonitrile and 0.001 mol/L NaOH after extraction, respectively. RESULTS: The standard calibration curves showed good linearity within the range of 25.0-4000.0 ng/mL with a correlation coefficient greater than 0.996. Retention times of pantoprazole and internal standard, lansoprazole was 4.1 and 6.0 min respectively. The limit of quantification was 25.0 ng/mL. For intra- and inter-day precision relative standard deviations ranged from 4.2 to 9.3%. The relative errors for stability investigations were ranged from 0.12 to -10.5%. CONCLUSION: This method has good precision and accuracy and was successfully applied to the pharmacokinetic and bioequivalence study of 40 mg pantoprazole in healthy volunteers. Republic of Macedonia 2019-06-15 /pmc/articles/PMC6614261/ /pubmed/31316654 http://dx.doi.org/10.3889/oamjms.2019.237 Text en Copyright: © 2019 Dragica Zendelovska, Emilija Atanasovska, Kalina Gjorgjievska, Kristina Pavlovska, Krume Jakjovski, Dimce Zafirov, Jasmina Trojacanec. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). |
spellingShingle | Basic Science Zendelovska, Dragica Atanasovska, Emilija Gjorgjievska, Kalina Pavlovska, Kristina Jakjovski, Krume Zafirov, Dimce Trojacanec, Jasmina A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography |
title | A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography |
title_full | A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography |
title_fullStr | A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography |
title_full_unstemmed | A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography |
title_short | A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography |
title_sort | new solid-phase extraction method for determination of pantoprazole in human plasma using high-performance liquid chromatography |
topic | Basic Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614261/ https://www.ncbi.nlm.nih.gov/pubmed/31316654 http://dx.doi.org/10.3889/oamjms.2019.237 |
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