Cargando…

Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers

We have adapted a non-invasive method based on optical tweezers technology to differentiate between the normal B-cells and the B-cell non-Hodgkin lymphoma (B-NHL) cells derived from clinical samples. Our approach bases on the nascent adhesion between an individual B-cell and a mesenchymal stromal ce...

Descripción completa

Detalles Bibliográficos
Autores principales: Duś-Szachniewicz, Kamila, Drobczyński, Sławomir, Woźniak, Marta, Zduniak, Krzysztof, Ostasiewicz, Katarzyna, Ziółkowski, Piotr, Korzeniewska, Aleksandra K., Agrawal, Anil K., Kołodziej, Paweł, Walaszek, Kinga, Bystydzieński, Zbigniew, Rymkiewicz, Grzegorz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614388/
https://www.ncbi.nlm.nih.gov/pubmed/31285461
http://dx.doi.org/10.1038/s41598-019-46086-y
_version_ 1783433172651868160
author Duś-Szachniewicz, Kamila
Drobczyński, Sławomir
Woźniak, Marta
Zduniak, Krzysztof
Ostasiewicz, Katarzyna
Ziółkowski, Piotr
Korzeniewska, Aleksandra K.
Agrawal, Anil K.
Kołodziej, Paweł
Walaszek, Kinga
Bystydzieński, Zbigniew
Rymkiewicz, Grzegorz
author_facet Duś-Szachniewicz, Kamila
Drobczyński, Sławomir
Woźniak, Marta
Zduniak, Krzysztof
Ostasiewicz, Katarzyna
Ziółkowski, Piotr
Korzeniewska, Aleksandra K.
Agrawal, Anil K.
Kołodziej, Paweł
Walaszek, Kinga
Bystydzieński, Zbigniew
Rymkiewicz, Grzegorz
author_sort Duś-Szachniewicz, Kamila
collection PubMed
description We have adapted a non-invasive method based on optical tweezers technology to differentiate between the normal B-cells and the B-cell non-Hodgkin lymphoma (B-NHL) cells derived from clinical samples. Our approach bases on the nascent adhesion between an individual B-cell and a mesenchymal stromal cell. In this study, a single B-cell was trapped and optically seeded on a mesenchymal stromal cell and kept in a direct contact with it until a stable connection between the cells was formed in time scale. This approach allowed us to avoid the introduction of any exogenous beads or chemicals into the experimental setup which would have affected the cell-to-cell adhesion. Here, we have provided new evidence that aberrant adhesive properties found in transformed B-cells are related to malignant neoplasia. We have demonstrated that the mean time required for establishing adhesive interactions between an individual normal B-cell and a mesenchymal stromal cell was 26.7 ± 16.6 s, while for lymphoma cell it was 208.8 ± 102.3 s, p < 0.001. The contact time for adhesion to occur ranged from 5 to 90 s and from 60 to 480 s for normal B-cells and lymphoma cells, respectively. This method for optically controlled cell-to-cell adhesion in time scale is beneficial to the successful differentiation of pathological cells from normal B-cells within the fine needle aspiration biopsy of a clinical sample. Additionally, variations in time-dependent adhesion among subtypes of B-NHL, established here by the optical trapping, confirm earlier results pertaining to cell heterogeneity.
format Online
Article
Text
id pubmed-6614388
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-66143882019-07-17 Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers Duś-Szachniewicz, Kamila Drobczyński, Sławomir Woźniak, Marta Zduniak, Krzysztof Ostasiewicz, Katarzyna Ziółkowski, Piotr Korzeniewska, Aleksandra K. Agrawal, Anil K. Kołodziej, Paweł Walaszek, Kinga Bystydzieński, Zbigniew Rymkiewicz, Grzegorz Sci Rep Article We have adapted a non-invasive method based on optical tweezers technology to differentiate between the normal B-cells and the B-cell non-Hodgkin lymphoma (B-NHL) cells derived from clinical samples. Our approach bases on the nascent adhesion between an individual B-cell and a mesenchymal stromal cell. In this study, a single B-cell was trapped and optically seeded on a mesenchymal stromal cell and kept in a direct contact with it until a stable connection between the cells was formed in time scale. This approach allowed us to avoid the introduction of any exogenous beads or chemicals into the experimental setup which would have affected the cell-to-cell adhesion. Here, we have provided new evidence that aberrant adhesive properties found in transformed B-cells are related to malignant neoplasia. We have demonstrated that the mean time required for establishing adhesive interactions between an individual normal B-cell and a mesenchymal stromal cell was 26.7 ± 16.6 s, while for lymphoma cell it was 208.8 ± 102.3 s, p < 0.001. The contact time for adhesion to occur ranged from 5 to 90 s and from 60 to 480 s for normal B-cells and lymphoma cells, respectively. This method for optically controlled cell-to-cell adhesion in time scale is beneficial to the successful differentiation of pathological cells from normal B-cells within the fine needle aspiration biopsy of a clinical sample. Additionally, variations in time-dependent adhesion among subtypes of B-NHL, established here by the optical trapping, confirm earlier results pertaining to cell heterogeneity. Nature Publishing Group UK 2019-07-08 /pmc/articles/PMC6614388/ /pubmed/31285461 http://dx.doi.org/10.1038/s41598-019-46086-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Duś-Szachniewicz, Kamila
Drobczyński, Sławomir
Woźniak, Marta
Zduniak, Krzysztof
Ostasiewicz, Katarzyna
Ziółkowski, Piotr
Korzeniewska, Aleksandra K.
Agrawal, Anil K.
Kołodziej, Paweł
Walaszek, Kinga
Bystydzieński, Zbigniew
Rymkiewicz, Grzegorz
Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers
title Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers
title_full Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers
title_fullStr Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers
title_full_unstemmed Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers
title_short Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers
title_sort differentiation of single lymphoma primary cells and normal b-cells based on their adhesion to mesenchymal stromal cells in optical tweezers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614388/
https://www.ncbi.nlm.nih.gov/pubmed/31285461
http://dx.doi.org/10.1038/s41598-019-46086-y
work_keys_str_mv AT dusszachniewiczkamila differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT drobczynskisławomir differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT wozniakmarta differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT zduniakkrzysztof differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT ostasiewiczkatarzyna differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT ziołkowskipiotr differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT korzeniewskaaleksandrak differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT agrawalanilk differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT kołodziejpaweł differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT walaszekkinga differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT bystydzienskizbigniew differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers
AT rymkiewiczgrzegorz differentiationofsinglelymphomaprimarycellsandnormalbcellsbasedontheiradhesiontomesenchymalstromalcellsinopticaltweezers