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BODIPY-cholesterol can be reliably used to monitor cholesterol efflux from capacitating mammalian spermatozoa
Capacitation is the final maturation step spermatozoa undergo prior to fertilisation. The efflux of cholesterol from the sperm membrane to the extracellular environment is a crucial step during capacitation but current methods to quantify this process are suboptimal. In this study, we validate the u...
Autores principales: | Bernecic, N. C., Zhang, M., Gadella, B. M., Brouwers, J. F. H. M., Jansen, J. W. A., Arkesteijn, G. J. A., de Graaf, S. P., Leahy, T. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614389/ https://www.ncbi.nlm.nih.gov/pubmed/31285440 http://dx.doi.org/10.1038/s41598-019-45831-7 |
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