MicroRNA-212-3p inhibits the Proliferation and Invasion of Human Hepatocellular Carcinoma Cells by Suppressing CTGF expression

MicroRNA-212-3p inhibits several human cancers but its effects on hepatocellular carcinoma (HCC) remain unclear. In this study, we show that miR-212-3p is down-regulated in HCC cell lines and tissues, and correlates with vascular invasion (p = 0.001), and the absence of capsule formation (p = 0.009)...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Jian-qing, Ou, Yang-liu, Huang, Zhi-ping, Hong, Yong-gang, Tao, Yuan-ping, Wang, Zhen-guang, Ni, Jun-sheng, Hao, Li-qiang, Lin, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614456/
https://www.ncbi.nlm.nih.gov/pubmed/31285444
http://dx.doi.org/10.1038/s41598-019-46088-w
_version_ 1783433188539891712
author Chen, Jian-qing
Ou, Yang-liu
Huang, Zhi-ping
Hong, Yong-gang
Tao, Yuan-ping
Wang, Zhen-guang
Ni, Jun-sheng
Hao, Li-qiang
Lin, Hui
author_facet Chen, Jian-qing
Ou, Yang-liu
Huang, Zhi-ping
Hong, Yong-gang
Tao, Yuan-ping
Wang, Zhen-guang
Ni, Jun-sheng
Hao, Li-qiang
Lin, Hui
author_sort Chen, Jian-qing
collection PubMed
description MicroRNA-212-3p inhibits several human cancers but its effects on hepatocellular carcinoma (HCC) remain unclear. In this study, we show that miR-212-3p is down-regulated in HCC cell lines and tissues, and correlates with vascular invasion (p = 0.001), and the absence of capsule formation (p = 0.009). We found that miR-212-3p influenced the epithelial to mesenchymal transition (EMT) of HCCLM3 and Huh7 cells. Mechanistically, miR-212-3p repressed cell invasion through the suppression of connective tissue growth factor (CTGF). We therefore validate the anti-HCC effects of miR-212-3p through its ability to suppress CTGF and subsequent EMT.
format Online
Article
Text
id pubmed-6614456
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-66144562019-07-17 MicroRNA-212-3p inhibits the Proliferation and Invasion of Human Hepatocellular Carcinoma Cells by Suppressing CTGF expression Chen, Jian-qing Ou, Yang-liu Huang, Zhi-ping Hong, Yong-gang Tao, Yuan-ping Wang, Zhen-guang Ni, Jun-sheng Hao, Li-qiang Lin, Hui Sci Rep Article MicroRNA-212-3p inhibits several human cancers but its effects on hepatocellular carcinoma (HCC) remain unclear. In this study, we show that miR-212-3p is down-regulated in HCC cell lines and tissues, and correlates with vascular invasion (p = 0.001), and the absence of capsule formation (p = 0.009). We found that miR-212-3p influenced the epithelial to mesenchymal transition (EMT) of HCCLM3 and Huh7 cells. Mechanistically, miR-212-3p repressed cell invasion through the suppression of connective tissue growth factor (CTGF). We therefore validate the anti-HCC effects of miR-212-3p through its ability to suppress CTGF and subsequent EMT. Nature Publishing Group UK 2019-07-08 /pmc/articles/PMC6614456/ /pubmed/31285444 http://dx.doi.org/10.1038/s41598-019-46088-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chen, Jian-qing
Ou, Yang-liu
Huang, Zhi-ping
Hong, Yong-gang
Tao, Yuan-ping
Wang, Zhen-guang
Ni, Jun-sheng
Hao, Li-qiang
Lin, Hui
MicroRNA-212-3p inhibits the Proliferation and Invasion of Human Hepatocellular Carcinoma Cells by Suppressing CTGF expression
title MicroRNA-212-3p inhibits the Proliferation and Invasion of Human Hepatocellular Carcinoma Cells by Suppressing CTGF expression
title_full MicroRNA-212-3p inhibits the Proliferation and Invasion of Human Hepatocellular Carcinoma Cells by Suppressing CTGF expression
title_fullStr MicroRNA-212-3p inhibits the Proliferation and Invasion of Human Hepatocellular Carcinoma Cells by Suppressing CTGF expression
title_full_unstemmed MicroRNA-212-3p inhibits the Proliferation and Invasion of Human Hepatocellular Carcinoma Cells by Suppressing CTGF expression
title_short MicroRNA-212-3p inhibits the Proliferation and Invasion of Human Hepatocellular Carcinoma Cells by Suppressing CTGF expression
title_sort microrna-212-3p inhibits the proliferation and invasion of human hepatocellular carcinoma cells by suppressing ctgf expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614456/
https://www.ncbi.nlm.nih.gov/pubmed/31285444
http://dx.doi.org/10.1038/s41598-019-46088-w
work_keys_str_mv AT chenjianqing microrna2123pinhibitstheproliferationandinvasionofhumanhepatocellularcarcinomacellsbysuppressingctgfexpression
AT ouyangliu microrna2123pinhibitstheproliferationandinvasionofhumanhepatocellularcarcinomacellsbysuppressingctgfexpression
AT huangzhiping microrna2123pinhibitstheproliferationandinvasionofhumanhepatocellularcarcinomacellsbysuppressingctgfexpression
AT hongyonggang microrna2123pinhibitstheproliferationandinvasionofhumanhepatocellularcarcinomacellsbysuppressingctgfexpression
AT taoyuanping microrna2123pinhibitstheproliferationandinvasionofhumanhepatocellularcarcinomacellsbysuppressingctgfexpression
AT wangzhenguang microrna2123pinhibitstheproliferationandinvasionofhumanhepatocellularcarcinomacellsbysuppressingctgfexpression
AT nijunsheng microrna2123pinhibitstheproliferationandinvasionofhumanhepatocellularcarcinomacellsbysuppressingctgfexpression
AT haoliqiang microrna2123pinhibitstheproliferationandinvasionofhumanhepatocellularcarcinomacellsbysuppressingctgfexpression
AT linhui microrna2123pinhibitstheproliferationandinvasionofhumanhepatocellularcarcinomacellsbysuppressingctgfexpression

Ejemplares similares