Cargando…
CDS-1548 induces apoptosis in HeLa cells by activating caspase 3
Cervical cancer continues to be a threat to female health globally. In the present study, the potential anticancer activity of 2-[2-hydroxyl-1-(4-methoxy phenyl) ethyl]-3-(4-benzyloxy phenyl) isoindolin-1-one (CDS-1548), was evaluated in HeLa cells. CDS-1548 is an organic small-molecule compound cha...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614674/ https://www.ncbi.nlm.nih.gov/pubmed/31423257 http://dx.doi.org/10.3892/ol.2019.10511 |
_version_ | 1783433223001341952 |
---|---|
author | Zhang, Yuanxin Ge, Yakun Shi, Wei Hu, Chenming Bai, Xu Rong, Jimin Yu, Ming |
author_facet | Zhang, Yuanxin Ge, Yakun Shi, Wei Hu, Chenming Bai, Xu Rong, Jimin Yu, Ming |
author_sort | Zhang, Yuanxin |
collection | PubMed |
description | Cervical cancer continues to be a threat to female health globally. In the present study, the potential anticancer activity of 2-[2-hydroxyl-1-(4-methoxy phenyl) ethyl]-3-(4-benzyloxy phenyl) isoindolin-1-one (CDS-1548), was evaluated in HeLa cells. CDS-1548 is an organic small-molecule compound characterized by two chiral centers, with the nuclear parent 1H-isoindolin-1-one. CDS-1548 administration significantly elevated the transcriptional activity of p53 and its downstream target genes in a dose-dependent manner. Additionally, CDS-1548 treatment increased the expression levels of p53 and mouse double minute 2 homolog, as well as inducing apoptosis in HeLa cells. Furthermore, CDS-1548 treatment downregulated the expression of B-cell lymphoma 2, upregulated Bcl-2 homologous antagonist killer, promoted the release of cytochrome c from mitochondria to cytoplasm, and activated the production of caspase 3 and 9. Collectively, these results suggested that CDS-1548 inhibited HeLa cell proliferation by promoting G(2)/M cell cycle phase arrest and inducting of mitochondria-mediated apoptosis. |
format | Online Article Text |
id | pubmed-6614674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-66146742019-08-18 CDS-1548 induces apoptosis in HeLa cells by activating caspase 3 Zhang, Yuanxin Ge, Yakun Shi, Wei Hu, Chenming Bai, Xu Rong, Jimin Yu, Ming Oncol Lett Articles Cervical cancer continues to be a threat to female health globally. In the present study, the potential anticancer activity of 2-[2-hydroxyl-1-(4-methoxy phenyl) ethyl]-3-(4-benzyloxy phenyl) isoindolin-1-one (CDS-1548), was evaluated in HeLa cells. CDS-1548 is an organic small-molecule compound characterized by two chiral centers, with the nuclear parent 1H-isoindolin-1-one. CDS-1548 administration significantly elevated the transcriptional activity of p53 and its downstream target genes in a dose-dependent manner. Additionally, CDS-1548 treatment increased the expression levels of p53 and mouse double minute 2 homolog, as well as inducing apoptosis in HeLa cells. Furthermore, CDS-1548 treatment downregulated the expression of B-cell lymphoma 2, upregulated Bcl-2 homologous antagonist killer, promoted the release of cytochrome c from mitochondria to cytoplasm, and activated the production of caspase 3 and 9. Collectively, these results suggested that CDS-1548 inhibited HeLa cell proliferation by promoting G(2)/M cell cycle phase arrest and inducting of mitochondria-mediated apoptosis. D.A. Spandidos 2019-08 2019-06-21 /pmc/articles/PMC6614674/ /pubmed/31423257 http://dx.doi.org/10.3892/ol.2019.10511 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Yuanxin Ge, Yakun Shi, Wei Hu, Chenming Bai, Xu Rong, Jimin Yu, Ming CDS-1548 induces apoptosis in HeLa cells by activating caspase 3 |
title | CDS-1548 induces apoptosis in HeLa cells by activating caspase 3 |
title_full | CDS-1548 induces apoptosis in HeLa cells by activating caspase 3 |
title_fullStr | CDS-1548 induces apoptosis in HeLa cells by activating caspase 3 |
title_full_unstemmed | CDS-1548 induces apoptosis in HeLa cells by activating caspase 3 |
title_short | CDS-1548 induces apoptosis in HeLa cells by activating caspase 3 |
title_sort | cds-1548 induces apoptosis in hela cells by activating caspase 3 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614674/ https://www.ncbi.nlm.nih.gov/pubmed/31423257 http://dx.doi.org/10.3892/ol.2019.10511 |
work_keys_str_mv | AT zhangyuanxin cds1548inducesapoptosisinhelacellsbyactivatingcaspase3 AT geyakun cds1548inducesapoptosisinhelacellsbyactivatingcaspase3 AT shiwei cds1548inducesapoptosisinhelacellsbyactivatingcaspase3 AT huchenming cds1548inducesapoptosisinhelacellsbyactivatingcaspase3 AT baixu cds1548inducesapoptosisinhelacellsbyactivatingcaspase3 AT rongjimin cds1548inducesapoptosisinhelacellsbyactivatingcaspase3 AT yuming cds1548inducesapoptosisinhelacellsbyactivatingcaspase3 |