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Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis
Ribosomal RNA synthesis in Escherichia coli involves a transcription complex, in which RNA polymerase is modified by a signal element on the transcript, Nus factors A, B, E and G, ribosomal protein S4 and inositol mono-phosphatase SuhB. This complex is resistant to ρ-dependent termination and facili...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614801/ https://www.ncbi.nlm.nih.gov/pubmed/31020314 http://dx.doi.org/10.1093/nar/gkz290 |
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author | Huang, Yong-Heng Said, Nelly Loll, Bernhard Wahl, Markus C |
author_facet | Huang, Yong-Heng Said, Nelly Loll, Bernhard Wahl, Markus C |
author_sort | Huang, Yong-Heng |
collection | PubMed |
description | Ribosomal RNA synthesis in Escherichia coli involves a transcription complex, in which RNA polymerase is modified by a signal element on the transcript, Nus factors A, B, E and G, ribosomal protein S4 and inositol mono-phosphatase SuhB. This complex is resistant to ρ-dependent termination and facilitates ribosomal RNA folding, maturation and subunit assembly. The functional contributions of SuhB and their structural bases are presently unclear. We show that SuhB directly binds the RNA signal element and the C-terminal AR2 domain of NusA, and we delineate the atomic basis of the latter interaction by macromolecular crystallography. SuhB recruitment to a ribosomal RNA transcription complex depends on the RNA signal element but not on the NusA AR2 domain. SuhB in turn is required for stable integration of the NusB/E dimer into the complex. In vitro transcription assays revealed that SuhB is crucial for delaying or suppressing ρ-dependent termination, that SuhB also can reduce intrinsic termination, and that SuhB-AR2 contacts contribute to these effects. Together, our results reveal functions of SuhB during ribosomal RNA synthesis and delineate some of the underlying molecular interactions. |
format | Online Article Text |
id | pubmed-6614801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-66148012019-07-12 Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis Huang, Yong-Heng Said, Nelly Loll, Bernhard Wahl, Markus C Nucleic Acids Res Structural Biology Ribosomal RNA synthesis in Escherichia coli involves a transcription complex, in which RNA polymerase is modified by a signal element on the transcript, Nus factors A, B, E and G, ribosomal protein S4 and inositol mono-phosphatase SuhB. This complex is resistant to ρ-dependent termination and facilitates ribosomal RNA folding, maturation and subunit assembly. The functional contributions of SuhB and their structural bases are presently unclear. We show that SuhB directly binds the RNA signal element and the C-terminal AR2 domain of NusA, and we delineate the atomic basis of the latter interaction by macromolecular crystallography. SuhB recruitment to a ribosomal RNA transcription complex depends on the RNA signal element but not on the NusA AR2 domain. SuhB in turn is required for stable integration of the NusB/E dimer into the complex. In vitro transcription assays revealed that SuhB is crucial for delaying or suppressing ρ-dependent termination, that SuhB also can reduce intrinsic termination, and that SuhB-AR2 contacts contribute to these effects. Together, our results reveal functions of SuhB during ribosomal RNA synthesis and delineate some of the underlying molecular interactions. Oxford University Press 2019-07-09 2019-04-25 /pmc/articles/PMC6614801/ /pubmed/31020314 http://dx.doi.org/10.1093/nar/gkz290 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Structural Biology Huang, Yong-Heng Said, Nelly Loll, Bernhard Wahl, Markus C Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis |
title | Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis |
title_full | Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis |
title_fullStr | Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis |
title_full_unstemmed | Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis |
title_short | Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis |
title_sort | structural basis for the function of suhb as a transcription factor in ribosomal rna synthesis |
topic | Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614801/ https://www.ncbi.nlm.nih.gov/pubmed/31020314 http://dx.doi.org/10.1093/nar/gkz290 |
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