MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7

Cardiac hypertrophy mainly predicts heart failure and is highly linked with sudden loss of lives. MicroRNAs (miRNAs) play essential roles in the development of cardiac hypertrophy through binding to corresponding mRNA targets. In this study, in order to investigate the roles of two mature forms of m...

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Autores principales: Wang, Lei, Qin, Dongze, Shi, Hongtao, Zhang, Yanan, Li, Hao, Han, Qinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614993/
https://www.ncbi.nlm.nih.gov/pubmed/31341888
http://dx.doi.org/10.1155/2019/1580982
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author Wang, Lei
Qin, Dongze
Shi, Hongtao
Zhang, Yanan
Li, Hao
Han, Qinghua
author_facet Wang, Lei
Qin, Dongze
Shi, Hongtao
Zhang, Yanan
Li, Hao
Han, Qinghua
author_sort Wang, Lei
collection PubMed
description Cardiac hypertrophy mainly predicts heart failure and is highly linked with sudden loss of lives. MicroRNAs (miRNAs) play essential roles in the development of cardiac hypertrophy through binding to corresponding mRNA targets. In this study, in order to investigate the roles of two mature forms of miRNA-195, miR-195-3p, and miR-195-5p, in vitro and in vivo models of cardiac hypertrophy were established by applying angiotensin II (Ang II) to H9c2 cardiomyocytes and infusing chronic Ang II to mice, respectively. We found that miR-195-5p was evidently equally upregulated in the in vitro and in vivo studies of cardiac hypertrophy induced by Ang II. High expressed miR-195-5p could adequately promote hypertrophy, whereas the suppression of miR-195-5p prevented hypertrophy of H9c2 cardiomyocytes under Ang II treatment. Furthermore, the luciferase reporter system demonstrated that MFN2 and FBWX7 were target genes of miR-195-5p, which negatively regulated the expression of these two genes in H9c2 cells. By contrast, in both models, expression of miR-195-3p was only slightly changed without statistical significance. In addition, we observed a trend towards decreased expression of hypertrophic markers by overexpressing miR-195-3p in AngII-treated H9c2 cardiomyocytes in vitro. Taken together, our study indicates that miR-195-5p promotes cardiac hypertrophy via targeting MFN2 and FBXW7 and may provide promising therapeutic strategies for interfering cardiac hypertrophy.
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spelling pubmed-66149932019-07-24 MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7 Wang, Lei Qin, Dongze Shi, Hongtao Zhang, Yanan Li, Hao Han, Qinghua Biomed Res Int Research Article Cardiac hypertrophy mainly predicts heart failure and is highly linked with sudden loss of lives. MicroRNAs (miRNAs) play essential roles in the development of cardiac hypertrophy through binding to corresponding mRNA targets. In this study, in order to investigate the roles of two mature forms of miRNA-195, miR-195-3p, and miR-195-5p, in vitro and in vivo models of cardiac hypertrophy were established by applying angiotensin II (Ang II) to H9c2 cardiomyocytes and infusing chronic Ang II to mice, respectively. We found that miR-195-5p was evidently equally upregulated in the in vitro and in vivo studies of cardiac hypertrophy induced by Ang II. High expressed miR-195-5p could adequately promote hypertrophy, whereas the suppression of miR-195-5p prevented hypertrophy of H9c2 cardiomyocytes under Ang II treatment. Furthermore, the luciferase reporter system demonstrated that MFN2 and FBWX7 were target genes of miR-195-5p, which negatively regulated the expression of these two genes in H9c2 cells. By contrast, in both models, expression of miR-195-3p was only slightly changed without statistical significance. In addition, we observed a trend towards decreased expression of hypertrophic markers by overexpressing miR-195-3p in AngII-treated H9c2 cardiomyocytes in vitro. Taken together, our study indicates that miR-195-5p promotes cardiac hypertrophy via targeting MFN2 and FBXW7 and may provide promising therapeutic strategies for interfering cardiac hypertrophy. Hindawi 2019-06-25 /pmc/articles/PMC6614993/ /pubmed/31341888 http://dx.doi.org/10.1155/2019/1580982 Text en Copyright © 2019 Lei Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Lei
Qin, Dongze
Shi, Hongtao
Zhang, Yanan
Li, Hao
Han, Qinghua
MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7
title MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7
title_full MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7
title_fullStr MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7
title_full_unstemmed MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7
title_short MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7
title_sort mir-195-5p promotes cardiomyocyte hypertrophy by targeting mfn2 and fbxw7
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614993/
https://www.ncbi.nlm.nih.gov/pubmed/31341888
http://dx.doi.org/10.1155/2019/1580982
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