SMURF-seq: efficient copy number profiling on long-read sequencers

We present SMURF-seq, a protocol to efficiently sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecules. Applying SMURF-seq using the Oxford Nanopore MinION yields up to 30 fragments per read, providing an average of 6.2 and up to 7.5 million mappable f...

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Detalles Bibliográficos
Autores principales: Prabakar, Rishvanth K., Xu, Liya, Hicks, James, Smith, Andrew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6615205/
https://www.ncbi.nlm.nih.gov/pubmed/31287019
http://dx.doi.org/10.1186/s13059-019-1732-1

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