Cargando…

Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses

Yersinia pseudotuberculosis is an important foodborne pathogen threatening modern food safety due to its ability to survive and grow at low temperatures. DEAD-box RNA helicase CsdA has been shown to play an important role in the low-temperature growth of psychrotrophic Y. pseudotuberculosis. A total...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Xiaojie, Keto-Timonen, Riikka, Skurnik, Mikael, Korkeala, Hannu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6615604/
https://www.ncbi.nlm.nih.gov/pubmed/31287844
http://dx.doi.org/10.1371/journal.pone.0219422
_version_ 1783433381159108608
author Jiang, Xiaojie
Keto-Timonen, Riikka
Skurnik, Mikael
Korkeala, Hannu
author_facet Jiang, Xiaojie
Keto-Timonen, Riikka
Skurnik, Mikael
Korkeala, Hannu
author_sort Jiang, Xiaojie
collection PubMed
description Yersinia pseudotuberculosis is an important foodborne pathogen threatening modern food safety due to its ability to survive and grow at low temperatures. DEAD-box RNA helicase CsdA has been shown to play an important role in the low-temperature growth of psychrotrophic Y. pseudotuberculosis. A total of five DEAD-box RNA helicase genes (rhlB, csdA, rhlE, dbpA, srmB) have been identified in Y. pseudotuberculosis IP32953. However, their role in various stress conditions used in food production is unclear. We studied the involvement of the DEAD-box RNA helicase-encoding genes in the cold tolerance of Y. pseudotuberculosis IP32953 using quantitative real-time reverse transcription (RT-qPCR) and mutational analysis. Quantitative RT-PCR revealed that mRNA transcriptional levels of csdA, rhlE, dbpA and srmB were significantly higher after cold shock at 3°C compared to non-shocked culture at 28°C, suggesting the involvement of these four genes in cold shock response at the transcriptional level. The deletion of csdA ceased growth, while the deletion of dbpA or srmB significantly impaired growth at 3°C, suggesting the requirement of these three genes in Y. pseudotuberculosis at low temperatures. Growth of each DEAD-box RNA helicase mutant was also investigated under pH, osmotic, ethanol and oxidative stress conditions. The five helicase-encoding genes did not play major roles in the growth of Y. pseudotuberculosis IP32953 under pH, osmotic, ethanol or oxidative stress.
format Online
Article
Text
id pubmed-6615604
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-66156042019-07-25 Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses Jiang, Xiaojie Keto-Timonen, Riikka Skurnik, Mikael Korkeala, Hannu PLoS One Research Article Yersinia pseudotuberculosis is an important foodborne pathogen threatening modern food safety due to its ability to survive and grow at low temperatures. DEAD-box RNA helicase CsdA has been shown to play an important role in the low-temperature growth of psychrotrophic Y. pseudotuberculosis. A total of five DEAD-box RNA helicase genes (rhlB, csdA, rhlE, dbpA, srmB) have been identified in Y. pseudotuberculosis IP32953. However, their role in various stress conditions used in food production is unclear. We studied the involvement of the DEAD-box RNA helicase-encoding genes in the cold tolerance of Y. pseudotuberculosis IP32953 using quantitative real-time reverse transcription (RT-qPCR) and mutational analysis. Quantitative RT-PCR revealed that mRNA transcriptional levels of csdA, rhlE, dbpA and srmB were significantly higher after cold shock at 3°C compared to non-shocked culture at 28°C, suggesting the involvement of these four genes in cold shock response at the transcriptional level. The deletion of csdA ceased growth, while the deletion of dbpA or srmB significantly impaired growth at 3°C, suggesting the requirement of these three genes in Y. pseudotuberculosis at low temperatures. Growth of each DEAD-box RNA helicase mutant was also investigated under pH, osmotic, ethanol and oxidative stress conditions. The five helicase-encoding genes did not play major roles in the growth of Y. pseudotuberculosis IP32953 under pH, osmotic, ethanol or oxidative stress. Public Library of Science 2019-07-09 /pmc/articles/PMC6615604/ /pubmed/31287844 http://dx.doi.org/10.1371/journal.pone.0219422 Text en © 2019 Jiang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jiang, Xiaojie
Keto-Timonen, Riikka
Skurnik, Mikael
Korkeala, Hannu
Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses
title Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses
title_full Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses
title_fullStr Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses
title_full_unstemmed Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses
title_short Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses
title_sort role of dead-box rna helicase genes in the growth of yersinia pseudotuberculosis ip32953 under cold, ph, osmotic, ethanol and oxidative stresses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6615604/
https://www.ncbi.nlm.nih.gov/pubmed/31287844
http://dx.doi.org/10.1371/journal.pone.0219422
work_keys_str_mv AT jiangxiaojie roleofdeadboxrnahelicasegenesinthegrowthofyersiniapseudotuberculosisip32953undercoldphosmoticethanolandoxidativestresses
AT ketotimonenriikka roleofdeadboxrnahelicasegenesinthegrowthofyersiniapseudotuberculosisip32953undercoldphosmoticethanolandoxidativestresses
AT skurnikmikael roleofdeadboxrnahelicasegenesinthegrowthofyersiniapseudotuberculosisip32953undercoldphosmoticethanolandoxidativestresses
AT korkealahannu roleofdeadboxrnahelicasegenesinthegrowthofyersiniapseudotuberculosisip32953undercoldphosmoticethanolandoxidativestresses