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Intranigral Injection of Endotoxin Suppresses Proliferation of Hippocampal Progenitor Cells
Brain inflammation can result in functional disorders observed in several neurodegenerative diseases and that can be also associated with reduced neurogenesis. In this study, we investigate the effect of mild inflammation, induced by unilateral injection of Endotoxin (ET) in the substantia nigra (SN...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616074/ https://www.ncbi.nlm.nih.gov/pubmed/31333405 http://dx.doi.org/10.3389/fnins.2019.00687 |
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author | Darwish, Batoul Chamaa, Farah Al-Chaer, Elie D. Saadé, Nayef E. Abou-Kheir, Wassim |
author_facet | Darwish, Batoul Chamaa, Farah Al-Chaer, Elie D. Saadé, Nayef E. Abou-Kheir, Wassim |
author_sort | Darwish, Batoul |
collection | PubMed |
description | Brain inflammation can result in functional disorders observed in several neurodegenerative diseases and that can be also associated with reduced neurogenesis. In this study, we investigate the effect of mild inflammation, induced by unilateral injection of Endotoxin (ET) in the substantia nigra (SN)/Ventral Tegmental Area, on the proliferation and survival of stem/progenitor cells in the dentate gyrus (DG) of the hippocampus. Adult female rats received unilateral injection of ET (2 μg/2 μl saline) or sterile saline (2 μl) in the right SN followed by 5′-Bromo-2′-deoxyuridine (BrdU) injections (66 mg/kg/injection). Intranigral ET injection induced bilateral decrease in the number of newly born BrdU positive cells in the DG. This effect was paralleled by a significant decrease in the exploratory behavior of rats, as assessed by the Y-maze novel arm exploration task. ET also induced a transient decrease in the number of tyrosine hydroxylase-positive cells in the injected SN, impaired motor behavior, and caused microglial activation in the SN. This study provides an experimental simulation of the remote effects of moderate and reversible neuroinflammation resulting in impaired communication between midbrain dopaminergic neurons and the hippocampus. |
format | Online Article Text |
id | pubmed-6616074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66160742019-07-22 Intranigral Injection of Endotoxin Suppresses Proliferation of Hippocampal Progenitor Cells Darwish, Batoul Chamaa, Farah Al-Chaer, Elie D. Saadé, Nayef E. Abou-Kheir, Wassim Front Neurosci Neuroscience Brain inflammation can result in functional disorders observed in several neurodegenerative diseases and that can be also associated with reduced neurogenesis. In this study, we investigate the effect of mild inflammation, induced by unilateral injection of Endotoxin (ET) in the substantia nigra (SN)/Ventral Tegmental Area, on the proliferation and survival of stem/progenitor cells in the dentate gyrus (DG) of the hippocampus. Adult female rats received unilateral injection of ET (2 μg/2 μl saline) or sterile saline (2 μl) in the right SN followed by 5′-Bromo-2′-deoxyuridine (BrdU) injections (66 mg/kg/injection). Intranigral ET injection induced bilateral decrease in the number of newly born BrdU positive cells in the DG. This effect was paralleled by a significant decrease in the exploratory behavior of rats, as assessed by the Y-maze novel arm exploration task. ET also induced a transient decrease in the number of tyrosine hydroxylase-positive cells in the injected SN, impaired motor behavior, and caused microglial activation in the SN. This study provides an experimental simulation of the remote effects of moderate and reversible neuroinflammation resulting in impaired communication between midbrain dopaminergic neurons and the hippocampus. Frontiers Media S.A. 2019-07-03 /pmc/articles/PMC6616074/ /pubmed/31333405 http://dx.doi.org/10.3389/fnins.2019.00687 Text en Copyright © 2019 Darwish, Chamaa, Al-Chaer, Saadé and Abou-Kheir. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Darwish, Batoul Chamaa, Farah Al-Chaer, Elie D. Saadé, Nayef E. Abou-Kheir, Wassim Intranigral Injection of Endotoxin Suppresses Proliferation of Hippocampal Progenitor Cells |
title | Intranigral Injection of Endotoxin Suppresses Proliferation of Hippocampal Progenitor Cells |
title_full | Intranigral Injection of Endotoxin Suppresses Proliferation of Hippocampal Progenitor Cells |
title_fullStr | Intranigral Injection of Endotoxin Suppresses Proliferation of Hippocampal Progenitor Cells |
title_full_unstemmed | Intranigral Injection of Endotoxin Suppresses Proliferation of Hippocampal Progenitor Cells |
title_short | Intranigral Injection of Endotoxin Suppresses Proliferation of Hippocampal Progenitor Cells |
title_sort | intranigral injection of endotoxin suppresses proliferation of hippocampal progenitor cells |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616074/ https://www.ncbi.nlm.nih.gov/pubmed/31333405 http://dx.doi.org/10.3389/fnins.2019.00687 |
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