Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China

Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97–100% similarity to the...

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Autores principales: He, Li, Fang, Zheng-Ming, Xue, Ting, Zhang, Er-Fu, An, Chun-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2019
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616165/
https://www.ncbi.nlm.nih.gov/pubmed/31284356
http://dx.doi.org/10.3347/kjp.2019.57.3.309
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author He, Li
Fang, Zheng-Ming
Xue, Ting
Zhang, Er-Fu
An, Chun-Li
author_facet He, Li
Fang, Zheng-Ming
Xue, Ting
Zhang, Er-Fu
An, Chun-Li
author_sort He, Li
collection PubMed
description Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97–100% similarity to the reference sequence of S. erinaceieuropaei and 88–89% to the reference sequence of S. decipiens. There were 1–12 bases different between these worms, but no obvious genetic variation (0–3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1–3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces.
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spelling pubmed-66161652019-07-24 Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China He, Li Fang, Zheng-Ming Xue, Ting Zhang, Er-Fu An, Chun-Li Korean J Parasitol Brief Communication Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97–100% similarity to the reference sequence of S. erinaceieuropaei and 88–89% to the reference sequence of S. decipiens. There were 1–12 bases different between these worms, but no obvious genetic variation (0–3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1–3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces. The Korean Society for Parasitology and Tropical Medicine 2019-06 2019-06-30 /pmc/articles/PMC6616165/ /pubmed/31284356 http://dx.doi.org/10.3347/kjp.2019.57.3.309 Text en Copyright © 2019 by The Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
He, Li
Fang, Zheng-Ming
Xue, Ting
Zhang, Er-Fu
An, Chun-Li
Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China
title Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China
title_full Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China
title_fullStr Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China
title_full_unstemmed Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China
title_short Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China
title_sort genetic identification of spirometra erinaceieuropaei spargana in liaoning and hubei provinces, pr china
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616165/
https://www.ncbi.nlm.nih.gov/pubmed/31284356
http://dx.doi.org/10.3347/kjp.2019.57.3.309
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