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Investigation of silver nanoparticles on titanium surface created by ion implantation technology
Objectives: Using dental Ti implants has become a well-accepted and used method for replacing missing dentition. It has become evident that in many cases peri-implant inflammation develops. The objective was to create and evaluate the antibacterial effect of silver nanoparticle (Ag-NP) coated Ti sur...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616303/ https://www.ncbi.nlm.nih.gov/pubmed/31308654 http://dx.doi.org/10.2147/IJN.S197782 |
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author | Lampé, István Beke, Dezső Biri, Sándor Csarnovics, István Csik, Attila Dombrádi, Zsuzsanna Hajdu, Péter Hegedűs, Viktória Rácz, Richárd Varga, István Hegedűs, Csaba |
author_facet | Lampé, István Beke, Dezső Biri, Sándor Csarnovics, István Csik, Attila Dombrádi, Zsuzsanna Hajdu, Péter Hegedűs, Viktória Rácz, Richárd Varga, István Hegedűs, Csaba |
author_sort | Lampé, István |
collection | PubMed |
description | Objectives: Using dental Ti implants has become a well-accepted and used method for replacing missing dentition. It has become evident that in many cases peri-implant inflammation develops. The objective was to create and evaluate the antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can help to prevent such processes if applied on the surface of dental implants. Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1 cm(2) Grade 2 titanium samples. The antibacterial effect was evaluated by culturing Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and comparing the results to that of glass as control and of pure titanium samples. Alamar Blue assay was carried out to check cytotoxicity. Results: It was proved that silver nanoparticles were present on the treated surfaces. The average diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian distribution, the the filling factor was 25%. Antibacterial evaluation revealed that the nanoparticle covered samples had an antibacterial effect of 64.6% that was statistically significant. Tests also proved that the nanoparticles are safely anchored to the titanium surface and are not cytotoxic. Conclusion: Creating a silver nanoparticle layer can be an option to add antibacterial features to the implant surface and to help in the prevention of peri-implant inflammatory processes. Recent studies demonstrated that silver nanoparticles can induce pathology in mammal cells, thus safe fixation of the particles is essential to prevent them from getting into the circulation. |
format | Online Article Text |
id | pubmed-6616303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-66163032019-07-15 Investigation of silver nanoparticles on titanium surface created by ion implantation technology Lampé, István Beke, Dezső Biri, Sándor Csarnovics, István Csik, Attila Dombrádi, Zsuzsanna Hajdu, Péter Hegedűs, Viktória Rácz, Richárd Varga, István Hegedűs, Csaba Int J Nanomedicine Original Research Objectives: Using dental Ti implants has become a well-accepted and used method for replacing missing dentition. It has become evident that in many cases peri-implant inflammation develops. The objective was to create and evaluate the antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can help to prevent such processes if applied on the surface of dental implants. Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1 cm(2) Grade 2 titanium samples. The antibacterial effect was evaluated by culturing Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and comparing the results to that of glass as control and of pure titanium samples. Alamar Blue assay was carried out to check cytotoxicity. Results: It was proved that silver nanoparticles were present on the treated surfaces. The average diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian distribution, the the filling factor was 25%. Antibacterial evaluation revealed that the nanoparticle covered samples had an antibacterial effect of 64.6% that was statistically significant. Tests also proved that the nanoparticles are safely anchored to the titanium surface and are not cytotoxic. Conclusion: Creating a silver nanoparticle layer can be an option to add antibacterial features to the implant surface and to help in the prevention of peri-implant inflammatory processes. Recent studies demonstrated that silver nanoparticles can induce pathology in mammal cells, thus safe fixation of the particles is essential to prevent them from getting into the circulation. Dove 2019-07-01 /pmc/articles/PMC6616303/ /pubmed/31308654 http://dx.doi.org/10.2147/IJN.S197782 Text en © 2019 Lampé et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Lampé, István Beke, Dezső Biri, Sándor Csarnovics, István Csik, Attila Dombrádi, Zsuzsanna Hajdu, Péter Hegedűs, Viktória Rácz, Richárd Varga, István Hegedűs, Csaba Investigation of silver nanoparticles on titanium surface created by ion implantation technology |
title | Investigation of silver nanoparticles on titanium surface created by ion implantation technology |
title_full | Investigation of silver nanoparticles on titanium surface created by ion implantation technology |
title_fullStr | Investigation of silver nanoparticles on titanium surface created by ion implantation technology |
title_full_unstemmed | Investigation of silver nanoparticles on titanium surface created by ion implantation technology |
title_short | Investigation of silver nanoparticles on titanium surface created by ion implantation technology |
title_sort | investigation of silver nanoparticles on titanium surface created by ion implantation technology |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616303/ https://www.ncbi.nlm.nih.gov/pubmed/31308654 http://dx.doi.org/10.2147/IJN.S197782 |
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