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Does the endometrial cavity have a molecular microbial signature?

Recent molecular studies concluded that the endometrium has a resident microbiota dominated by Lactobacillus spp. and is therefore similar to that of the vagina. These findings were largely derived from endometrial samples obtained through a transcervical catheter and thus prone to contamination. He...

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Autores principales: Winters, Andrew D., Romero, Roberto, Gervasi, Maria Teresa, Gomez-Lopez, Nardhy, Tran, Maria Rosa, Garcia-Flores, Valeria, Pacora, Percy, Jung, Eunjung, Hassan, Sonia S., Hsu, Chaur-Dong, Theis, Kevin R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616349/
https://www.ncbi.nlm.nih.gov/pubmed/31289304
http://dx.doi.org/10.1038/s41598-019-46173-0
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author Winters, Andrew D.
Romero, Roberto
Gervasi, Maria Teresa
Gomez-Lopez, Nardhy
Tran, Maria Rosa
Garcia-Flores, Valeria
Pacora, Percy
Jung, Eunjung
Hassan, Sonia S.
Hsu, Chaur-Dong
Theis, Kevin R.
author_facet Winters, Andrew D.
Romero, Roberto
Gervasi, Maria Teresa
Gomez-Lopez, Nardhy
Tran, Maria Rosa
Garcia-Flores, Valeria
Pacora, Percy
Jung, Eunjung
Hassan, Sonia S.
Hsu, Chaur-Dong
Theis, Kevin R.
author_sort Winters, Andrew D.
collection PubMed
description Recent molecular studies concluded that the endometrium has a resident microbiota dominated by Lactobacillus spp. and is therefore similar to that of the vagina. These findings were largely derived from endometrial samples obtained through a transcervical catheter and thus prone to contamination. Herein, we investigated the molecular microbial profiles of mid-endometrial samples obtained through hysterectomy and compared them with those of the cervix, vagina, rectum, oral cavity, and controls for background DNA contamination. Microbial profiles were examined through 16S rRNA gene qPCR and sequencing. Universal bacterial qPCR of total 16S rDNA revealed a bacterial load exceeding that of background DNA controls in the endometrium of 60% (15/25) of the study subjects. Bacterial profiles of the endometrium differed from those of the oral cavity, rectum, vagina, and background DNA controls, but not of the cervix. The bacterial profiles of the endometrium and cervix were dominated by Acinetobacter, Pseudomonas, Cloacibacterium, and Comamonadaceae. Both 16S rRNA gene sequencing and Lactobacillus species-specific (L. iners & L crispatus) qPCR showed that Lactobacillus was rare in the endometrium. In conclusion, if there is a microbiota in the middle endometrium, it is not dominated by Lactobacillus as was previously concluded, yet further investigation using culture and microscopy is necessary.
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spelling pubmed-66163492019-07-18 Does the endometrial cavity have a molecular microbial signature? Winters, Andrew D. Romero, Roberto Gervasi, Maria Teresa Gomez-Lopez, Nardhy Tran, Maria Rosa Garcia-Flores, Valeria Pacora, Percy Jung, Eunjung Hassan, Sonia S. Hsu, Chaur-Dong Theis, Kevin R. Sci Rep Article Recent molecular studies concluded that the endometrium has a resident microbiota dominated by Lactobacillus spp. and is therefore similar to that of the vagina. These findings were largely derived from endometrial samples obtained through a transcervical catheter and thus prone to contamination. Herein, we investigated the molecular microbial profiles of mid-endometrial samples obtained through hysterectomy and compared them with those of the cervix, vagina, rectum, oral cavity, and controls for background DNA contamination. Microbial profiles were examined through 16S rRNA gene qPCR and sequencing. Universal bacterial qPCR of total 16S rDNA revealed a bacterial load exceeding that of background DNA controls in the endometrium of 60% (15/25) of the study subjects. Bacterial profiles of the endometrium differed from those of the oral cavity, rectum, vagina, and background DNA controls, but not of the cervix. The bacterial profiles of the endometrium and cervix were dominated by Acinetobacter, Pseudomonas, Cloacibacterium, and Comamonadaceae. Both 16S rRNA gene sequencing and Lactobacillus species-specific (L. iners & L crispatus) qPCR showed that Lactobacillus was rare in the endometrium. In conclusion, if there is a microbiota in the middle endometrium, it is not dominated by Lactobacillus as was previously concluded, yet further investigation using culture and microscopy is necessary. Nature Publishing Group UK 2019-07-09 /pmc/articles/PMC6616349/ /pubmed/31289304 http://dx.doi.org/10.1038/s41598-019-46173-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Winters, Andrew D.
Romero, Roberto
Gervasi, Maria Teresa
Gomez-Lopez, Nardhy
Tran, Maria Rosa
Garcia-Flores, Valeria
Pacora, Percy
Jung, Eunjung
Hassan, Sonia S.
Hsu, Chaur-Dong
Theis, Kevin R.
Does the endometrial cavity have a molecular microbial signature?
title Does the endometrial cavity have a molecular microbial signature?
title_full Does the endometrial cavity have a molecular microbial signature?
title_fullStr Does the endometrial cavity have a molecular microbial signature?
title_full_unstemmed Does the endometrial cavity have a molecular microbial signature?
title_short Does the endometrial cavity have a molecular microbial signature?
title_sort does the endometrial cavity have a molecular microbial signature?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616349/
https://www.ncbi.nlm.nih.gov/pubmed/31289304
http://dx.doi.org/10.1038/s41598-019-46173-0
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