Characterization of Sinomonas gamaensis sp. nov., a Novel Soil Bacterium with Antifungal Activity against Exserohilum turcicum

A novel Gram staining positive, aerobic bacterium NEAU-HV1(T) that exhibits antifungal activity against Exserohilum turcicum was isolated from a soil collected from Gama, Hadjer lamis, Chad. It was grown at 10–45 °C (optimum 30 °C), pH 5–10 (optimum pH 8), and 0–4% (w/v) NaCl (optimum 1%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-HV1(T) was closely related to Sinomonas susongensis A31(T) (99.24% sequence similarity), Sinomonas humi MUSC 117(T) (98.76%), and Sinomonas albida LC13(T) (98.68%). The average nucleotide identity values between NEAU-HV1(T) and its most closely related species were 79.34−85.49%. The digital DNA–DNA hybridization values between NEAU-HV1(T) and S. susongensis A31(T), S. albida LC13(T), and S. humi MUSC 117(T) were 23.20, 23.50, and 22.80%, respectively, again indicating that they belonged to different taxa. The genomic DNA G+C content was 67.64 mol%. The whole cell sugars contained galactose, mannose, and rhamnose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and four glycolipids. The respiratory quinone system comprised MK-9(H(2)), MK-10(H(2)), and MK-8(H(2)). The major cellular fatty acids (>5%) were anteiso-C(15:0), anteiso-C(17:0), C(16:0), and iso-C(15:0). Based on the polyphasic analysis, it is suggested that the strain NEAU-HV1(T) represents a novel species of the genus Sinomonas, for which the name Sinomonas gamaensis sp. nov. is proposed. The type strain is NEAU-HV1(T) (= DSM 104514(T) = CCTCC M 2017246(T)).