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Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging

The inactivation of Escherichia coli O157:H7 (E. coli) in physiological saline and lotus roots by high hydrostatic pressure (HHP) in combination with CO(2) or N(2) was studied. Changes in the morphology, cellular structure, and membrane permeability of the cells in physiological saline after treatme...

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Autores principales: Zhou, Bing, Zhang, Luyao, Wang, Xiao, Dong, Peng, Hu, Xiaosong, Zhang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617376/
https://www.ncbi.nlm.nih.gov/pubmed/31141917
http://dx.doi.org/10.3390/microorganisms7060154
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author Zhou, Bing
Zhang, Luyao
Wang, Xiao
Dong, Peng
Hu, Xiaosong
Zhang, Yan
author_facet Zhou, Bing
Zhang, Luyao
Wang, Xiao
Dong, Peng
Hu, Xiaosong
Zhang, Yan
author_sort Zhou, Bing
collection PubMed
description The inactivation of Escherichia coli O157:H7 (E. coli) in physiological saline and lotus roots by high hydrostatic pressure (HHP) in combination with CO(2) or N(2) was studied. Changes in the morphology, cellular structure, and membrane permeability of the cells in physiological saline after treatments were investigated using scanning electron microscopy, transmission electron microscopy, and flow cytometry, respectively. It was shown that after HHP treatments at 150–550 MPa, CO(2)-packed E. coli cells had higher inactivation than the N(2)-packed and vacuum-packed cells, and no significant difference was observed in the latter two groups. Further, both the morphology and intracellular structure of CO(2)-packed E.coli cells were strongly destroyed by high hydrostatic pressure. However, serious damage to the intracellular structures occurred in only the N(2)-packed E. coli cells. During HHP treatments, the presence of CO(2) caused more disruptions in the membrane of E. coli cells than in the N(2)-packed and vacuum-packed cells. These results indicate that the combined treatment of HHP and CO(2) had a strong synergistic bactericidal effect, whereas N(2) did not have synergistic effects with HHP. Although these two combined treatments had different effects on the inactivation of E. coli cells, the inactivation mechanisms might be similar. During both treatments, E. coli cells were inactivated by cell damage induced to the cellular structure through the membrane components and the extracellular morphology, unlike the independent HHP treatment.
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spelling pubmed-66173762019-07-18 Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging Zhou, Bing Zhang, Luyao Wang, Xiao Dong, Peng Hu, Xiaosong Zhang, Yan Microorganisms Article The inactivation of Escherichia coli O157:H7 (E. coli) in physiological saline and lotus roots by high hydrostatic pressure (HHP) in combination with CO(2) or N(2) was studied. Changes in the morphology, cellular structure, and membrane permeability of the cells in physiological saline after treatments were investigated using scanning electron microscopy, transmission electron microscopy, and flow cytometry, respectively. It was shown that after HHP treatments at 150–550 MPa, CO(2)-packed E. coli cells had higher inactivation than the N(2)-packed and vacuum-packed cells, and no significant difference was observed in the latter two groups. Further, both the morphology and intracellular structure of CO(2)-packed E.coli cells were strongly destroyed by high hydrostatic pressure. However, serious damage to the intracellular structures occurred in only the N(2)-packed E. coli cells. During HHP treatments, the presence of CO(2) caused more disruptions in the membrane of E. coli cells than in the N(2)-packed and vacuum-packed cells. These results indicate that the combined treatment of HHP and CO(2) had a strong synergistic bactericidal effect, whereas N(2) did not have synergistic effects with HHP. Although these two combined treatments had different effects on the inactivation of E. coli cells, the inactivation mechanisms might be similar. During both treatments, E. coli cells were inactivated by cell damage induced to the cellular structure through the membrane components and the extracellular morphology, unlike the independent HHP treatment. MDPI 2019-05-28 /pmc/articles/PMC6617376/ /pubmed/31141917 http://dx.doi.org/10.3390/microorganisms7060154 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Bing
Zhang, Luyao
Wang, Xiao
Dong, Peng
Hu, Xiaosong
Zhang, Yan
Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging
title Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging
title_full Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging
title_fullStr Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging
title_full_unstemmed Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging
title_short Inactivation of Escherichia coli O157:H7 by High Hydrostatic Pressure Combined with Gas Packaging
title_sort inactivation of escherichia coli o157:h7 by high hydrostatic pressure combined with gas packaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617376/
https://www.ncbi.nlm.nih.gov/pubmed/31141917
http://dx.doi.org/10.3390/microorganisms7060154
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