Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish

The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after c...

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Autores principales: Narumanchi, Suneeta, Kalervo, Karri, Perttunen, Sanni, Wang, Hong, Immonen, Katariina, Kosonen, Riikka, Laine, Mika, Ruskoaho, Heikki, Tikkanen, Ilkka, Lakkisto, Päivi, Paavola, Jere
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617397/
https://www.ncbi.nlm.nih.gov/pubmed/30987331
http://dx.doi.org/10.3390/jcdd6020016
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author Narumanchi, Suneeta
Kalervo, Karri
Perttunen, Sanni
Wang, Hong
Immonen, Katariina
Kosonen, Riikka
Laine, Mika
Ruskoaho, Heikki
Tikkanen, Ilkka
Lakkisto, Päivi
Paavola, Jere
author_facet Narumanchi, Suneeta
Kalervo, Karri
Perttunen, Sanni
Wang, Hong
Immonen, Katariina
Kosonen, Riikka
Laine, Mika
Ruskoaho, Heikki
Tikkanen, Ilkka
Lakkisto, Päivi
Paavola, Jere
author_sort Narumanchi, Suneeta
collection PubMed
description The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker raldh2 at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c. These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration.
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spelling pubmed-66173972019-07-18 Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish Narumanchi, Suneeta Kalervo, Karri Perttunen, Sanni Wang, Hong Immonen, Katariina Kosonen, Riikka Laine, Mika Ruskoaho, Heikki Tikkanen, Ilkka Lakkisto, Päivi Paavola, Jere J Cardiovasc Dev Dis Article The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker raldh2 at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c. These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration. MDPI 2019-04-04 /pmc/articles/PMC6617397/ /pubmed/30987331 http://dx.doi.org/10.3390/jcdd6020016 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Narumanchi, Suneeta
Kalervo, Karri
Perttunen, Sanni
Wang, Hong
Immonen, Katariina
Kosonen, Riikka
Laine, Mika
Ruskoaho, Heikki
Tikkanen, Ilkka
Lakkisto, Päivi
Paavola, Jere
Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
title Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
title_full Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
title_fullStr Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
title_full_unstemmed Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
title_short Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
title_sort inhibition of let-7c regulates cardiac regeneration after cryoinjury in adult zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617397/
https://www.ncbi.nlm.nih.gov/pubmed/30987331
http://dx.doi.org/10.3390/jcdd6020016
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