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Exoproteome Heterogeneity among Closely Related Staphylococcus aureus t437 Isolates and Possible Implications for Virulence

[Image: see text] Staphylococcus aureus with spa-type t437 has been identified as a predominant community-associated methicillin-resistant S. aureus clone from Asia, which is also encountered in Europe. Molecular typing has previously shown that t437 isolates are highly similar regardless of geograp...

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Autores principales: Zhao, Xin, Palma Medina, Laura M., Stobernack, Tim, Glasner, Corinna, de Jong, Anne, Utari, Putri, Setroikromo, Rita, Quax, Wim J., Otto, Andreas, Becher, Dörte, Buist, Girbe, van Dijl, Jan Maarten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617432/
https://www.ncbi.nlm.nih.gov/pubmed/31119940
http://dx.doi.org/10.1021/acs.jproteome.9b00179
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author Zhao, Xin
Palma Medina, Laura M.
Stobernack, Tim
Glasner, Corinna
de Jong, Anne
Utari, Putri
Setroikromo, Rita
Quax, Wim J.
Otto, Andreas
Becher, Dörte
Buist, Girbe
van Dijl, Jan Maarten
author_facet Zhao, Xin
Palma Medina, Laura M.
Stobernack, Tim
Glasner, Corinna
de Jong, Anne
Utari, Putri
Setroikromo, Rita
Quax, Wim J.
Otto, Andreas
Becher, Dörte
Buist, Girbe
van Dijl, Jan Maarten
author_sort Zhao, Xin
collection PubMed
description [Image: see text] Staphylococcus aureus with spa-type t437 has been identified as a predominant community-associated methicillin-resistant S. aureus clone from Asia, which is also encountered in Europe. Molecular typing has previously shown that t437 isolates are highly similar regardless of geographical regions or host environments. The present study was aimed at assessing to what extent this high similarity is actually reflected in the production of secreted virulence factors. We therefore profiled the extracellular proteome, representing the main reservoir of virulence factors, of 20 representative clinical isolates by mass spectrometry. The results show that these isolates can be divided into three groups and nine subgroups based on exoproteome abundance signatures. This implies that S. aureus t437 isolates show substantial exoproteome heterogeneity. Nonetheless, 30 highly conserved extracellular proteins, of which about 50% have a predicted role in pathogenesis, were dominantly identified. To approximate the virulence of the 20 investigated isolates, we employed infection models based on Galleria mellonella and HeLa cells. The results show that the grouping of clinical isolates based on their exoproteome profile can be related to virulence. We consider this outcome important as our approach provides a tool to pinpoint differences in virulence among seemingly highly similar clinical isolates of S. aureus.
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spelling pubmed-66174322019-07-11 Exoproteome Heterogeneity among Closely Related Staphylococcus aureus t437 Isolates and Possible Implications for Virulence Zhao, Xin Palma Medina, Laura M. Stobernack, Tim Glasner, Corinna de Jong, Anne Utari, Putri Setroikromo, Rita Quax, Wim J. Otto, Andreas Becher, Dörte Buist, Girbe van Dijl, Jan Maarten J Proteome Res [Image: see text] Staphylococcus aureus with spa-type t437 has been identified as a predominant community-associated methicillin-resistant S. aureus clone from Asia, which is also encountered in Europe. Molecular typing has previously shown that t437 isolates are highly similar regardless of geographical regions or host environments. The present study was aimed at assessing to what extent this high similarity is actually reflected in the production of secreted virulence factors. We therefore profiled the extracellular proteome, representing the main reservoir of virulence factors, of 20 representative clinical isolates by mass spectrometry. The results show that these isolates can be divided into three groups and nine subgroups based on exoproteome abundance signatures. This implies that S. aureus t437 isolates show substantial exoproteome heterogeneity. Nonetheless, 30 highly conserved extracellular proteins, of which about 50% have a predicted role in pathogenesis, were dominantly identified. To approximate the virulence of the 20 investigated isolates, we employed infection models based on Galleria mellonella and HeLa cells. The results show that the grouping of clinical isolates based on their exoproteome profile can be related to virulence. We consider this outcome important as our approach provides a tool to pinpoint differences in virulence among seemingly highly similar clinical isolates of S. aureus. American Chemical Society 2019-05-23 2019-07-05 /pmc/articles/PMC6617432/ /pubmed/31119940 http://dx.doi.org/10.1021/acs.jproteome.9b00179 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Zhao, Xin
Palma Medina, Laura M.
Stobernack, Tim
Glasner, Corinna
de Jong, Anne
Utari, Putri
Setroikromo, Rita
Quax, Wim J.
Otto, Andreas
Becher, Dörte
Buist, Girbe
van Dijl, Jan Maarten
Exoproteome Heterogeneity among Closely Related Staphylococcus aureus t437 Isolates and Possible Implications for Virulence
title Exoproteome Heterogeneity among Closely Related Staphylococcus aureus t437 Isolates and Possible Implications for Virulence
title_full Exoproteome Heterogeneity among Closely Related Staphylococcus aureus t437 Isolates and Possible Implications for Virulence
title_fullStr Exoproteome Heterogeneity among Closely Related Staphylococcus aureus t437 Isolates and Possible Implications for Virulence
title_full_unstemmed Exoproteome Heterogeneity among Closely Related Staphylococcus aureus t437 Isolates and Possible Implications for Virulence
title_short Exoproteome Heterogeneity among Closely Related Staphylococcus aureus t437 Isolates and Possible Implications for Virulence
title_sort exoproteome heterogeneity among closely related staphylococcus aureus t437 isolates and possible implications for virulence
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617432/
https://www.ncbi.nlm.nih.gov/pubmed/31119940
http://dx.doi.org/10.1021/acs.jproteome.9b00179
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