MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus

Kmmig1 as a disrupted mutant of MIG1 encoding a regulator for glucose repression in Kluyveromyces marxianus exhibits a histidine-auxotrophic phenotype. Genome-wide expression analysis revealed that only HIS4 in seven HIS genes for histidine biosynthesis was down-regulated in Kmmig1. Consistently, in...

Descripción completa

Detalles Bibliográficos
Autores principales: Nurcholis, Mochamad, Murata, Masayuki, Limtong, Savitree, Kosaka, Tomoyuki, Yamada, Mamoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617469/
https://www.ncbi.nlm.nih.gov/pubmed/31289320
http://dx.doi.org/10.1038/s41598-019-46411-5
_version_ 1783433700620369920
author Nurcholis, Mochamad
Murata, Masayuki
Limtong, Savitree
Kosaka, Tomoyuki
Yamada, Mamoru
author_facet Nurcholis, Mochamad
Murata, Masayuki
Limtong, Savitree
Kosaka, Tomoyuki
Yamada, Mamoru
author_sort Nurcholis, Mochamad
collection PubMed
description Kmmig1 as a disrupted mutant of MIG1 encoding a regulator for glucose repression in Kluyveromyces marxianus exhibits a histidine-auxotrophic phenotype. Genome-wide expression analysis revealed that only HIS4 in seven HIS genes for histidine biosynthesis was down-regulated in Kmmig1. Consistently, introduction of HIS4 into Kmmig1 suppressed the requirement of histidine. Considering the fact that His4 catalyzes four of ten steps in histidine biosynthesis, K. marxianus has evolved a novel and effective regulation mechanism via Mig1 for the control of histidine biosynthesis. Moreover, RNA-Seq analysis revealed that there were more than 1,000 differentially expressed genes in Kmmig1, suggesting that Mig1 is directly or indirectly involved in the regulation of their expression as a global regulator.
format Online
Article
Text
id pubmed-6617469
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-66174692019-07-18 MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus Nurcholis, Mochamad Murata, Masayuki Limtong, Savitree Kosaka, Tomoyuki Yamada, Mamoru Sci Rep Article Kmmig1 as a disrupted mutant of MIG1 encoding a regulator for glucose repression in Kluyveromyces marxianus exhibits a histidine-auxotrophic phenotype. Genome-wide expression analysis revealed that only HIS4 in seven HIS genes for histidine biosynthesis was down-regulated in Kmmig1. Consistently, introduction of HIS4 into Kmmig1 suppressed the requirement of histidine. Considering the fact that His4 catalyzes four of ten steps in histidine biosynthesis, K. marxianus has evolved a novel and effective regulation mechanism via Mig1 for the control of histidine biosynthesis. Moreover, RNA-Seq analysis revealed that there were more than 1,000 differentially expressed genes in Kmmig1, suggesting that Mig1 is directly or indirectly involved in the regulation of their expression as a global regulator. Nature Publishing Group UK 2019-07-09 /pmc/articles/PMC6617469/ /pubmed/31289320 http://dx.doi.org/10.1038/s41598-019-46411-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nurcholis, Mochamad
Murata, Masayuki
Limtong, Savitree
Kosaka, Tomoyuki
Yamada, Mamoru
MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus
title MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus
title_full MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus
title_fullStr MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus
title_full_unstemmed MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus
title_short MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus
title_sort mig1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast kluyveromyces marxianus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617469/
https://www.ncbi.nlm.nih.gov/pubmed/31289320
http://dx.doi.org/10.1038/s41598-019-46411-5
work_keys_str_mv AT nurcholismochamad mig1asapositiveregulatorforthehistidinebiosynthesispathwayandasaglobalregulatorinthermotolerantyeastkluyveromycesmarxianus
AT muratamasayuki mig1asapositiveregulatorforthehistidinebiosynthesispathwayandasaglobalregulatorinthermotolerantyeastkluyveromycesmarxianus
AT limtongsavitree mig1asapositiveregulatorforthehistidinebiosynthesispathwayandasaglobalregulatorinthermotolerantyeastkluyveromycesmarxianus
AT kosakatomoyuki mig1asapositiveregulatorforthehistidinebiosynthesispathwayandasaglobalregulatorinthermotolerantyeastkluyveromycesmarxianus
AT yamadamamoru mig1asapositiveregulatorforthehistidinebiosynthesispathwayandasaglobalregulatorinthermotolerantyeastkluyveromycesmarxianus

Ejemplares similares