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Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes

BACKGROUND: To illuminate the mechanisms underlying the high-altitude tolerance of Tibetan pig spermatozoa, proteomes of spermatozoa from Tibetan pigs raised in high and low altitudes were compared using a tandem mass tag (TMT)-labeled quantitative proteomics approach. RESULTS: A total of 77 differe...

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Autores principales: Zhao, Yanling, Lu, Xiaoli, Cheng, Zhipeng, Tian, Mengfang, Qiangba Qiang, Yangzong, Fu, Qiang, Ren, Zili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617692/
https://www.ncbi.nlm.nih.gov/pubmed/31291894
http://dx.doi.org/10.1186/s12864-019-5873-0
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author Zhao, Yanling
Lu, Xiaoli
Cheng, Zhipeng
Tian, Mengfang
Qiangba Qiang, Yangzong
Fu, Qiang
Ren, Zili
author_facet Zhao, Yanling
Lu, Xiaoli
Cheng, Zhipeng
Tian, Mengfang
Qiangba Qiang, Yangzong
Fu, Qiang
Ren, Zili
author_sort Zhao, Yanling
collection PubMed
description BACKGROUND: To illuminate the mechanisms underlying the high-altitude tolerance of Tibetan pig spermatozoa, proteomes of spermatozoa from Tibetan pigs raised in high and low altitudes were compared using a tandem mass tag (TMT)-labeled quantitative proteomics approach. RESULTS: A total of 77 differentially expressed proteins (DEPs) were identified. Gene Ontology (GO) analysis revealed DEPs that were predominantly associated with the actin cytoskeleton, the tricarboxylic acid (TCA) cycle, and adenosine triphosphate (ATP) metabolism, and were from 12 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Three subnetworks were significantly enriched and 10 centric proteins were identified by protein-protein interaction (PPI) network analysis. Relative expression levels of the proteins (ATP5H, CYCS, MYH9 and FN1) were confirmed using Western blotting. CONCLUSIONS: Our study is the first to use a tandem mass tag (TMT) approach to analyze Tibetan pig spermatozoa, and provides a foundation to understand the mechanisms underlying the reproductive adaptations of Tibetan pigs to high-altitude environments. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5873-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-66176922019-07-22 Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes Zhao, Yanling Lu, Xiaoli Cheng, Zhipeng Tian, Mengfang Qiangba Qiang, Yangzong Fu, Qiang Ren, Zili BMC Genomics Research Article BACKGROUND: To illuminate the mechanisms underlying the high-altitude tolerance of Tibetan pig spermatozoa, proteomes of spermatozoa from Tibetan pigs raised in high and low altitudes were compared using a tandem mass tag (TMT)-labeled quantitative proteomics approach. RESULTS: A total of 77 differentially expressed proteins (DEPs) were identified. Gene Ontology (GO) analysis revealed DEPs that were predominantly associated with the actin cytoskeleton, the tricarboxylic acid (TCA) cycle, and adenosine triphosphate (ATP) metabolism, and were from 12 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Three subnetworks were significantly enriched and 10 centric proteins were identified by protein-protein interaction (PPI) network analysis. Relative expression levels of the proteins (ATP5H, CYCS, MYH9 and FN1) were confirmed using Western blotting. CONCLUSIONS: Our study is the first to use a tandem mass tag (TMT) approach to analyze Tibetan pig spermatozoa, and provides a foundation to understand the mechanisms underlying the reproductive adaptations of Tibetan pigs to high-altitude environments. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-5873-0) contains supplementary material, which is available to authorized users. BioMed Central 2019-07-10 /pmc/articles/PMC6617692/ /pubmed/31291894 http://dx.doi.org/10.1186/s12864-019-5873-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhao, Yanling
Lu, Xiaoli
Cheng, Zhipeng
Tian, Mengfang
Qiangba Qiang, Yangzong
Fu, Qiang
Ren, Zili
Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes
title Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes
title_full Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes
title_fullStr Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes
title_full_unstemmed Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes
title_short Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes
title_sort comparative proteomic analysis of tibetan pig spermatozoa at high and low altitudes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617692/
https://www.ncbi.nlm.nih.gov/pubmed/31291894
http://dx.doi.org/10.1186/s12864-019-5873-0
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