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RNAi Transfection Results in Lipidome Changes

RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock‐down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two com...

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Autores principales: Özbalci, Cagakan, Storck, Elisabeth M., Eggert, Ulrike S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617754/
https://www.ncbi.nlm.nih.gov/pubmed/31099980
http://dx.doi.org/10.1002/pmic.201800298
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author Özbalci, Cagakan
Storck, Elisabeth M.
Eggert, Ulrike S.
author_facet Özbalci, Cagakan
Storck, Elisabeth M.
Eggert, Ulrike S.
author_sort Özbalci, Cagakan
collection PubMed
description RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock‐down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two common RNA interference (RNAi) transfection reagents, DharmaFECT 1 and INTERFERin, in mock transfections using non‐targeting siRNAs, cause alterations in the lipidome of HeLa cells. Some lipids change in response to both, presumably chemically different, transfection reagents, while other lipid species change only in response to one of the reagents. While the functional implications of these lipidomic alterations remain to be investigated, the authors' experiments suggest that it is important to use appropriate mock transfection controls during RNAi experiments, ideally complemented by an orthogonal perturbation, especially when investigating membrane‐associated phenomena.
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spelling pubmed-66177542019-07-22 RNAi Transfection Results in Lipidome Changes Özbalci, Cagakan Storck, Elisabeth M. Eggert, Ulrike S. Proteomics Dataset Briefs RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock‐down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two common RNA interference (RNAi) transfection reagents, DharmaFECT 1 and INTERFERin, in mock transfections using non‐targeting siRNAs, cause alterations in the lipidome of HeLa cells. Some lipids change in response to both, presumably chemically different, transfection reagents, while other lipid species change only in response to one of the reagents. While the functional implications of these lipidomic alterations remain to be investigated, the authors' experiments suggest that it is important to use appropriate mock transfection controls during RNAi experiments, ideally complemented by an orthogonal perturbation, especially when investigating membrane‐associated phenomena. John Wiley and Sons Inc. 2019-06-13 2019-07 /pmc/articles/PMC6617754/ /pubmed/31099980 http://dx.doi.org/10.1002/pmic.201800298 Text en © 2019 The Authors. Proteomics published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Dataset Briefs
Özbalci, Cagakan
Storck, Elisabeth M.
Eggert, Ulrike S.
RNAi Transfection Results in Lipidome Changes
title RNAi Transfection Results in Lipidome Changes
title_full RNAi Transfection Results in Lipidome Changes
title_fullStr RNAi Transfection Results in Lipidome Changes
title_full_unstemmed RNAi Transfection Results in Lipidome Changes
title_short RNAi Transfection Results in Lipidome Changes
title_sort rnai transfection results in lipidome changes
topic Dataset Briefs
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617754/
https://www.ncbi.nlm.nih.gov/pubmed/31099980
http://dx.doi.org/10.1002/pmic.201800298
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