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Comprehensive analysis of lncRNA–miRNA–mRNA during proliferative phase of rat liver regeneration

This study aims to reveal the regulatory mechanism of lncRNAs–miRNAs–mRNAs network during the proliferative phase of liver regeneration (LR). High‐throughput sequencing technology was performed, and a total of 1,738 differentially expressed lncRNAs (DE lncRNAs), 167 known differentially expressed mi...

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Detalles Bibliográficos
Autores principales: Bai, Haijing, Guo, Jianlin, Chang, Cuifang, Guo, Xueqiang, Xu, Cunshuan, Jin, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617821/
https://www.ncbi.nlm.nih.gov/pubmed/30916358
http://dx.doi.org/10.1002/jcp.28529
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author Bai, Haijing
Guo, Jianlin
Chang, Cuifang
Guo, Xueqiang
Xu, Cunshuan
Jin, Wei
author_facet Bai, Haijing
Guo, Jianlin
Chang, Cuifang
Guo, Xueqiang
Xu, Cunshuan
Jin, Wei
author_sort Bai, Haijing
collection PubMed
description This study aims to reveal the regulatory mechanism of lncRNAs–miRNAs–mRNAs network during the proliferative phase of liver regeneration (LR). High‐throughput sequencing technology was performed, and a total of 1,738 differentially expressed lncRNAs (DE lncRNAs), 167 known differentially expressed miRNAs (DE miRNAs), and 2,727 differentially expressed mRNAs were identified. Then, the target DE lncRNAs and DE mRNAs regulated by the same miRNAs were screened and a ceRNA regulatory network containing 32 miRNAs, 107 lncRNAs, and 270 mRNAs was constructed. Insulin signaling pathway, pyrimidine metabolism, axon guidance, carbohydrate digestion and absorption, and pyruvate metabolism were significantly enriched in the network. Through literature review and the regulatory relationship between lncRNAs and miRNAs, nine core lncRNAs were identified, which might play important roles during the proliferative phase of rat LR. This study analyzed lncRNA–miRNA–mRNA regulatory network for the first time during the proliferative phase of rat LR, providing clues for exploring the mechanism of LR and the treatment of liver diseases.
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spelling pubmed-66178212019-07-22 Comprehensive analysis of lncRNA–miRNA–mRNA during proliferative phase of rat liver regeneration Bai, Haijing Guo, Jianlin Chang, Cuifang Guo, Xueqiang Xu, Cunshuan Jin, Wei J Cell Physiol Original Research Articles This study aims to reveal the regulatory mechanism of lncRNAs–miRNAs–mRNAs network during the proliferative phase of liver regeneration (LR). High‐throughput sequencing technology was performed, and a total of 1,738 differentially expressed lncRNAs (DE lncRNAs), 167 known differentially expressed miRNAs (DE miRNAs), and 2,727 differentially expressed mRNAs were identified. Then, the target DE lncRNAs and DE mRNAs regulated by the same miRNAs were screened and a ceRNA regulatory network containing 32 miRNAs, 107 lncRNAs, and 270 mRNAs was constructed. Insulin signaling pathway, pyrimidine metabolism, axon guidance, carbohydrate digestion and absorption, and pyruvate metabolism were significantly enriched in the network. Through literature review and the regulatory relationship between lncRNAs and miRNAs, nine core lncRNAs were identified, which might play important roles during the proliferative phase of rat LR. This study analyzed lncRNA–miRNA–mRNA regulatory network for the first time during the proliferative phase of rat LR, providing clues for exploring the mechanism of LR and the treatment of liver diseases. John Wiley and Sons Inc. 2019-03-27 2019-10 /pmc/articles/PMC6617821/ /pubmed/30916358 http://dx.doi.org/10.1002/jcp.28529 Text en © 2019 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Articles
Bai, Haijing
Guo, Jianlin
Chang, Cuifang
Guo, Xueqiang
Xu, Cunshuan
Jin, Wei
Comprehensive analysis of lncRNA–miRNA–mRNA during proliferative phase of rat liver regeneration
title Comprehensive analysis of lncRNA–miRNA–mRNA during proliferative phase of rat liver regeneration
title_full Comprehensive analysis of lncRNA–miRNA–mRNA during proliferative phase of rat liver regeneration
title_fullStr Comprehensive analysis of lncRNA–miRNA–mRNA during proliferative phase of rat liver regeneration
title_full_unstemmed Comprehensive analysis of lncRNA–miRNA–mRNA during proliferative phase of rat liver regeneration
title_short Comprehensive analysis of lncRNA–miRNA–mRNA during proliferative phase of rat liver regeneration
title_sort comprehensive analysis of lncrna–mirna–mrna during proliferative phase of rat liver regeneration
topic Original Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617821/
https://www.ncbi.nlm.nih.gov/pubmed/30916358
http://dx.doi.org/10.1002/jcp.28529
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