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High-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats
BACKGROUND: Mupirocin is one of the few antimicrobials active against methicillin-resistant Staphylococcus aureus (MRSA), and is frequently used for the eradication of MRSA nasal colonisation in humans. Initially, mupirocin resistance was recognised in human S. aureus, including MRSA isolates, then...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617863/ https://www.ncbi.nlm.nih.gov/pubmed/31291949 http://dx.doi.org/10.1186/s12917-019-1973-y |
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author | Kizerwetter-Świda, Magdalena Chrobak-Chmiel, Dorota Rzewuska, Magdalena |
author_facet | Kizerwetter-Świda, Magdalena Chrobak-Chmiel, Dorota Rzewuska, Magdalena |
author_sort | Kizerwetter-Świda, Magdalena |
collection | PubMed |
description | BACKGROUND: Mupirocin is one of the few antimicrobials active against methicillin-resistant Staphylococcus aureus (MRSA), and is frequently used for the eradication of MRSA nasal colonisation in humans. Initially, mupirocin resistance was recognised in human S. aureus, including MRSA isolates, then also among coagulase-negative staphylococci (CoNS). Nowadays, mupirocin resistance is occasionally observed in canine staphylococci, along with Staphylococcus pseudintermedius (MRSP) strains, as well as CoNS, which usually show methicillin resistance. In the current study, high-level mupirocin resistance in methicillin-resistant staphylococci isolated from diseased dogs and cats was investigated. RESULTS: Among 140 methicillin-resistant staphylococci isolates from dogs and cats, three showed high-level mupirocin resistance in a screening test using the agar disk diffusion method. One was recognised as methicillin-resistant S. aureus, one as methicillin-resistant S. pseudintermedius, and one as methicillin-resistant Staphylococcus haemolyticus. S. pseudintermedius and S. aureus were isolated from dogs, S. haemolyticus was obtained from a cat. All isolates showed high-level mupirocin resistance, confirmed by minimum inhibitory concentration (MIC) values of above 1024 μg/ml and the presence of the plasmid-located gene ileS2. This is the first report on the detection of high-level mupirocin resistance (HLMR) in S. haemolyticus of feline origin. CONCLUSIONS: This study revealed the occurrence of HLMR in three Staphylococcus isolates obtained from companion animals in Poland. The results of this study indicate that the monitoring of mupirocin resistance in staphylococci of animal origin, especially in methicillin-resistant isolates, is strongly recommended. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-019-1973-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6617863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-66178632019-07-22 High-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats Kizerwetter-Świda, Magdalena Chrobak-Chmiel, Dorota Rzewuska, Magdalena BMC Vet Res Research Article BACKGROUND: Mupirocin is one of the few antimicrobials active against methicillin-resistant Staphylococcus aureus (MRSA), and is frequently used for the eradication of MRSA nasal colonisation in humans. Initially, mupirocin resistance was recognised in human S. aureus, including MRSA isolates, then also among coagulase-negative staphylococci (CoNS). Nowadays, mupirocin resistance is occasionally observed in canine staphylococci, along with Staphylococcus pseudintermedius (MRSP) strains, as well as CoNS, which usually show methicillin resistance. In the current study, high-level mupirocin resistance in methicillin-resistant staphylococci isolated from diseased dogs and cats was investigated. RESULTS: Among 140 methicillin-resistant staphylococci isolates from dogs and cats, three showed high-level mupirocin resistance in a screening test using the agar disk diffusion method. One was recognised as methicillin-resistant S. aureus, one as methicillin-resistant S. pseudintermedius, and one as methicillin-resistant Staphylococcus haemolyticus. S. pseudintermedius and S. aureus were isolated from dogs, S. haemolyticus was obtained from a cat. All isolates showed high-level mupirocin resistance, confirmed by minimum inhibitory concentration (MIC) values of above 1024 μg/ml and the presence of the plasmid-located gene ileS2. This is the first report on the detection of high-level mupirocin resistance (HLMR) in S. haemolyticus of feline origin. CONCLUSIONS: This study revealed the occurrence of HLMR in three Staphylococcus isolates obtained from companion animals in Poland. The results of this study indicate that the monitoring of mupirocin resistance in staphylococci of animal origin, especially in methicillin-resistant isolates, is strongly recommended. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-019-1973-y) contains supplementary material, which is available to authorized users. BioMed Central 2019-07-10 /pmc/articles/PMC6617863/ /pubmed/31291949 http://dx.doi.org/10.1186/s12917-019-1973-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Kizerwetter-Świda, Magdalena Chrobak-Chmiel, Dorota Rzewuska, Magdalena High-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats |
title | High-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats |
title_full | High-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats |
title_fullStr | High-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats |
title_full_unstemmed | High-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats |
title_short | High-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats |
title_sort | high-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617863/ https://www.ncbi.nlm.nih.gov/pubmed/31291949 http://dx.doi.org/10.1186/s12917-019-1973-y |
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