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Using a reaction‐diffusion model to estimate day respiration and reassimilation of (photo)respired CO (2) in leaves
Methods using gas exchange measurements to estimate respiration in the light (day respiration [Formula: see text]) make implicit assumptions about reassimilation of (photo)respired CO (2); however, this reassimilation depends on the positions of mitochondria. We used a reaction‐diffusion model witho...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6618012/ https://www.ncbi.nlm.nih.gov/pubmed/31002400 http://dx.doi.org/10.1111/nph.15857 |
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author | Berghuijs, Herman N. C. Yin, Xinyou Ho, Q. Tri Retta, Moges A. Nicolaï, Bart M. Struik, Paul C. |
author_facet | Berghuijs, Herman N. C. Yin, Xinyou Ho, Q. Tri Retta, Moges A. Nicolaï, Bart M. Struik, Paul C. |
author_sort | Berghuijs, Herman N. C. |
collection | PubMed |
description | Methods using gas exchange measurements to estimate respiration in the light (day respiration [Formula: see text]) make implicit assumptions about reassimilation of (photo)respired CO (2); however, this reassimilation depends on the positions of mitochondria. We used a reaction‐diffusion model without making these assumptions to analyse datasets on gas exchange, chlorophyll fluorescence and anatomy for tomato leaves. We investigated how [Formula: see text] values obtained by the Kok and the Yin methods are affected by these assumptions and how those by the Laisk method are affected by the positions of mitochondria. The Kok method always underestimated [Formula: see text]. Estimates of [Formula: see text] by the Yin method and by the reaction‐diffusion model agreed only for nonphotorespiratory conditions. Both the Yin and Kok methods ignore reassimilation of (photo)respired CO (2), and thus underestimated [Formula: see text] for photorespiratory conditions, but this was less so in the Yin than in the Kok method. Estimates by the Laisk method were affected by assumed positions of mitochondria. It did not work if mitochondria were in the cytosol between the plasmamembrane and the chloroplast envelope. However, mitochondria were found to be most likely between the tonoplast and chloroplasts. Our reaction‐diffusion model effectively estimates [Formula: see text] , enlightens the dependence of [Formula: see text] estimates on reassimilation and clarifies (dis)advantages of existing methods. |
format | Online Article Text |
id | pubmed-6618012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66180122019-07-22 Using a reaction‐diffusion model to estimate day respiration and reassimilation of (photo)respired CO (2) in leaves Berghuijs, Herman N. C. Yin, Xinyou Ho, Q. Tri Retta, Moges A. Nicolaï, Bart M. Struik, Paul C. New Phytol Research Methods using gas exchange measurements to estimate respiration in the light (day respiration [Formula: see text]) make implicit assumptions about reassimilation of (photo)respired CO (2); however, this reassimilation depends on the positions of mitochondria. We used a reaction‐diffusion model without making these assumptions to analyse datasets on gas exchange, chlorophyll fluorescence and anatomy for tomato leaves. We investigated how [Formula: see text] values obtained by the Kok and the Yin methods are affected by these assumptions and how those by the Laisk method are affected by the positions of mitochondria. The Kok method always underestimated [Formula: see text]. Estimates of [Formula: see text] by the Yin method and by the reaction‐diffusion model agreed only for nonphotorespiratory conditions. Both the Yin and Kok methods ignore reassimilation of (photo)respired CO (2), and thus underestimated [Formula: see text] for photorespiratory conditions, but this was less so in the Yin than in the Kok method. Estimates by the Laisk method were affected by assumed positions of mitochondria. It did not work if mitochondria were in the cytosol between the plasmamembrane and the chloroplast envelope. However, mitochondria were found to be most likely between the tonoplast and chloroplasts. Our reaction‐diffusion model effectively estimates [Formula: see text] , enlightens the dependence of [Formula: see text] estimates on reassimilation and clarifies (dis)advantages of existing methods. John Wiley and Sons Inc. 2019-05-11 2019-07 /pmc/articles/PMC6618012/ /pubmed/31002400 http://dx.doi.org/10.1111/nph.15857 Text en © 2019 The Authors. New Phytologist © 2019 New Phytologist Trust This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Berghuijs, Herman N. C. Yin, Xinyou Ho, Q. Tri Retta, Moges A. Nicolaï, Bart M. Struik, Paul C. Using a reaction‐diffusion model to estimate day respiration and reassimilation of (photo)respired CO (2) in leaves |
title | Using a reaction‐diffusion model to estimate day respiration and reassimilation of (photo)respired CO
(2) in leaves |
title_full | Using a reaction‐diffusion model to estimate day respiration and reassimilation of (photo)respired CO
(2) in leaves |
title_fullStr | Using a reaction‐diffusion model to estimate day respiration and reassimilation of (photo)respired CO
(2) in leaves |
title_full_unstemmed | Using a reaction‐diffusion model to estimate day respiration and reassimilation of (photo)respired CO
(2) in leaves |
title_short | Using a reaction‐diffusion model to estimate day respiration and reassimilation of (photo)respired CO
(2) in leaves |
title_sort | using a reaction‐diffusion model to estimate day respiration and reassimilation of (photo)respired co
(2) in leaves |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6618012/ https://www.ncbi.nlm.nih.gov/pubmed/31002400 http://dx.doi.org/10.1111/nph.15857 |
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