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Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations

A set of 185 strains of Candida albicans from patients with vulvovaginal candidiasis (VVC) and from non‐VVC clinical sources in southwest China was analysed. Strains were subjected to genotyping using CAI microsatellite typing and amplification of an intron‐containing region of the 25S rRNA gene. Mi...

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Autores principales: Wang, Meizhu, Cao, Yu, Xia, Maoning, Al‐Hatmi, Abdullah M.S., Ou, Weizheng, Wang, Yanyan, Sibirny, Andriy A., Zhao, Liang, Zou, Chenggang, Liao, Wanqing, Bai, Fengyan, Zhi, Xie, de Hoog, Sybren, Kang, Yingqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6618086/
https://www.ncbi.nlm.nih.gov/pubmed/31037772
http://dx.doi.org/10.1002/yea.3397
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author Wang, Meizhu
Cao, Yu
Xia, Maoning
Al‐Hatmi, Abdullah M.S.
Ou, Weizheng
Wang, Yanyan
Sibirny, Andriy A.
Zhao, Liang
Zou, Chenggang
Liao, Wanqing
Bai, Fengyan
Zhi, Xie
de Hoog, Sybren
Kang, Yingqian
author_facet Wang, Meizhu
Cao, Yu
Xia, Maoning
Al‐Hatmi, Abdullah M.S.
Ou, Weizheng
Wang, Yanyan
Sibirny, Andriy A.
Zhao, Liang
Zou, Chenggang
Liao, Wanqing
Bai, Fengyan
Zhi, Xie
de Hoog, Sybren
Kang, Yingqian
author_sort Wang, Meizhu
collection PubMed
description A set of 185 strains of Candida albicans from patients with vulvovaginal candidiasis (VVC) and from non‐VVC clinical sources in southwest China was analysed. Strains were subjected to genotyping using CAI microsatellite typing and amplification of an intron‐containing region of the 25S rRNA gene. Microsatellite genotypes of strains from non‐VVC sources showed high polymorphism, whereas those of VVC were dominated by few, closely similar genotypes. However, among non‐VVC strains, two genotypes were particularly prevalent in patients with lung cancer. 25S rDNA genotype A was dominant in VVC sources (86.7%), whereas genotypes A, B, and C were rather evenly distributed among non‐VVC sources; known genotypes D and E were not found. In an experimental mouse model, isolates from lung cancer and AIDS patients proved to have higher virulence than VVC strains. Among 156 mice infected with C. albicans, 19 developed non‐invasive urothelial carcinoma. No correlation could be established between parameters of virulence, source of infection, and incidence of carcinoma. C. albicans strains from VVC were less susceptible to itraconazole than the strains from non‐VVC sources, whereas there was small difference in antifungal susceptibility between different 25S rDNA genotypes of C. albicans tested against amphotericin B, itraconazole, fluconazole, and flucytosine.
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spelling pubmed-66180862019-07-22 Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations Wang, Meizhu Cao, Yu Xia, Maoning Al‐Hatmi, Abdullah M.S. Ou, Weizheng Wang, Yanyan Sibirny, Andriy A. Zhao, Liang Zou, Chenggang Liao, Wanqing Bai, Fengyan Zhi, Xie de Hoog, Sybren Kang, Yingqian Yeast Special Issue Articles A set of 185 strains of Candida albicans from patients with vulvovaginal candidiasis (VVC) and from non‐VVC clinical sources in southwest China was analysed. Strains were subjected to genotyping using CAI microsatellite typing and amplification of an intron‐containing region of the 25S rRNA gene. Microsatellite genotypes of strains from non‐VVC sources showed high polymorphism, whereas those of VVC were dominated by few, closely similar genotypes. However, among non‐VVC strains, two genotypes were particularly prevalent in patients with lung cancer. 25S rDNA genotype A was dominant in VVC sources (86.7%), whereas genotypes A, B, and C were rather evenly distributed among non‐VVC sources; known genotypes D and E were not found. In an experimental mouse model, isolates from lung cancer and AIDS patients proved to have higher virulence than VVC strains. Among 156 mice infected with C. albicans, 19 developed non‐invasive urothelial carcinoma. No correlation could be established between parameters of virulence, source of infection, and incidence of carcinoma. C. albicans strains from VVC were less susceptible to itraconazole than the strains from non‐VVC sources, whereas there was small difference in antifungal susceptibility between different 25S rDNA genotypes of C. albicans tested against amphotericin B, itraconazole, fluconazole, and flucytosine. John Wiley and Sons Inc. 2019-06-13 2019-05 /pmc/articles/PMC6618086/ /pubmed/31037772 http://dx.doi.org/10.1002/yea.3397 Text en © 2019 The Authors Yeast Published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Special Issue Articles
Wang, Meizhu
Cao, Yu
Xia, Maoning
Al‐Hatmi, Abdullah M.S.
Ou, Weizheng
Wang, Yanyan
Sibirny, Andriy A.
Zhao, Liang
Zou, Chenggang
Liao, Wanqing
Bai, Fengyan
Zhi, Xie
de Hoog, Sybren
Kang, Yingqian
Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
title Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
title_full Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
title_fullStr Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
title_full_unstemmed Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
title_short Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
title_sort virulence and antifungal susceptibility of microsatellite genotypes of candida albicans from superficial and deep locations
topic Special Issue Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6618086/
https://www.ncbi.nlm.nih.gov/pubmed/31037772
http://dx.doi.org/10.1002/yea.3397
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