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Global Transcriptome Profiling of Multiple Porcine Organs Reveals Toxoplasma gondii-Induced Transcriptional Landscapes
We characterized the porcine tissue transcriptional landscapes that follow Toxoplasma gondii infection. RNAs were isolated from liver, spleen, cerebral cortex, lung, and mesenteric lymph nodes (MLNs) of T. gondii-infected and uninfected (control) pigs at days 6 and 18 postinfection, and were analyze...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6618905/ https://www.ncbi.nlm.nih.gov/pubmed/31333663 http://dx.doi.org/10.3389/fimmu.2019.01531 |
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author | He, Jun-Jun Ma, Jun Wang, Jin-Lei Zhang, Fu-Kai Li, Jie-Xi Zhai, Bin-Tao Wang, Ze-Xiang Elsheikha, Hany M. Zhu, Xing-Quan |
author_facet | He, Jun-Jun Ma, Jun Wang, Jin-Lei Zhang, Fu-Kai Li, Jie-Xi Zhai, Bin-Tao Wang, Ze-Xiang Elsheikha, Hany M. Zhu, Xing-Quan |
author_sort | He, Jun-Jun |
collection | PubMed |
description | We characterized the porcine tissue transcriptional landscapes that follow Toxoplasma gondii infection. RNAs were isolated from liver, spleen, cerebral cortex, lung, and mesenteric lymph nodes (MLNs) of T. gondii-infected and uninfected (control) pigs at days 6 and 18 postinfection, and were analyzed using next-generation sequencing (RNA-seq). T. gondii altered the expression of 178, 476, 199, 201, and 362 transcripts at 6 dpi and 217, 223, 347, 119, and 161 at 18 dpi in the infected brain, liver, lung, MLNs and spleen, respectively. The differentially expressed transcripts (DETs) were grouped into five expression patterns and 10 sub-clusters. Gene Ontology enrichment and pathway analysis revealed that immune-related genes dominated the overall transcriptomic signature and that metabolic processes, such as steroid biosynthesis, and metabolism of lipid and carboxylic acid, were downregulated in infected tissues. Co-expression network analysis identified transcriptional modules associated with host immune response to infection. These findings not only show how T. gondii infection alters porcine transcriptome in a tissue-specific manner, but also offer a gateway for testing new hypotheses regarding human response to T. gondii infection. |
format | Online Article Text |
id | pubmed-6618905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66189052019-07-22 Global Transcriptome Profiling of Multiple Porcine Organs Reveals Toxoplasma gondii-Induced Transcriptional Landscapes He, Jun-Jun Ma, Jun Wang, Jin-Lei Zhang, Fu-Kai Li, Jie-Xi Zhai, Bin-Tao Wang, Ze-Xiang Elsheikha, Hany M. Zhu, Xing-Quan Front Immunol Immunology We characterized the porcine tissue transcriptional landscapes that follow Toxoplasma gondii infection. RNAs were isolated from liver, spleen, cerebral cortex, lung, and mesenteric lymph nodes (MLNs) of T. gondii-infected and uninfected (control) pigs at days 6 and 18 postinfection, and were analyzed using next-generation sequencing (RNA-seq). T. gondii altered the expression of 178, 476, 199, 201, and 362 transcripts at 6 dpi and 217, 223, 347, 119, and 161 at 18 dpi in the infected brain, liver, lung, MLNs and spleen, respectively. The differentially expressed transcripts (DETs) were grouped into five expression patterns and 10 sub-clusters. Gene Ontology enrichment and pathway analysis revealed that immune-related genes dominated the overall transcriptomic signature and that metabolic processes, such as steroid biosynthesis, and metabolism of lipid and carboxylic acid, were downregulated in infected tissues. Co-expression network analysis identified transcriptional modules associated with host immune response to infection. These findings not only show how T. gondii infection alters porcine transcriptome in a tissue-specific manner, but also offer a gateway for testing new hypotheses regarding human response to T. gondii infection. Frontiers Media S.A. 2019-07-03 /pmc/articles/PMC6618905/ /pubmed/31333663 http://dx.doi.org/10.3389/fimmu.2019.01531 Text en Copyright © 2019 He, Ma, Wang, Zhang, Li, Zhai, Wang, Elsheikha and Zhu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology He, Jun-Jun Ma, Jun Wang, Jin-Lei Zhang, Fu-Kai Li, Jie-Xi Zhai, Bin-Tao Wang, Ze-Xiang Elsheikha, Hany M. Zhu, Xing-Quan Global Transcriptome Profiling of Multiple Porcine Organs Reveals Toxoplasma gondii-Induced Transcriptional Landscapes |
title | Global Transcriptome Profiling of Multiple Porcine Organs Reveals Toxoplasma gondii-Induced Transcriptional Landscapes |
title_full | Global Transcriptome Profiling of Multiple Porcine Organs Reveals Toxoplasma gondii-Induced Transcriptional Landscapes |
title_fullStr | Global Transcriptome Profiling of Multiple Porcine Organs Reveals Toxoplasma gondii-Induced Transcriptional Landscapes |
title_full_unstemmed | Global Transcriptome Profiling of Multiple Porcine Organs Reveals Toxoplasma gondii-Induced Transcriptional Landscapes |
title_short | Global Transcriptome Profiling of Multiple Porcine Organs Reveals Toxoplasma gondii-Induced Transcriptional Landscapes |
title_sort | global transcriptome profiling of multiple porcine organs reveals toxoplasma gondii-induced transcriptional landscapes |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6618905/ https://www.ncbi.nlm.nih.gov/pubmed/31333663 http://dx.doi.org/10.3389/fimmu.2019.01531 |
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