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Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls

Emerging evidence suggests that altered intestinal microbiota plays an important role in the pathogenesis of many liver diseases, mainly by promoting inflammation via the “intestinal microbiota-immunity-liver” axis. We aimed to investigate the fecal microbiome of liver recipients with abnormal/norma...

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Autores principales: Lu, Hai-Feng, Ren, Zhi-Gang, Li, Ang, Zhang, Hua, Xu, Shao-Yan, Jiang, Jian-Wen, Zhou, Lin, Ling, Qi, Wang, Bao-Hong, Cui, Guang-Ying, Chen, Xin-Hua, Zheng, Shu-Sen, Li, Lan-Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619441/
https://www.ncbi.nlm.nih.gov/pubmed/31333622
http://dx.doi.org/10.3389/fmicb.2019.01518
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author Lu, Hai-Feng
Ren, Zhi-Gang
Li, Ang
Zhang, Hua
Xu, Shao-Yan
Jiang, Jian-Wen
Zhou, Lin
Ling, Qi
Wang, Bao-Hong
Cui, Guang-Ying
Chen, Xin-Hua
Zheng, Shu-Sen
Li, Lan-Juan
author_facet Lu, Hai-Feng
Ren, Zhi-Gang
Li, Ang
Zhang, Hua
Xu, Shao-Yan
Jiang, Jian-Wen
Zhou, Lin
Ling, Qi
Wang, Bao-Hong
Cui, Guang-Ying
Chen, Xin-Hua
Zheng, Shu-Sen
Li, Lan-Juan
author_sort Lu, Hai-Feng
collection PubMed
description Emerging evidence suggests that altered intestinal microbiota plays an important role in the pathogenesis of many liver diseases, mainly by promoting inflammation via the “intestinal microbiota-immunity-liver” axis. We aimed to investigate the fecal microbiome of liver recipients with abnormal/normal liver function using 16S rRNA gene sequencing. Fecal samples were collected from 90 liver recipients [42 with abnormal liver function (Group LT_A) and 48 with normal liver function (Group LT_N)] and 61 age- and gender-matched healthy controls (HCs). Fecal microbiomes were analyzed for comparative composition, diversity, and richness of microbial communities. Principal coordinates analysis successfully distinguished the fecal microbiomes of recipients in Group LT_A from healthy subjects, with the significant decrease of fecal microbiome diversity in recipients in Group LT_A. Other than a higher relative abundance of opportunistic pathogens such as Klebsiella and Escherichia/Shigella in all liver recipients, the main difference in gut microbiome composition between liver recipients and HC was the lower relative abundance of beneficial butyrate-producing bacteria in the recipients. Importantly, we established a fecal microbiome index (specific alterations in Staphylococcus and Prevotella) that could be used to distinguish Group LT_A from Group LT_N, with an area under the receiver operating characteristic curve value of 0.801 and sensitivity and specificity values of 0.771 and 0.786, respectively. These findings revealed unique gut microbial characteristics of liver recipients with abnormal and normal liver functions, and identified fecal microbial risk indicators of abnormal liver function in liver recipients.
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spelling pubmed-66194412019-07-22 Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls Lu, Hai-Feng Ren, Zhi-Gang Li, Ang Zhang, Hua Xu, Shao-Yan Jiang, Jian-Wen Zhou, Lin Ling, Qi Wang, Bao-Hong Cui, Guang-Ying Chen, Xin-Hua Zheng, Shu-Sen Li, Lan-Juan Front Microbiol Microbiology Emerging evidence suggests that altered intestinal microbiota plays an important role in the pathogenesis of many liver diseases, mainly by promoting inflammation via the “intestinal microbiota-immunity-liver” axis. We aimed to investigate the fecal microbiome of liver recipients with abnormal/normal liver function using 16S rRNA gene sequencing. Fecal samples were collected from 90 liver recipients [42 with abnormal liver function (Group LT_A) and 48 with normal liver function (Group LT_N)] and 61 age- and gender-matched healthy controls (HCs). Fecal microbiomes were analyzed for comparative composition, diversity, and richness of microbial communities. Principal coordinates analysis successfully distinguished the fecal microbiomes of recipients in Group LT_A from healthy subjects, with the significant decrease of fecal microbiome diversity in recipients in Group LT_A. Other than a higher relative abundance of opportunistic pathogens such as Klebsiella and Escherichia/Shigella in all liver recipients, the main difference in gut microbiome composition between liver recipients and HC was the lower relative abundance of beneficial butyrate-producing bacteria in the recipients. Importantly, we established a fecal microbiome index (specific alterations in Staphylococcus and Prevotella) that could be used to distinguish Group LT_A from Group LT_N, with an area under the receiver operating characteristic curve value of 0.801 and sensitivity and specificity values of 0.771 and 0.786, respectively. These findings revealed unique gut microbial characteristics of liver recipients with abnormal and normal liver functions, and identified fecal microbial risk indicators of abnormal liver function in liver recipients. Frontiers Media S.A. 2019-07-03 /pmc/articles/PMC6619441/ /pubmed/31333622 http://dx.doi.org/10.3389/fmicb.2019.01518 Text en Copyright © 2019 Lu, Ren, Li, Zhang, Xu, Jiang, Zhou, Ling, Wang, Cui, Chen, Zheng and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lu, Hai-Feng
Ren, Zhi-Gang
Li, Ang
Zhang, Hua
Xu, Shao-Yan
Jiang, Jian-Wen
Zhou, Lin
Ling, Qi
Wang, Bao-Hong
Cui, Guang-Ying
Chen, Xin-Hua
Zheng, Shu-Sen
Li, Lan-Juan
Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls
title Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls
title_full Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls
title_fullStr Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls
title_full_unstemmed Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls
title_short Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls
title_sort fecal microbiome data distinguish liver recipients with normal and abnormal liver function from healthy controls
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619441/
https://www.ncbi.nlm.nih.gov/pubmed/31333622
http://dx.doi.org/10.3389/fmicb.2019.01518
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