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P53 expression in various types of hydropic placentas (through ploidy analysis as a complementary tool in diagnosis of samples)

BACKGROUND: Placentas characterized by hydropic swelling of chorionic villi occur in a spectrum of pathological conditions including hydropic abortion (HA), partial hydatidiform mole (PHM) and complete hydatidiform mole (CHM). The purpose of this study was to investigate whether the expression of p5...

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Autores principales: Khooei, Alireza, Atabaki Pasdar, Fatemeh, Fazel, Alireza, Mahmoudi, Mahmoud, Nikravesh, Mohammad Reza, Daneshmand Shahbazian, Shahrzad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Babol University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619464/
https://www.ncbi.nlm.nih.gov/pubmed/31363399
http://dx.doi.org/10.22088/cjim.10.2.205
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author Khooei, Alireza
Atabaki Pasdar, Fatemeh
Fazel, Alireza
Mahmoudi, Mahmoud
Nikravesh, Mohammad Reza
Daneshmand Shahbazian, Shahrzad
author_facet Khooei, Alireza
Atabaki Pasdar, Fatemeh
Fazel, Alireza
Mahmoudi, Mahmoud
Nikravesh, Mohammad Reza
Daneshmand Shahbazian, Shahrzad
author_sort Khooei, Alireza
collection PubMed
description BACKGROUND: Placentas characterized by hydropic swelling of chorionic villi occur in a spectrum of pathological conditions including hydropic abortion (HA), partial hydatidiform mole (PHM) and complete hydatidiform mole (CHM). The purpose of this study was to investigate whether the expression of p53 tumour suppressor protein could differentiate these various types of hydropic placentas. METHODS: p53 immunohistochemical staining was performed in 19 molar (8 PHM and 11 CHM) and 10 non-molar (HA) formalin-fixed, paraffin-embedded tissue samples. Ploidy analysis using flow cytometry was performed as a complementary tool in diagnosis of samples. RESULTS: DNA histograms obtained from all samples had confirmed diploidy in HAs and CHMs and triploidy in PHMs. p53 immunoreactivity was assessed in villous cytotrophoblasts, syncytiotrophoblasts and stromal cells. The p53 positive reaction was predominantly observed in the nuclei of cytotrophoblastic cells and rarely in stromal cells, no reaction was seen in syncytiotrophoblasts. The mean percentage of p53 positive cells were 6.10±3.75 for HA, 25.87±13.4 for PHM and 39.83±18.76 for CHM. There was a significant difference in P53 immunoreactivity of cytotrophoblastic cells between CHM and HA (P<0.001), and between PHM and HA (P=0.004). There was no significant difference in immunohistochemical reactivity between CHM and PHM (P=0.068). CONCLUSION: This study confirms that p53 immunostaining may be helpful in distinguishing complete and partial hydatidiform mole from hydropic abortion, but not complete hydatidiform mole from partial hydatidiform mole.
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spelling pubmed-66194642019-07-30 P53 expression in various types of hydropic placentas (through ploidy analysis as a complementary tool in diagnosis of samples) Khooei, Alireza Atabaki Pasdar, Fatemeh Fazel, Alireza Mahmoudi, Mahmoud Nikravesh, Mohammad Reza Daneshmand Shahbazian, Shahrzad Caspian J Intern Med Original Article BACKGROUND: Placentas characterized by hydropic swelling of chorionic villi occur in a spectrum of pathological conditions including hydropic abortion (HA), partial hydatidiform mole (PHM) and complete hydatidiform mole (CHM). The purpose of this study was to investigate whether the expression of p53 tumour suppressor protein could differentiate these various types of hydropic placentas. METHODS: p53 immunohistochemical staining was performed in 19 molar (8 PHM and 11 CHM) and 10 non-molar (HA) formalin-fixed, paraffin-embedded tissue samples. Ploidy analysis using flow cytometry was performed as a complementary tool in diagnosis of samples. RESULTS: DNA histograms obtained from all samples had confirmed diploidy in HAs and CHMs and triploidy in PHMs. p53 immunoreactivity was assessed in villous cytotrophoblasts, syncytiotrophoblasts and stromal cells. The p53 positive reaction was predominantly observed in the nuclei of cytotrophoblastic cells and rarely in stromal cells, no reaction was seen in syncytiotrophoblasts. The mean percentage of p53 positive cells were 6.10±3.75 for HA, 25.87±13.4 for PHM and 39.83±18.76 for CHM. There was a significant difference in P53 immunoreactivity of cytotrophoblastic cells between CHM and HA (P<0.001), and between PHM and HA (P=0.004). There was no significant difference in immunohistochemical reactivity between CHM and PHM (P=0.068). CONCLUSION: This study confirms that p53 immunostaining may be helpful in distinguishing complete and partial hydatidiform mole from hydropic abortion, but not complete hydatidiform mole from partial hydatidiform mole. Babol University of Medical Sciences 2019 /pmc/articles/PMC6619464/ /pubmed/31363399 http://dx.doi.org/10.22088/cjim.10.2.205 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Khooei, Alireza
Atabaki Pasdar, Fatemeh
Fazel, Alireza
Mahmoudi, Mahmoud
Nikravesh, Mohammad Reza
Daneshmand Shahbazian, Shahrzad
P53 expression in various types of hydropic placentas (through ploidy analysis as a complementary tool in diagnosis of samples)
title P53 expression in various types of hydropic placentas (through ploidy analysis as a complementary tool in diagnosis of samples)
title_full P53 expression in various types of hydropic placentas (through ploidy analysis as a complementary tool in diagnosis of samples)
title_fullStr P53 expression in various types of hydropic placentas (through ploidy analysis as a complementary tool in diagnosis of samples)
title_full_unstemmed P53 expression in various types of hydropic placentas (through ploidy analysis as a complementary tool in diagnosis of samples)
title_short P53 expression in various types of hydropic placentas (through ploidy analysis as a complementary tool in diagnosis of samples)
title_sort p53 expression in various types of hydropic placentas (through ploidy analysis as a complementary tool in diagnosis of samples)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619464/
https://www.ncbi.nlm.nih.gov/pubmed/31363399
http://dx.doi.org/10.22088/cjim.10.2.205
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