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Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system

Nanomedicine is an emerging area in the medical field, particularly in the treatment of cancers. Nanostructured lipid carrier (NLC) was shown to be a good nanoparticulated carrier for the delivery of tamoxifen (TAM). In this study, the tamoxifen-loaded erythropoietin-coated nanostructured lipid carr...

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Autores principales: Beh, Chaw Yee, Rasedee, Abdullah, Selvarajah, Gayathri Thevi, Yazan, Latifah Saiful, Omar, Abdul Rahman, Foong, Jia Ning, How, Chee Wun, Foo, Jhi Biau
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619690/
https://www.ncbi.nlm.nih.gov/pubmed/31291309
http://dx.doi.org/10.1371/journal.pone.0219285
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author Beh, Chaw Yee
Rasedee, Abdullah
Selvarajah, Gayathri Thevi
Yazan, Latifah Saiful
Omar, Abdul Rahman
Foong, Jia Ning
How, Chee Wun
Foo, Jhi Biau
author_facet Beh, Chaw Yee
Rasedee, Abdullah
Selvarajah, Gayathri Thevi
Yazan, Latifah Saiful
Omar, Abdul Rahman
Foong, Jia Ning
How, Chee Wun
Foo, Jhi Biau
author_sort Beh, Chaw Yee
collection PubMed
description Nanomedicine is an emerging area in the medical field, particularly in the treatment of cancers. Nanostructured lipid carrier (NLC) was shown to be a good nanoparticulated carrier for the delivery of tamoxifen (TAM). In this study, the tamoxifen-loaded erythropoietin-coated nanostructured lipid carriers (EPO-TAMNLC) were developed to enhance the anti-cancer properties and targetability of TAM, using EPO as the homing ligand for EPO receptors (EpoRs) on breast cancer tissue cells. Tamoxifen-loaded NLC (TAMNLC) was used for comparison. The LA7 cells and LA7 cell-induced rat mammary gland tumor were used as models in the study. Immunocytochemistry staining showed that LA7 cells express estrogen receptors (ERs) and EpoRs. EPO-TAMNLC and TAMNLC significantly (p<0.05) inhibited proliferation of LA7 in dose- and time-dependent manner. EPO-TAMNLC induced apoptosis and G(0)/G(1) cell cycle arrest of LA7 cells. Both drug delivery systems showed anti-mammary gland tumor properties. At an intravenous dose of 5 mg kg(-1) body weight, EPO-TAMNLC and TAMNLC were not toxic to rats, suggesting that both are safe therapeutic compounds. In conclusion, EPO-TAMNLC is not only a unique drug delivery system because of the dual drug-loading feature, but also potentially highly specific in the targeting of breast cancer tissues positive for ERs and EpoRs. The incorporation of TAM into NLC with and without EPO coat had significantly (p<0.05) improved specificity and safety of the drug carriers in the treatment of mammary gland tumors.
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spelling pubmed-66196902019-07-25 Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system Beh, Chaw Yee Rasedee, Abdullah Selvarajah, Gayathri Thevi Yazan, Latifah Saiful Omar, Abdul Rahman Foong, Jia Ning How, Chee Wun Foo, Jhi Biau PLoS One Research Article Nanomedicine is an emerging area in the medical field, particularly in the treatment of cancers. Nanostructured lipid carrier (NLC) was shown to be a good nanoparticulated carrier for the delivery of tamoxifen (TAM). In this study, the tamoxifen-loaded erythropoietin-coated nanostructured lipid carriers (EPO-TAMNLC) were developed to enhance the anti-cancer properties and targetability of TAM, using EPO as the homing ligand for EPO receptors (EpoRs) on breast cancer tissue cells. Tamoxifen-loaded NLC (TAMNLC) was used for comparison. The LA7 cells and LA7 cell-induced rat mammary gland tumor were used as models in the study. Immunocytochemistry staining showed that LA7 cells express estrogen receptors (ERs) and EpoRs. EPO-TAMNLC and TAMNLC significantly (p<0.05) inhibited proliferation of LA7 in dose- and time-dependent manner. EPO-TAMNLC induced apoptosis and G(0)/G(1) cell cycle arrest of LA7 cells. Both drug delivery systems showed anti-mammary gland tumor properties. At an intravenous dose of 5 mg kg(-1) body weight, EPO-TAMNLC and TAMNLC were not toxic to rats, suggesting that both are safe therapeutic compounds. In conclusion, EPO-TAMNLC is not only a unique drug delivery system because of the dual drug-loading feature, but also potentially highly specific in the targeting of breast cancer tissues positive for ERs and EpoRs. The incorporation of TAM into NLC with and without EPO coat had significantly (p<0.05) improved specificity and safety of the drug carriers in the treatment of mammary gland tumors. Public Library of Science 2019-07-10 /pmc/articles/PMC6619690/ /pubmed/31291309 http://dx.doi.org/10.1371/journal.pone.0219285 Text en © 2019 Beh et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Beh, Chaw Yee
Rasedee, Abdullah
Selvarajah, Gayathri Thevi
Yazan, Latifah Saiful
Omar, Abdul Rahman
Foong, Jia Ning
How, Chee Wun
Foo, Jhi Biau
Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_full Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_fullStr Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_full_unstemmed Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_short Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_sort enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619690/
https://www.ncbi.nlm.nih.gov/pubmed/31291309
http://dx.doi.org/10.1371/journal.pone.0219285
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