Relationship of transcriptional markers to Leydig cell number in the mouse testis

OBJECTIVES: The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically est...

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Detalles Bibliográficos
Autores principales: Rebourcet, Diane, Monteiro, Ana, Cruickshanks, Lyndsey, Jeffery, Nathan, Smith, Sarah, Milne, Laura, O’Shaughnessy, Peter J., Smith, Lee B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619764/
https://www.ncbi.nlm.nih.gov/pubmed/31291327
http://dx.doi.org/10.1371/journal.pone.0219524
Descripción
Sumario:OBJECTIVES: The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically established the size of the Leydig cell population, to try to identify transcriptional biomarkers indicative of population size. RESULTS: Following characterisation of the Leydig cell population after Sertoli cell ablation in neonatal life or adulthood, we identified Hsd3b1 transcript levels as a potential indicator of Leydig cell number with utility for informing decision-making on whether to engage in time-consuming stereological cell counting analysis.

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