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The proteome of extracellular vesicles released by clastic cells differs based on their substrate

Extracellular vesicles (EVs) from osteoclasts are important regulators in intercellular communication. Here, we investigated the proteome of EVs from clastic cells plated on plastic (clasts), bone (osteoclasts) and dentin (odontoclasts) by two-dimensional high performance liquid chromatography mass...

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Autores principales: Rody, Wellington J., Chamberlain, Casey A., Emory-Carter, Alyssa K., McHugh, Kevin P., Wallet, Shannon M., Spicer, Victor, Krokhin, Oleg, Holliday, L. Shannon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619814/
https://www.ncbi.nlm.nih.gov/pubmed/31291376
http://dx.doi.org/10.1371/journal.pone.0219602
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author Rody, Wellington J.
Chamberlain, Casey A.
Emory-Carter, Alyssa K.
McHugh, Kevin P.
Wallet, Shannon M.
Spicer, Victor
Krokhin, Oleg
Holliday, L. Shannon
author_facet Rody, Wellington J.
Chamberlain, Casey A.
Emory-Carter, Alyssa K.
McHugh, Kevin P.
Wallet, Shannon M.
Spicer, Victor
Krokhin, Oleg
Holliday, L. Shannon
author_sort Rody, Wellington J.
collection PubMed
description Extracellular vesicles (EVs) from osteoclasts are important regulators in intercellular communication. Here, we investigated the proteome of EVs from clastic cells plated on plastic (clasts), bone (osteoclasts) and dentin (odontoclasts) by two-dimensional high performance liquid chromatography mass spectrometry seeking differences attributable to distinct mineralized matrices. A total of 1,952 proteins were identified. Of the 500 most abundant proteins in EVs, osteoclast and odontoclast EVs were 83.3% identical, while clasts shared 70.7% of the proteins with osteoclasts and 74.2% of proteins with odontoclasts. For each protein, the differences between the total ion count values were mapped to an expression ratio histogram (Z-score) in order to detect proteins differentially expressed. Stabilin-1 and macrophage mannose receptor-1 were significantly-enriched in EVs from odontoclasts compared with osteoclasts (Z = 2.45, Z = 3.34) and clasts (Z = 13.86, Z = 1.81) and were abundant in odontoclast EVs. Numerous less abundant proteins were differentially-enriched. Subunits of known protein complexes were abundant in clastic EVs, and were present at levels consistent with them being in assembled protein complexes. These included the proteasome, COP1, COP9, the T complex and a novel sub-complex of vacuolar H(+)-ATPase (V-ATPase), which included the (pro) renin receptor. The (pro) renin receptor was immunoprecipitated using an anti-E-subunit antibody from detergent-solubilized EVs, supporting the idea that the V-ATPase subunits present were in the same protein complex. We conclude that the protein composition of EVs released by clastic cells changes based on the substrate. Clastic EVs are enriched in various protein complexes including a previously undescribed V-ATPase sub-complex.
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spelling pubmed-66198142019-07-25 The proteome of extracellular vesicles released by clastic cells differs based on their substrate Rody, Wellington J. Chamberlain, Casey A. Emory-Carter, Alyssa K. McHugh, Kevin P. Wallet, Shannon M. Spicer, Victor Krokhin, Oleg Holliday, L. Shannon PLoS One Research Article Extracellular vesicles (EVs) from osteoclasts are important regulators in intercellular communication. Here, we investigated the proteome of EVs from clastic cells plated on plastic (clasts), bone (osteoclasts) and dentin (odontoclasts) by two-dimensional high performance liquid chromatography mass spectrometry seeking differences attributable to distinct mineralized matrices. A total of 1,952 proteins were identified. Of the 500 most abundant proteins in EVs, osteoclast and odontoclast EVs were 83.3% identical, while clasts shared 70.7% of the proteins with osteoclasts and 74.2% of proteins with odontoclasts. For each protein, the differences between the total ion count values were mapped to an expression ratio histogram (Z-score) in order to detect proteins differentially expressed. Stabilin-1 and macrophage mannose receptor-1 were significantly-enriched in EVs from odontoclasts compared with osteoclasts (Z = 2.45, Z = 3.34) and clasts (Z = 13.86, Z = 1.81) and were abundant in odontoclast EVs. Numerous less abundant proteins were differentially-enriched. Subunits of known protein complexes were abundant in clastic EVs, and were present at levels consistent with them being in assembled protein complexes. These included the proteasome, COP1, COP9, the T complex and a novel sub-complex of vacuolar H(+)-ATPase (V-ATPase), which included the (pro) renin receptor. The (pro) renin receptor was immunoprecipitated using an anti-E-subunit antibody from detergent-solubilized EVs, supporting the idea that the V-ATPase subunits present were in the same protein complex. We conclude that the protein composition of EVs released by clastic cells changes based on the substrate. Clastic EVs are enriched in various protein complexes including a previously undescribed V-ATPase sub-complex. Public Library of Science 2019-07-10 /pmc/articles/PMC6619814/ /pubmed/31291376 http://dx.doi.org/10.1371/journal.pone.0219602 Text en © 2019 Rody Jr. et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rody, Wellington J.
Chamberlain, Casey A.
Emory-Carter, Alyssa K.
McHugh, Kevin P.
Wallet, Shannon M.
Spicer, Victor
Krokhin, Oleg
Holliday, L. Shannon
The proteome of extracellular vesicles released by clastic cells differs based on their substrate
title The proteome of extracellular vesicles released by clastic cells differs based on their substrate
title_full The proteome of extracellular vesicles released by clastic cells differs based on their substrate
title_fullStr The proteome of extracellular vesicles released by clastic cells differs based on their substrate
title_full_unstemmed The proteome of extracellular vesicles released by clastic cells differs based on their substrate
title_short The proteome of extracellular vesicles released by clastic cells differs based on their substrate
title_sort proteome of extracellular vesicles released by clastic cells differs based on their substrate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619814/
https://www.ncbi.nlm.nih.gov/pubmed/31291376
http://dx.doi.org/10.1371/journal.pone.0219602
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