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Studies of a rice sterile mutant sstl from the TRIM collection

BACKGROUND: Rice (Oryza sativa) is one of the main crops in the world, and more than 3.9 billion people will consume rice by 2025. Sterility significantly affects rice production and leads to yield defects. The undeveloped anthers or abnormal pollen represent serious defects in rice male sterility....

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Autores principales: Chang, Chia-Ling, Serapion, Jerry C., Hung, Han-Hui, Lin, Yan-Cheng, Tsai, Yuan-Ching, Jane, Wann-Neng, Chang, Men-Chi, Lai, Ming-Hsin, Hsing, Yue-ie C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620220/
https://www.ncbi.nlm.nih.gov/pubmed/31292815
http://dx.doi.org/10.1186/s40529-019-0260-3
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author Chang, Chia-Ling
Serapion, Jerry C.
Hung, Han-Hui
Lin, Yan-Cheng
Tsai, Yuan-Ching
Jane, Wann-Neng
Chang, Men-Chi
Lai, Ming-Hsin
Hsing, Yue-ie C.
author_facet Chang, Chia-Ling
Serapion, Jerry C.
Hung, Han-Hui
Lin, Yan-Cheng
Tsai, Yuan-Ching
Jane, Wann-Neng
Chang, Men-Chi
Lai, Ming-Hsin
Hsing, Yue-ie C.
author_sort Chang, Chia-Ling
collection PubMed
description BACKGROUND: Rice (Oryza sativa) is one of the main crops in the world, and more than 3.9 billion people will consume rice by 2025. Sterility significantly affects rice production and leads to yield defects. The undeveloped anthers or abnormal pollen represent serious defects in rice male sterility. Therefore, understanding the mechanism of male sterility is an important task. Here, we investigated a rice sterile mutant according to its developmental morphology and transcriptional profiles. RESULTS: An untagged T-DNA insertional mutant showed defective pollen and abnormal anthers as compared with its semi-sterile mutant (sstl) progeny segregates. Transcriptomic analysis of sterile sstl-s revealed several biosynthesis pathways, such as downregulated cell wall, lipids, secondary metabolism, and starch synthesis. This downregulation is consistent with the morphological characterization of sstl-s anthers with irregular exine, absence of intine, no starch accumulation in pollen grains and no accumulated flavonoids in anthers. Moreover, defective microsporangia development led to abnormal anther locule and aborted microspores. The downregulated lipids, starch, and cell wall synthesis-related genes resulted in loss of fertility. CONCLUSIONS: We illustrate the importance of microsporangia in the development of anthers and functional microspores. Abnormal development of pollen grains, pollen wall, anther locule, etc. result in severe yield reduction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40529-019-0260-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-66202202019-07-28 Studies of a rice sterile mutant sstl from the TRIM collection Chang, Chia-Ling Serapion, Jerry C. Hung, Han-Hui Lin, Yan-Cheng Tsai, Yuan-Ching Jane, Wann-Neng Chang, Men-Chi Lai, Ming-Hsin Hsing, Yue-ie C. Bot Stud Original Article BACKGROUND: Rice (Oryza sativa) is one of the main crops in the world, and more than 3.9 billion people will consume rice by 2025. Sterility significantly affects rice production and leads to yield defects. The undeveloped anthers or abnormal pollen represent serious defects in rice male sterility. Therefore, understanding the mechanism of male sterility is an important task. Here, we investigated a rice sterile mutant according to its developmental morphology and transcriptional profiles. RESULTS: An untagged T-DNA insertional mutant showed defective pollen and abnormal anthers as compared with its semi-sterile mutant (sstl) progeny segregates. Transcriptomic analysis of sterile sstl-s revealed several biosynthesis pathways, such as downregulated cell wall, lipids, secondary metabolism, and starch synthesis. This downregulation is consistent with the morphological characterization of sstl-s anthers with irregular exine, absence of intine, no starch accumulation in pollen grains and no accumulated flavonoids in anthers. Moreover, defective microsporangia development led to abnormal anther locule and aborted microspores. The downregulated lipids, starch, and cell wall synthesis-related genes resulted in loss of fertility. CONCLUSIONS: We illustrate the importance of microsporangia in the development of anthers and functional microspores. Abnormal development of pollen grains, pollen wall, anther locule, etc. result in severe yield reduction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40529-019-0260-3) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-07-10 /pmc/articles/PMC6620220/ /pubmed/31292815 http://dx.doi.org/10.1186/s40529-019-0260-3 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Chang, Chia-Ling
Serapion, Jerry C.
Hung, Han-Hui
Lin, Yan-Cheng
Tsai, Yuan-Ching
Jane, Wann-Neng
Chang, Men-Chi
Lai, Ming-Hsin
Hsing, Yue-ie C.
Studies of a rice sterile mutant sstl from the TRIM collection
title Studies of a rice sterile mutant sstl from the TRIM collection
title_full Studies of a rice sterile mutant sstl from the TRIM collection
title_fullStr Studies of a rice sterile mutant sstl from the TRIM collection
title_full_unstemmed Studies of a rice sterile mutant sstl from the TRIM collection
title_short Studies of a rice sterile mutant sstl from the TRIM collection
title_sort studies of a rice sterile mutant sstl from the trim collection
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620220/
https://www.ncbi.nlm.nih.gov/pubmed/31292815
http://dx.doi.org/10.1186/s40529-019-0260-3
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