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Thermophysical Fingerprinting of Probiotic-Based Products

Variability in efficacy and safety is a worldwide concern with commercial probiotics for their growing and inevitable use in food and health sectors. Here, we introduce a probiotic thermophysical fingerprinting methodology using a coupling thermogravimetry and differential scanning calorimetry. Qual...

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Detalles Bibliográficos
Autores principales: Razafindralambo, Hary, Razafindralambo, Aurélie, Blecker, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620332/
https://www.ncbi.nlm.nih.gov/pubmed/31292519
http://dx.doi.org/10.1038/s41598-019-46469-1
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author Razafindralambo, Hary
Razafindralambo, Aurélie
Blecker, Christophe
author_facet Razafindralambo, Hary
Razafindralambo, Aurélie
Blecker, Christophe
author_sort Razafindralambo, Hary
collection PubMed
description Variability in efficacy and safety is a worldwide concern with commercial probiotics for their growing and inevitable use in food and health sectors. Here, we introduce a probiotic thermophysical fingerprinting methodology using a coupling thermogravimetry and differential scanning calorimetry. Qualitative and quantitative information on the material decomposition and transition phases is provided under heating conditions. By monitoring the changes in both mass and internal energy over temperature and time, a couple of thermal data at the maximum decomposition steps allow the creation of a unique and global product identity, depending on both strain and excipient components. We demonstrate that each powder formulation of monostrain and multistrain from different lots and origins have a unique thermophysical profile. Our approach also provides information on the formulation thermostability and additive/excipient composition. An original fingerprint form is proposed by converting the generated thermal data sequence into a star-like pattern for a perspective library construction.
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spelling pubmed-66203322019-07-18 Thermophysical Fingerprinting of Probiotic-Based Products Razafindralambo, Hary Razafindralambo, Aurélie Blecker, Christophe Sci Rep Article Variability in efficacy and safety is a worldwide concern with commercial probiotics for their growing and inevitable use in food and health sectors. Here, we introduce a probiotic thermophysical fingerprinting methodology using a coupling thermogravimetry and differential scanning calorimetry. Qualitative and quantitative information on the material decomposition and transition phases is provided under heating conditions. By monitoring the changes in both mass and internal energy over temperature and time, a couple of thermal data at the maximum decomposition steps allow the creation of a unique and global product identity, depending on both strain and excipient components. We demonstrate that each powder formulation of monostrain and multistrain from different lots and origins have a unique thermophysical profile. Our approach also provides information on the formulation thermostability and additive/excipient composition. An original fingerprint form is proposed by converting the generated thermal data sequence into a star-like pattern for a perspective library construction. Nature Publishing Group UK 2019-07-10 /pmc/articles/PMC6620332/ /pubmed/31292519 http://dx.doi.org/10.1038/s41598-019-46469-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Razafindralambo, Hary
Razafindralambo, Aurélie
Blecker, Christophe
Thermophysical Fingerprinting of Probiotic-Based Products
title Thermophysical Fingerprinting of Probiotic-Based Products
title_full Thermophysical Fingerprinting of Probiotic-Based Products
title_fullStr Thermophysical Fingerprinting of Probiotic-Based Products
title_full_unstemmed Thermophysical Fingerprinting of Probiotic-Based Products
title_short Thermophysical Fingerprinting of Probiotic-Based Products
title_sort thermophysical fingerprinting of probiotic-based products
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620332/
https://www.ncbi.nlm.nih.gov/pubmed/31292519
http://dx.doi.org/10.1038/s41598-019-46469-1
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