Technetium-99m-labeled genistein as a potential radical scavenging agent

The purpose of this study was to determine the optimum conditions for labeling genistein compounds with technetium-99m ((99m)Tc) radionuclides and the percentage of purity obtained in accordance with the requirements of the United State Pharmacopeia. The method used is optimization of several parameters including pH, SnCl(2).2H(2)O as reducing agents, genistein concentration, and incubation time. The results showed that the optimum conditions for labeling (99m)Tc-Genistein were obtained under conditions of pH 8, the amount of SnCl(2).2H(2)O reducing agents was 30 μg, 0.5 mg genistein, and in 10 min. The optimization of this condition resulted in radiochemical purity in the labeling of (99m)Tc-Genistein compounds at 95.43% ± 0.85%. The radiochemical purity of the labeling of (99m)Tc-Genistein compounds has met the requirements of the United State Pharmacopeia as a compound marked for diagnosis of more than 90%.