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Identification of Burkholderia thailandensis with novel genotypes in the soil of central Sierra Leone
BACKGROUND: The soil-dwelling bacillus Burkholderia pseudomallei is the etiological-agent of the neglected and life-threatening emerging infection melioidosis. The distribution of B. pseudomallei in West Africa is unknown. In the present study we aimed to determine whether B. pseudomallei and B. tha...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6623504/ https://www.ncbi.nlm.nih.gov/pubmed/31199807 http://dx.doi.org/10.1371/journal.pntd.0007402 |
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author | Birnie, Emma van ’t Hof, Senne Bijnsdorp, Anne Mansaray, Yembeh Huizenga, Erdi van der Ende, Arie Hugenholtz, Floor Grobusch, Martin P. Wiersinga, W. Joost |
author_facet | Birnie, Emma van ’t Hof, Senne Bijnsdorp, Anne Mansaray, Yembeh Huizenga, Erdi van der Ende, Arie Hugenholtz, Floor Grobusch, Martin P. Wiersinga, W. Joost |
author_sort | Birnie, Emma |
collection | PubMed |
description | BACKGROUND: The soil-dwelling bacillus Burkholderia pseudomallei is the etiological-agent of the neglected and life-threatening emerging infection melioidosis. The distribution of B. pseudomallei in West Africa is unknown. In the present study we aimed to determine whether B. pseudomallei and B. thailandensis are present in the environment of central Sierra Leone. METHODOLOGY/PRINCIPAL FINDINGS: In June-July 2017, we conducted an environmental surveillance study–designed in accordance with existing consensus guidelines—in central Sierra Leone. A total of 1,000 soil samples (100 per site) were collected and cultured. B. pseudomallei was not identified in the soil, but we identified seven novel B. thailandensis sequence types with multi-locus sequence typing (MLST) and 16S rRNA gene sequence analyses. CONCLUSIONS/SIGNIFICANCE: The presence of B. pseudomallei was not demonstrated, however, multiple novel B. thailandensis sequence types were identified. More environmental and sequencing studies are needed to further understand the genetic diversity, evolution and virulence of these emerging organisms. |
format | Online Article Text |
id | pubmed-6623504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-66235042019-07-25 Identification of Burkholderia thailandensis with novel genotypes in the soil of central Sierra Leone Birnie, Emma van ’t Hof, Senne Bijnsdorp, Anne Mansaray, Yembeh Huizenga, Erdi van der Ende, Arie Hugenholtz, Floor Grobusch, Martin P. Wiersinga, W. Joost PLoS Negl Trop Dis Research Article BACKGROUND: The soil-dwelling bacillus Burkholderia pseudomallei is the etiological-agent of the neglected and life-threatening emerging infection melioidosis. The distribution of B. pseudomallei in West Africa is unknown. In the present study we aimed to determine whether B. pseudomallei and B. thailandensis are present in the environment of central Sierra Leone. METHODOLOGY/PRINCIPAL FINDINGS: In June-July 2017, we conducted an environmental surveillance study–designed in accordance with existing consensus guidelines—in central Sierra Leone. A total of 1,000 soil samples (100 per site) were collected and cultured. B. pseudomallei was not identified in the soil, but we identified seven novel B. thailandensis sequence types with multi-locus sequence typing (MLST) and 16S rRNA gene sequence analyses. CONCLUSIONS/SIGNIFICANCE: The presence of B. pseudomallei was not demonstrated, however, multiple novel B. thailandensis sequence types were identified. More environmental and sequencing studies are needed to further understand the genetic diversity, evolution and virulence of these emerging organisms. Public Library of Science 2019-06-14 /pmc/articles/PMC6623504/ /pubmed/31199807 http://dx.doi.org/10.1371/journal.pntd.0007402 Text en © 2019 Birnie et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Birnie, Emma van ’t Hof, Senne Bijnsdorp, Anne Mansaray, Yembeh Huizenga, Erdi van der Ende, Arie Hugenholtz, Floor Grobusch, Martin P. Wiersinga, W. Joost Identification of Burkholderia thailandensis with novel genotypes in the soil of central Sierra Leone |
title | Identification of Burkholderia thailandensis with novel genotypes in the soil of central Sierra Leone |
title_full | Identification of Burkholderia thailandensis with novel genotypes in the soil of central Sierra Leone |
title_fullStr | Identification of Burkholderia thailandensis with novel genotypes in the soil of central Sierra Leone |
title_full_unstemmed | Identification of Burkholderia thailandensis with novel genotypes in the soil of central Sierra Leone |
title_short | Identification of Burkholderia thailandensis with novel genotypes in the soil of central Sierra Leone |
title_sort | identification of burkholderia thailandensis with novel genotypes in the soil of central sierra leone |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6623504/ https://www.ncbi.nlm.nih.gov/pubmed/31199807 http://dx.doi.org/10.1371/journal.pntd.0007402 |
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