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Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats
In this study, transcriptome analysis of PPRV infected PBMC subsets—T helper cells, T cytotoxic cells, monocytes, and B lymphocytes was done to delineate their role in host response. PPRV was found to infect lymphocytes and not monocytes. The established receptor for PPRV—SLAM was found downregulate...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6624447/ https://www.ncbi.nlm.nih.gov/pubmed/31333643 http://dx.doi.org/10.3389/fimmu.2019.01463 |
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author | Wani, Sajad Ahmad Sahu, Amit Ranjan Khan, Raja Ishaq Nabi Pandey, Aruna Saxena, Shikha Hosamani, Neelima Malla, Waseem Akram Chaudhary, Dheeraj Kanchan, Sonam Sah, Vaishali Rajak, Kaushal Kishor Muthuchelvan, D. Mishra, Bina Tiwari, Ashok Kumar Sahoo, Aditya P. Sajjanar, Basavaraj Singh, Yash Pal Gandham, Ravi Kumar Mishra, Bishnu Prasad Singh, Raj Kumar |
author_facet | Wani, Sajad Ahmad Sahu, Amit Ranjan Khan, Raja Ishaq Nabi Pandey, Aruna Saxena, Shikha Hosamani, Neelima Malla, Waseem Akram Chaudhary, Dheeraj Kanchan, Sonam Sah, Vaishali Rajak, Kaushal Kishor Muthuchelvan, D. Mishra, Bina Tiwari, Ashok Kumar Sahoo, Aditya P. Sajjanar, Basavaraj Singh, Yash Pal Gandham, Ravi Kumar Mishra, Bishnu Prasad Singh, Raj Kumar |
author_sort | Wani, Sajad Ahmad |
collection | PubMed |
description | In this study, transcriptome analysis of PPRV infected PBMC subsets—T helper cells, T cytotoxic cells, monocytes, and B lymphocytes was done to delineate their role in host response. PPRV was found to infect lymphocytes and not monocytes. The established receptor for PPRV—SLAM was found downregulated in lymphocytes and non-differentially expressed in monocytes. A profound deviation in the global gene expression profile with a large number of unique upregulated genes (851) and downregulated genes (605) was observed in monocytes in comparison to lymphocytes. ISGs—ISG15, Mx1, Mx2, RSAD2, IFIT3, and IFIT5 that play a role in antiviral response and the genes for viral sensors—MDA5, LGP2, and RIG1, were found to be upregulated in lymphocytes and downregulated in monocytes. The transcription factors—IRF-7 and STAT-1 that regulate expression of most of the ISGs were found activated in lymphocytes and not in monocytes. Interferon signaling pathway and RIG1 like receptor signaling pathway were found activated in lymphocytes and not in monocytes. This contrast in gene expression profiles and signaling pathways indicated the predominant role of lymphocytes in generating the antiviral response against PPRV in goats, thus, giving us new insights into host response to PPRV. |
format | Online Article Text |
id | pubmed-6624447 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66244472019-07-22 Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats Wani, Sajad Ahmad Sahu, Amit Ranjan Khan, Raja Ishaq Nabi Pandey, Aruna Saxena, Shikha Hosamani, Neelima Malla, Waseem Akram Chaudhary, Dheeraj Kanchan, Sonam Sah, Vaishali Rajak, Kaushal Kishor Muthuchelvan, D. Mishra, Bina Tiwari, Ashok Kumar Sahoo, Aditya P. Sajjanar, Basavaraj Singh, Yash Pal Gandham, Ravi Kumar Mishra, Bishnu Prasad Singh, Raj Kumar Front Immunol Immunology In this study, transcriptome analysis of PPRV infected PBMC subsets—T helper cells, T cytotoxic cells, monocytes, and B lymphocytes was done to delineate their role in host response. PPRV was found to infect lymphocytes and not monocytes. The established receptor for PPRV—SLAM was found downregulated in lymphocytes and non-differentially expressed in monocytes. A profound deviation in the global gene expression profile with a large number of unique upregulated genes (851) and downregulated genes (605) was observed in monocytes in comparison to lymphocytes. ISGs—ISG15, Mx1, Mx2, RSAD2, IFIT3, and IFIT5 that play a role in antiviral response and the genes for viral sensors—MDA5, LGP2, and RIG1, were found to be upregulated in lymphocytes and downregulated in monocytes. The transcription factors—IRF-7 and STAT-1 that regulate expression of most of the ISGs were found activated in lymphocytes and not in monocytes. Interferon signaling pathway and RIG1 like receptor signaling pathway were found activated in lymphocytes and not in monocytes. This contrast in gene expression profiles and signaling pathways indicated the predominant role of lymphocytes in generating the antiviral response against PPRV in goats, thus, giving us new insights into host response to PPRV. Frontiers Media S.A. 2019-07-05 /pmc/articles/PMC6624447/ /pubmed/31333643 http://dx.doi.org/10.3389/fimmu.2019.01463 Text en Copyright © 2019 Wani, Sahu, Khan, Pandey, Saxena, Hosamani, Malla, Chaudhary, Kanchan, Sah, Rajak, Muthuchelvan, Mishra, Tiwari, Sahoo, Sajjanar, Singh, Gandham, Mishra and Singh. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Wani, Sajad Ahmad Sahu, Amit Ranjan Khan, Raja Ishaq Nabi Pandey, Aruna Saxena, Shikha Hosamani, Neelima Malla, Waseem Akram Chaudhary, Dheeraj Kanchan, Sonam Sah, Vaishali Rajak, Kaushal Kishor Muthuchelvan, D. Mishra, Bina Tiwari, Ashok Kumar Sahoo, Aditya P. Sajjanar, Basavaraj Singh, Yash Pal Gandham, Ravi Kumar Mishra, Bishnu Prasad Singh, Raj Kumar Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats |
title | Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats |
title_full | Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats |
title_fullStr | Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats |
title_full_unstemmed | Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats |
title_short | Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats |
title_sort | contrasting gene expression profiles of monocytes and lymphocytes from peste-des-petits-ruminants virus infected goats |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6624447/ https://www.ncbi.nlm.nih.gov/pubmed/31333643 http://dx.doi.org/10.3389/fimmu.2019.01463 |
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